LOCUS       V009423                 6623 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009423
VERSION     V009423
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Purton S.
  TITLE     pASapI: an expression vector for the Chlamydomonas chloroplast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Purton S.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6623)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-AUG-2013) Structural "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..25)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        499..603
                     /label="AmpR promoter"
     CDS             604..1461
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1635..2223
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    3200..3798
                     /gene="atpA"
                     /label="promoter and 5'UTR"
                     /note="promoter and 5'UTR"
     gene            3200..3798
                     /gene="atpA"
                     /label="atpA"
     3'UTR           3851..4257
                     /gene="rbcL"
     gene            3851..4257
                     /gene="rbcL"
                     /label="rbcL"
     CDS             complement(4413..4676)
                     /gene="psbH"
                     /label="Photosystem II reaction center protein H"
                     /note="Photosystem II reaction center protein H from
                     Chlamydomonas reinhardtii. Accession#: P22666"
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa cgcgtctcca atatagtaga ctttattaga ggcagtgttt
     3241 atataccata aacgtcaaaa gtcattttta taactggatc tcaaaatacc tataaaccca
     3301 ttgttcttct cttttagctc taagaacaat caatttataa atatatttat tattatgcta
     3361 taatataaat actatataaa tacatttacc tttttataaa tacatttacc ttttttttaa
     3421 tttgcatgat tttaatgctt atgctatctt ttttatttag tccataaaac ctttaaagga
     3481 ccttttctta tgggatattt atattttcct aacaaagcaa tcggcgtcat aaactttagt
     3541 tgcttacgac gcctgtggac gtccccccct tccccttacg ggcaagtaaa cttagggatt
     3601 ttaatgcaat aaataaattt gtcctcttcg ggcaaatgaa ttttagtatt taaatatgac
     3661 aagggtgaac cattactttt gttaacaagt gatcttacca ctcactattt ttgttgaatt
     3721 ttaaacttat ttaaaattct cgagaaagat tttaaaaata aactttttta atcttttatt
     3781 tattttttct tttttatggg aagagctact ccatggatcc tctagagtcg acctgcaggc
     3841 atgcaagctt gtactcaagc tcgtaacgaa ggtcgtgacc ttgctcgtga aggtggcgac
     3901 gtaattcgtt cagcttgtaa atggtctcca gaacttgctg ctgcatgtga agtttggaaa
     3961 gaaattaaat tcgaatttga tactattgac aaactttaat ttttattttt catgatgttt
     4021 atgtgaatag cataaacatc gtttttattt ttatggtgtt taggttaaat acctaaacat
     4081 cattttacat ttttaaaatt aagttctaaa gttatctttt gtttaaattt gcctgtcttt
     4141 ataaattacg atgtgccaga aaaataaaat cttagctttt tattatagaa tttatcttta
     4201 tgtattatat tttataagtt ataataaaag aaatagtaac atactaaagc ggatgtagcg
     4261 cgtttatctt aacggaaggc cagtggcacg tccgcctgcc actgccgaat ataaatatgg
     4321 ttgagttgct tagtttacct tagcgaaaag aagacttagc agctagcctt aacaaacagt
     4381 tttatatttt atgtttgtgt taaataaaat taagaaactt tagctaaagt ttcccaactc
     4441 atagaaacgt catctaaaat taaagaactg ttgtaaattt ctaaaatgat taataagaat
     4501 gctgcaaata aaaggataaa tacagccatt aaaacagttg taccccagcc tggtaatact
     4561 ttacctgctt ctgagttaag tggacgtaat aaagtaccta atggtgtaac taaaccaggt
     4621 tcttggaagt ctgaatttac ttttgatggt ttagctttag aagttcctgt tgccataatt
     4681 gattaaatga attaagcgtt attagcgcta ttttatttac tttctgtaaa aaataaggaa
     4741 aatattcttc agtgcattcc ctctcaggat tataaatact ctgaggataa cgttctctcg
     4801 tcaaggggtt gcttcttgtg agtatagaaa cctactagca caagaaataa attgcataaa
     4861 aatgtattta cctaggaccg cagtaggcag tcccttttcc ccttcagaac tgcctgcttt
     4921 aaaagaatga aaaaactgcc ttgtctggta agtaaaactc tttaattact cactaaagac
     4981 gatcttagaa gttctttgtt cattttttat ttaatataat atttgttata taaaaattaa
     5041 ataattttta attaatgttt aactttgtaa ggacagtttc aaagtgacat gaatggctac
     5101 tgcaaaaacg aagtaagtta ttctttctca gggcaaaatt ttgagtagat taattttgtt
     5161 taaaaatgtg ggacacagtc gtcaagtctt ttgaactatc taagagatat gttgaaaaga
     5221 gaataatttt attattaaat gagctatgga aagtccagct tttttcttta cctttttttt
     5281 atggtttctt ctgttaagtg taactggcta ttcagtttat gttagttttg gtccaccttc
     5341 aaaaaaatta cgtgatcctt ttgaagaaca cgaagattaa acaagttaaa aagtactatt
     5401 tttacaagtg acttcggtgc ctctgagaac cctagttata gtgatataaa ataactagct
     5461 aactacttta tatttttatg aaagtcattt tgtcgagcat ataaacaaaa acaaaattgc
     5521 tatactaggc agtcacagtg caactgtctc cgtctcctta accgagaaag ggtaaacgtc
     5581 ttcggtaaag taacaaactt tagttatgtt aactgcttgc gagttaacca ttttttttcc
     5641 tccgaaggac aacagttggc agttgccaaa ctttagtggt ctaatattta tattaggcag
     5701 ttggcaggca actgcactga cgtcccgaag gggaaggggt ttacttacct cctaacggag
     5761 tatataaata gaataaaatt tatttcctgc gctagcagat ttacatacta ggattttaat
     5821 actccgaagg aggcagtggc ggtaccactg ccactggcgt cctccttccc cttcgggcaa
     5881 atgcatttta gtgccactta agtttacttg cctaggcagt tggcaggacg tcagtggcag
     5941 tggtaccgcg actgcctata tttatatact cctaagttta cttgcctagg cagttggcag
     6001 gcaactgcca ctgacgtcct tccccttccc cttcgggacg tccccttacg ggaatataaa
     6061 tattagtgga tatttatata ctgcgatgtt tacatactcc gaaggaggag agctagcagt
     6121 tgcctgccaa ctgcctaata taaatattgg gcaagtaaac ttagaatgtt tacatactcc
     6181 gaaggaggac gtcccttacg ggaatataaa tattagtggc agtggtaccg ccactgcctc
     6241 cttcggagta ttaaaatcct agtatataat ataccgtaag ggacgtcctc cgacggtggc
     6301 agtggcggta ccactgccac cggcgtccta atatacatat tgaagtattt aaacctgtta
     6361 gcgcacgctc taacgagtca gtaaacttcc cttttggggc ttctaggcag cgcataaatt
     6421 ttctaggacg aacgtccact ggcgtctcgt aaggagcagt gacaggccac taatgtcccc
     6481 ttaatgggta aataaatggc tatcgtctat ccatgaagag accatatatt ccagtagcac
     6541 cgttatgatc ctcaaagggt aacaccattt gtatagtatt atggtgaaat gcatcccttt
     6601 cagggtagat ttatatctta cag
//
