LOCUS       V009421                 5758 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009421
VERSION     V009421
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Day WA Jr., Rasmussen SL, Carpenter BM, Peterson SN, Friedlander AM.
  TITLE     Microarray analysis of transposon insertion mutations in Bacillus
            anthracis: global identification of genes required for sporulation
            and germination
  JOURNAL   J. Bacteriol. 189 (8), 3296-3301 (2007)
   PUBMED   17277068
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Day WA Jr., Rasmussen SL, Carpenter BM, Peterson SN, Friedlander AM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2006) Bacteriology Division, USAMRIID, 1425 Porter
            St, Frederick, MD 21702, USA
REFERENCE   3  (bases 1 to 5758)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2007"; volume: "189"; issue: "8"; pages:
            "3296-3301"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-AUG-2006) Bacteriology Division, USAMRIID, 1425 Porter St,
            Frederick, MD 21702, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..24
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     57..73
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             748..1539
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(1879..1895)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(1925..1943)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1979..3484
                     /label="pE194 temperature-sensitive replicon"
                     /note="pE194 temperature-sensitive replicon"
     rep_origin      complement(3667..4255)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4571..5350)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
ORIGIN
        1 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta
       61 gaactagtgg atccggtgat tgattgagca agctttatgc ttgtaaaccg ttttgtgaaa
      121 aaatttttaa aataaaaaag gggacctcta gggtccccaa ttaattagta atataatcta
      181 ttaaaggtca ttcaaaaggt catccaccgg atcgataaac ccagcgaacc atttgaggtg
      241 ataggtaaga ttataccgag gtatgaaaac gagaattgga cctttacaga attactctat
      301 gaagcgccat atttaaaaag ctaccaagac gaagaggatg aagaggatga ggaggcagat
      361 tgccttgaat atattgacaa tactgataag ataatatatc ttttatatag aagatatcgc
      421 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
      481 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
      541 agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata
      601 cgccaacttt caaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
      661 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
      721 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
      781 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
      841 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
      901 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
      961 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
     1021 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     1081 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     1141 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     1201 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
     1261 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     1321 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     1381 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
     1441 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     1501 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc
     1561 taaaaagctt taactacaag ctttttagac atctaatctt ttctgaagta catccgcaac
     1621 tgtccatact ctgatgtttt atatcttttc taaaagttcg ctagataggg gtcccgagcg
     1681 cctacgagga atttgtatcg atgatccggt ggatgacctt ttgaatgacc tttaatagat
     1741 tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa aattttttca
     1801 caaaacggtt tacaagcata aagcttgctc aatcaatcac cggatccccg ggctgcagga
     1861 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccagctttt
     1921 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttccgc
     1981 agtcggctta aaccagtttt cgctggtgcg aaaaaagagt gtcttgtgac acctaaattc
     2041 aaaatctatc ggtcagattt ataccgattt gattttatat attcttgaat aacatacgcc
     2101 gagttatcac ataaaagcgg gaaccaatca tcaaatttaa acttcattgc ataatccatt
     2161 aaactcttaa attctacgat tccttgttca tcaataaact caatcatttc tttaattaat
     2221 ttatatctat ctgttgttgt tttctttaat aattcatcaa catctacacc gccataaact
     2281 atcatatctt ctttttgata tttaaattta ttaggatcgt ccatgtgaag catatatctc
     2341 acaagacctt tcacacttcc tgcaatctgc ggaatagtcg cattcaattc ttctgttaat
     2401 tatttttatc tgttcataag atttattacc ctcatacatc actagaatat gataatgctc
     2461 ttttttcatc ctaccttctg tatcagtatc cctatcatgt aatggagaca ctacaaattg
     2521 aatgtgtaac tcttttaaat actctaacca ctcggctttt gctgattctg gatataaaac
     2581 aaatgtccaa ttacgtcctc ttgaattttt cttgttttca gtttctttta ttacattttc
     2641 gctcatgata taataacggt gctaatacac ttaacaaaat ttagtcatag ataggcagca
     2701 tgccagtgct gtctatcttt ttttgtttaa aatgcaccgt attcctcctt tgcatatttt
     2761 tttattagaa taccggttgc atctgatttg ctaatattat atttttcttt gattctattt
     2821 aatatctcat tttcttctgt tgtaagtctt aaagtaacag caactttttt ctcttctttt
     2881 ctatctacaa ccatcactgt acctcccaac atctgttttt ttcactttaa cataaaaaac
     2941 aaccttttaa cattaaaaac ccaatattta tttatttgtt tggacaatgg acaatggaca
     3001 cctagggggg aggtcgtagt acccccctat gttttctccc ctaaataacc ccaaaaatct
     3061 aagaaaaaaa gacctcaaaa aggtctttaa ttaacatctc aaatttcgca tttattccaa
     3121 tttccttttt gcgtgtgatg cgctgcgtcc attaaaaatc ctagagcttt gcaaccgaaa
     3181 gttaatagct gtcgctacta ctttcgctta cgctctaagt atattttaag gactgtcaca
     3241 cgcaaaaagt tttctcggca taaaagtacc tctacatctc taaatcgtct gtacgctgtt
     3301 tctcacgctt tctatcgata agctagcttt aatgcggtag tttatcacag ttaaattgct
     3361 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
     3421 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
     3481 atgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3841 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4081 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4561 aattagccta attgagagaa gtttctatag aatttttcat atacttaacg agtgctttca
     4621 cctttgaata tagtccttcc cacttatcat cacactctcc ccgatagcct tttctagcta
     4681 tatccagtaa agttacatgc tctttaggta aaagaggtat agcccattct gcagcgacat
     4741 ctttcgaggt aatttcacca gtagtcactg tttgccacat tcgagctagg gttaaaatta
     4801 cattacgctc atcacctttt atcccctcaa ttagttctgg caaagaatcc ttaattgctc
     4861 ttcgaatatc tgtcaaaggt acggagacaa gtatacttga agaatcagga ccaaatagag
     4921 aaatactatt ctttcttgct tgtgctaaaa caatagccaa atcaggatca tagcttggtt
     4981 cctgaatttg tccattctca aattcacccc tgagccactc accgtatata aattctcttt
     5041 ttggaggata ttgccaaggg acaacttcac tcctatttat aaccgtaact tcaagtggtc
     5101 taacagaatc cgtatttcca atctttcctg atatagtcat tagtctttct gttagttttt
     5161 ttcgagttaa ttgaggtaaa ctatgattca cgacgactag aacatctaca tcgctgttaa
     5221 tgcgtaaacc accatttact gctgaaccaa atagatatac tccaactatt gaacttccaa
     5281 ataaatcttt tacgattttt aatgtttgaa tcgcttgatt tggtattttt ccgttaatca
     5341 aattgctcat gatttcacct cgttgattat gttcatataa agtttatatt gatactcaat
     5401 ttacttaccc tagattggac atatacttaa attactgttc aataaagctg accgttagcg
     5461 tttaagtaca tcctttcaca atttgtctac agattaataa ttattcttta ttatacaaaa
     5521 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5581 cgttccactg agcgtcaacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgc
//
