LOCUS       V009419                 5732 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009419
VERSION     V009419
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Park W-J., Han S-S., Kim H-S., Kim G-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2014) Interdisciplinary Graduate Program in
            Molecular Medicine, Chonnam National University, 77 Yongbong-ro,
            Buk-gu, Gwangju, Jeollanam-Do 500-757, Korea
REFERENCE   2  (bases 1 to 5732)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5732)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (12-AUG-2014) Interdisciplinary Graduate Program in Molecular
            Medicine, Chonnam National University, 77 Yongbong-ro, Buk-gu,
            Gwangju, Jeollanam-Do 500-757, Korea"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..201
                     /label="BEM promoter"
                     /note="BEM promoter"
                     /regulatory_class="promoter"
     CDS             210..959
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     terminator      1030..1116
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(1210..1237)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(1329..1415)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     1764..1780
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1793..2497)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    complement(2504..2591)
                     /label="derived from SodB"
                     /note="derived from SodB"
     protein_bind    complement(2611..2627)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2679..2831
                     /label="luxI promoter"
                     /note="luxI promoter"
                     /regulatory_class="promoter"
     ncRNA           2838..2907
                     /product="RyhB"
                     /label="antisense_RNA"
                     /ncRNA_class="antisense_RNA"
     terminator      complement(2942..2985)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        3144..3246
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3247..3903
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     rep_origin      4640..5228
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5414..5554)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 ttcatatccc ttcgaccaga acacttccat ggccttgcgc aaggcctggg ctcggtcgaa
       61 gttccggggg cgcccacgtt ctgccatgtc gcggatttta ctcgggagaa ctattttgtg
      121 ttgatcgata agaaaatcat tgacgccgcc gtgcatgtcg atggatgata tttgtactga
      181 tcactacaaa ttgaggttga ccggtaccca tgaaaaacat aaatgccgac gacacataca
      241 gaataattaa taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg
      301 atatgactaa aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta
      361 tggttaaatc tgatatttca attctagata attaccctaa aaaatggagg caatattatg
      421 atgacgctaa tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac
      481 caattaattg gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta
      541 aagaagcgaa aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca
      601 atggcttcgg aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat
      661 ttttacatgc gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa
      721 aaataaatat agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt
      781 tagcgtgggc atgcgaagga aaaagctctt gggatatttc aaaaatatta ggctgcagtg
      841 agcgtactgt cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct
      901 gccaaagtat ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt
      961 aagtacggga tccctgcagg cctcagggcc cgatcgatgc ggccgcttaa ttaattaatc
     1021 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1081 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagaa ctagtgggtt
     1141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa aagagtttgt
     1201 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt
     1261 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
     1321 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     1381 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     1441 atggggagac cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
     1501 gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct
     1561 gcgttctgat ttaatctgta tcaggctgaa aatcttatct catccgccaa aacagccaag
     1621 ctgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1681 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1741 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttc tacttgtaca
     1801 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
     1861 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
     1921 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
     1981 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
     2041 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
     2101 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
     2161 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     2221 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     2281 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
     2341 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     2401 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
     2461 tgatgatggc catgttatcc tcctcgccct tgctcacgaa ttcagcatat ggtagtgcag
     2521 gtaattcgaa tgacattgct actctccttt attattaatt tgcatacgta caatagcctt
     2581 attgtgcgta ttctagatgt gctcagtatc ttgttatccg ctcacaatgt caacagctca
     2641 tttcagaata tttgccagaa ccgttatgat gtcggcgcgc tagcccccga aaagtgccac
     2701 ctgacgtcag tcctttgatt ctaataaatt gaatttttgt cacactattg tatcgctggg
     2761 aatacaatta cttaacataa gcacctgtag gatcgtacag gtttacgcaa gaaaatggtt
     2821 tgttatagtc gggatccgcg atcaggaaga ccctcgcgga gaacctgaaa gcacgacatt
     2881 gctcacattg cttccagtat tacttagcca gccgggtgct ggcttttttt ttgggcatca
     2941 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     3001 gaacgctctc ctgagtagga caaatccgcc gccgtcgacg aataaatacc tgtgacggaa
     3061 gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc
     3121 aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat
     3181 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     3241 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     3301 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     3361 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     3421 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     3481 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     3541 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     3601 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     3661 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     3721 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     3781 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     3841 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     3901 gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac gcctgaataa
     3961 gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc
     4021 agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac
     4081 cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc
     4141 cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc tgataaaaac
     4201 ggttagcgct tcgttaataa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4261 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4321 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4381 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4441 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4501 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4561 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5281 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5341 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5401 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5461 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     5521 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     5581 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5641 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat
     5701 caattcgcgc gcgaaggcga agcggcatgc at
//
