LOCUS       V015887                 5775 bp    DNA     circular SYN 01-MAR-2020
DEFINITION  Exported.
ACCESSION   V015887
VERSION     V015887
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA
            98027, USA
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027,
            USA"
FEATURES             Location/Qualifiers
     source          1..5775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(719..823)
                     /label="AmpR promoter"
     primer_bind     1297..1313
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1421..2530
                     /gene="PSAT1"
                     /label="Phosphoserine aminotransferase"
                     /note="Phosphoserine aminotransferase from Homo sapiens.
                     Accession#: Q9Y617"
     gene            3087..4206
                     /label="natMX6"
                     /note="yeast selectable marker conferring nourseothricin
                     resistance"
     primer_bind     complement(4472..4488)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4496..4512)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4520..4550)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4565..4586)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4874..5462)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(5636..5775,1..718))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
      901 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
      961 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     1021 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     1081 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1141 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     1201 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     1261 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     1321 agctcggtac ccggggcggc cgcctttgaa attacttaat tgaacaacac aggtctaatt
     1381 agttgatcaa tcatcgatta accattagtg ataagaaaca atggacgcgc caagacaggt
     1441 tgtgaatttc ggacctgggc cagctaaatt gcctcactct gttttgttgg aaatccagaa
     1501 agagttatta gattataaag gtgtaggtat atcagtcttg gaaatgtctc accgttcgtc
     1561 tgatttcgcc aaaataatta ataacaccga gaacttagtt agggaactgc tagccgttcc
     1621 tgacaactat aaagtgattt ttttgcaagg tggtggctgc ggtcaatttt ctgccgttcc
     1681 tttaaatttg ataggtttaa aagcgggtag atgtgccgac tatgtcgtca ccggtgcgtg
     1741 gtctgctaaa gccgctgaag aggcaaagaa attcggtact atcaacattg ttcatccaaa
     1801 gttgggctct tacactaaaa ttccggatcc atccacatgg aatttaaatc ccgatgcatc
     1861 atacgtgtac tactgtgcta atgaaactgt tcatggtgtt gaatttgact ttattccaga
     1921 tgttaagggt gctgttttgg tttgcgacat gtccagcaac ttcttgtcta agccagttga
     1981 cgtttctaaa ttcggtgtca tctttgcagg cgcccaaaaa aacgttggtt ctgcaggcgt
     2041 aaccgtggtt attgtcagag atgacttact tggttttgcg ttaagagaat gcccttctgt
     2101 tttagagtat aaggtgcagg ccggtaactc atcactgtat aatacaccac catgcttctc
     2161 tatatatgtt atgggtctcg tgttagaatg gattaagaat aatggtggtg cagctgcgat
     2221 ggagaaattg tcttctatta agtctcagac gatatatgag ataatagata actcacaggg
     2281 tttttacgtt tgcccagtgg aaccacagaa tagatcaaaa atgaatatcc cattccgtat
     2341 aggtaacgcg aagggcgatg acgcccttga aaagaggttt ctagataagg cacttgagtt
     2401 aaatatgctg tctttgaaag gacatcgttc agttggtggg atcagagcct ccttgtataa
     2461 cgcggtgacg atcgaagacg tacaaaagtt ggccgctttt atgaagaaat ttttggagat
     2521 gcaccagtta taatatcagc ggaatatgcc tgtaatttgg ttcaaagaaa aactttttca
     2581 agtcatatgt aagttttaaa taaatcagtt tacgaacatg cacaagaata acttctcttc
     2641 tttttcttga aataaaggcc gaattatacg atatgatatg agacgttcta attacaatac
     2701 gataccgtga gataaaaata gcgatatcct cgtactcaaa gaagtctaat tgttttagcc
     2761 cgttgatcaa aaaaataaaa tgtattttta tttttttgtc atccagctca agatcgtaat
     2821 attggcaatt aacaaccgag cgaggcacag ggcaaagaga atgtgggtga gactgaacta
     2881 tctctcccaa tatattatac ttttttttgt cgcgtctacc aattaaatga aaacataatt
     2941 gggaatactt tcctcttctt atattttatc ttaactcgtt tccgatcttc tgatatcgta
     3001 cgctgcaggt cgacggatcc ccgggttaat taaggcgcgc cagatctgtt tagcttgcct
     3061 tgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
     3121 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
     3181 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
     3241 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacaaac gcgttgaatt
     3301 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
     3361 tctttcatat acttcctttt taaatcttgc taggatacag ttctcacatc acatccgaac
     3421 ataaacaacc atgggtacca ctcttgacga cacggcttac cggtaccgca ccagtgtccc
     3481 gggggacgcc gaggccatcg aggcactgga tgggtccttc accaccgaca ccgtcttccg
     3541 cgtcaccgcc accggggacg gcttcaccct gcgggaggtg ccggtggacc cgcccctgac
     3601 caaggtgttc cccgacgacg aatcggacga cgaatcggac gacggggagg acggcgaccc
     3661 ggactctcgg acgttcgtcg cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt
     3721 ctcgtactcc ggctggaacc gccggctgac cgtcgaggac atcgaggtcg ccccggagca
     3781 ccgggggcac ggggtcgggc gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg
     3841 gggtgccggg cacctctggc tggaggtcac caacgtcaac gcaccggcga tccacgcgta
     3901 ccggcggatg gggttcaccc tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc
     3961 ggacggcgag caggcgctct acatgagcat gccctgcccc taatcagtac tgacaataaa
     4021 aagattcttg ttttcaagaa cttgtcattt gtatagtttt tttatattgt agttgttcta
     4081 ttttaatcaa atgttagcgt gatttatatt ttttttcgcc tcgacatcat ctgcccagat
     4141 gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct
     4201 atactgctgt cgattcgata ctaacgccgc catccagtgt cgaaaacgag ctcgaattca
     4261 tcgataagaa gtatctcttt tcaagaggat tacttttcat tgaaaacttt tggacgggca
     4321 aaaaaaaaaa agcgccagca ggaagaaaaa aggaatctag taaaaaataa aaattaggtt
     4381 tataaagtag taagtgaagt gcaagaggga gcgttattgg acgatcatat tgtggcggcc
     4441 gctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4501 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4561 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4621 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4681 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4741 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4801 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5281 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5461 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5521 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5581 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5641 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5761 caatgatacc gcgag
//
