LOCUS       62056_3185        5631 bp DNA     circular SYN 10-NOV-2020
DEFINITION  Cloning vector pAS36_yDUT_HO, complete sequence.
ACCESSION   MT684465
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2020) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 
            98027, USA
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2020) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027, 
            USA"
FEATURES             Location/Qualifiers
     source          1..5631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(527..1384)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1385..1489)
                     /label=AmpR promoter
     primer_bind     1963..1979
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3405..3748
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      4332..4519
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4994..5010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5018..5034)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5042..5072)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5087..5108)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(join(5396..5631,1..353))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
       61 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1501 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1561 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1621 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1681 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     1741 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1801 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1861 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1921 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     1981 aattcgagct cggtacccgg ggcggccgcc gtcacggcta actcttacgt tatgtgcgca
     2041 gatggctccg ctgcccgcgt cataaatgtc acacagggct atcagaaaat ctataatata
     2101 cagcaaaaaa ccaaacacag agcttttgaa ggtgaacctg gtaggttaga tcccaggcgt
     2161 agaacagttt atcagcgtct tgcattacaa tgtactgcag gtcataaatt gtcagtcagg
     2221 gtccctacca aaccactgtt ggaaaaaagt ggtagaaatg ccaccaaata taaagtgaga
     2281 tggagaaatc tgcagcaatg tcagacgctt gatggtagag cctatgtaca tacacacgca
     2341 ccattatctc tcgttttaca taagtaaata cagcaataat aatacctgta aatatctcaa
     2401 catactcaat caaatgagct gataagcata ttcaattttt cttcatagca atattttttt
     2461 ctgtccaagt tttatttttt ttccataatt tctgatttac taccattgaa aattataaaa
     2521 ggaaaaatat tacgcgctac catttaataa gtagaaacaa ctaagttctc gaatgagatg
     2581 tttgcactga tacagcgagc caaaaaataa ccaaatgccc tgcagtgaag agacccctgc
     2641 aatttctcct tccaagcgtg ccagaccggc tgaagtgggg ggcatgcaac ttagatttgc
     2701 cagactttca gaacacgcaa cggcaccgac gagagggagt gcgagggccg ctgggtacga
     2761 cctttacagt gcttatgact acactatccc tccgatggaa aaggcagtag taaaaacgga
     2821 tattcaaatt gctctgcctt cagggtgtta tggtcgtgtc gccccgaggt ctggactggc
     2881 ggcgaagcac tttattgacg taggtgcagg ggttattgat gaagactata ggggcaatgt
     2941 tggagtagtt ctatttaact ttggcaagga gaagtttgaa gtgaagaaag gcgaccgaat
     3001 tgcccaacta atttgtgaac gtattttcta tccagaaatt gaggaggttc aggcgctgga
     3061 tgacacggaa aggggaagtg ggggtttcgg aagcacaggc aagaattaac ttagtgtata
     3121 tactttggca cacttgtata atgtataata aaatcaggat aaatccagtg tgacccggac
     3181 tgaattactg aaactttgaa gtgttaagga aattgtactg ccatttaacg catttaccta
     3241 tcacttagta gcatgcataa gccatgggct aatcataaca gattgtgatg ataggcatcc
     3301 tgtactcctt tgctcgtacg ctgcaggtcg acggatcccc gggttaatta aggcgcgcca
     3361 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
     3421 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
     3481 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
     3541 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
     3601 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
     3661 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
     3721 ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg
     3781 gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac
     3841 caccgacacc gtcttccgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc
     3901 ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga
     3961 cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct
     4021 ggcgggcttc gtggtcatct cgtactcggc gtggaaccgc cggctgaccg tcgaggacat
     4081 cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac
     4141 ggagttcgcc ggcgagcggg gcgccgggca cctctggctg gaggtcacca acgtcaacgc
     4201 accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct
     4261 gtacgacggc accgcctcgg acggcgagcg gcaggcgctc tacatgagca tgccctgccc
     4321 ctaatctcga ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
     4381 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttgttct
     4441 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta
     4501 ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcaccc
     4561 aattgtagat atgctaactc cagcaatgag ttgatgaatc tcggtgtgta ttttatgtcc
     4621 tcagaggaca acacctgttg taatcgagct cgaattcatc gatgcactgt gttgcttgtc
     4681 ccagaaaggg aatcaagtat ttttataaag attggagtgg taaaaatcga gtatgtgcta
     4741 gatgctatgg aagatacaaa ttcagcggtc atcactgtat aaattgcaag tatgtaccag
     4801 aagcacgtga agtgaaaaag gcaaaagaca aaggcgaaaa attgggcatt acgcccgaag
     4861 gtttgccagt taaaggacca gagtgtataa aatgtggcgg aatcttacag tttgatgctg
     4921 tccgcgggcc tcataagagt tgtggtaaca acgcaggcgg ccgctgcagg catgcaagct
     4981 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5041 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5101 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5161 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5221 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5281 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5341 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5401 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5461 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5521 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5581 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt c
//
