LOCUS       40924_4949        3476 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pASS2SUL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Weeks SD, Drinker M, Loll PJ.
  TITLE     Ligation independent cloning vectors for expression of SUMO fusions
  JOURNAL   Protein Expr. Purif. 53 (1), 40-50 (2007)
  PUBMED    17251035
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Weeks SD, Drinker M, Loll PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2007) Biochemistry and Molecular Biology, Drexel 
            University College of Medicine, 245 N 15th Street, Mailstop 497, 
            Philadelphia, PA 19102, USA
REFERENCE   3  (bases 1 to 3476)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3476)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "1"; pages: 
            "40-50"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2007) Biochemistry and Molecular Biology, Drexel University 
            College of Medicine, 245 N 15th Street, Mailstop 497, Philadelphia, 
            PA 19102, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..91
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             99..122
                     /codon_start=1
                     /product="TetA fragment"
                     /label=TetA fragment
                     /protein_id="ABN58911.1"
                     /translation="MNSSTKI"
     RBS             130..152
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..191
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             192..482
                     /label=SUMO
                     /note="cleavable ubiquitin-like protein tag"
     regulatory      508..579
                     /label=lpp terminator region
                     /note="lpp terminator region"
                     /regulatory_class="terminator"
     rep_origin      587..1042
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1069..1173
                     /label=AmpR promoter
     CDS             1174..2031
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             2041..2664
                     /label=TetR
                     /note="tetracycline repressor TetR"
     rep_origin      2820..3408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccatcgaatg gccagatgat taattcctaa tttttgttga cactctatca ttgatagagt
       61 tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaaaatct
      121 agaaataatt ttgtttaact ttaagaagga gatataccat ggctagctgg agccacccgc
      181 agttcgaaaa atctgactcc gaagtcaatc aagaagctaa gccagaggtc aagccagaag
      241 tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagag atcttcttca
      301 agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa agacagggta
      361 aggaaatgga ctccttaaga ttcttgtacg acggtattag aattcaagcc gatcagaccc
      421 ctgaagattt ggacatggag gataacgata ttattgaggc tcaccgcgaa cagattggtg
      481 gcgtctaaac tctcctcgag aagcttgacc tgtgaagtga aaaatggcgc acattgtgcg
      541 acattttttt tgtctgccgt ttaccgctac tgcgtcacgg atctccacgc gccctgtagc
      601 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      661 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      721 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      781 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      841 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      901 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      961 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     1021 aaaatattaa cgcttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1081 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1141 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1201 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1261 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1321 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1381 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1441 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1501 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1561 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1621 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1681 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1741 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattgat agactggatg
     1801 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1861 gctgataaat ctggagccgg tgagcgtggc tctcgcggta tcattgcagc actggggcca
     1921 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1981 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaggaatta
     2041 atgatgtctc gtttagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
     2101 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
     2161 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
     2221 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
     2281 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
     2341 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
     2401 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc agtggggcat
     2461 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
     2521 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
     2581 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
     2641 caacttaaat gtgaaagtgg gtcttaaaag cagcataacc tttttccgtg atggtaactt
     2701 cactagttta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2941 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgac ccgaca
//
