LOCUS       V015499                 5580 bp    DNA     circular SYN 10-NOV-2020
DEFINITION  Exported.
ACCESSION   V015499
VERSION     V015499
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2020) Dudley Lab, PNRI, 720 Broadway, Seattle, WA
            98027, USA
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2020) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027,
            USA"
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(527..1384)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1385..1489)
                     /label="AmpR promoter"
     primer_bind     1963..1979
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2615..3055
                     /gene="DUT1"
                     /label="Deoxyuridine 5'-triphosphate nucleotidohydrolase"
                     /note="Deoxyuridine 5'-triphosphate nucleotidohydrolase
                     from Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P33317"
     promoter        3354..3697
                     /label="TEF promoter"
                     /note="Ashbya gossypii TEF promoter"
     terminator      4281..4468
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4943..4959)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4967..4983)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4991..5021)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5036..5057)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(join(5345..5580,1..353))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
       61 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1501 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     1561 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     1621 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     1681 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     1741 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1801 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1861 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1921 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     1981 aattcgagct cggtacccgg ggcggccgcc gtcacggcta actcttacgt tatgtgcgca
     2041 gatggctccg ctgcccgcgt cataaatgtc acacagggct atcagaaaat ctataatata
     2101 cagcaaaaaa ccaaacacag agcttttgaa ggtgaacctg gtaggttaga tcccaggcgt
     2161 agaacagttt atcagcgtct tgcattacaa tgtactgcag gtcataaatt gtcagtcagg
     2221 gtccctacca aaccactgtt ggaaaaaagt ggtagaaatg ccaccaaata taaagtgaga
     2281 tggagaaatc tgcagcaatg tcagacgctt gatggtagag cctatgtaca tacacacgca
     2341 ccattatctc tcgttttaca taagtaaata cagcaataat aatacctgta aatatctcaa
     2401 catactcaat caaatgagct gataagcata ttcaattttt cttcatagca atattttttt
     2461 ctgtccaagt tttatttttt ttccataatt tctgatttac taccattgaa aattataaaa
     2521 ggaaaaatat tacgcgctac catttaataa gtagaaacaa ctaagttctc gaatgagatg
     2581 tttgcactga tacagcgagc caaaaaataa ccaaatgact gctactagcg acaaagtact
     2641 aaagattcaa ttgcgctcag caagcgctac tgtacctacc aaaggttctg ccactgccgc
     2701 gggatacgac atttatgcat ctcaggatat taccattccg gctatgggtc aaggtatggt
     2761 ttccaccgac atatcgttca ccgtacctgt tggtacctac ggtcgtattg cgccaaggtc
     2821 aggcctggca gtgaaaaacg gtatccaaac cggtgctggt gttgtcgaca gagattacac
     2881 cggtgaagtt aaagtagttt tattcaatca ttcacagagg gatttcgcga tcaaaaaagg
     2941 tgatcgcgta gcccaattga ttctggaaaa aattgtcgat gatgcccaga tcgttgttgt
     3001 agactctctg gaagaaagtg caagaggggc cggtggcttt ggtagcactg gtaactaact
     3061 tagtgtatat actttggcac acttgtataa tgtataataa aatcaggata aatccagtgt
     3121 gacccggact gaattactga aactttgaag tgttaaggaa attgtactgc catttaacgc
     3181 atttacctat cacttagtag catgcataag ccatgggcta atcataacag attgtgatga
     3241 taggcatcct gtactccttt gctcgtacgc tgcaggtcga cggatccccg ggttaattaa
     3301 ggcgcgccag atctgtttag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg
     3361 aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact
     3421 gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc atacattttg
     3481 atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc gagcagggaa
     3541 acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa
     3601 aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag
     3661 gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaccactc ttgacgacac
     3721 ggcttaccgg taccgcacca gtgtcccggg ggacgccgag gccatcgagg cactggatgg
     3781 gtccttcacc accgacaccg tcttccgcgt caccgccacc ggggacggct tcaccctgcg
     3841 ggaggtgccg gtggacccgc ccctgaccaa ggtgttcccc gacgacgaat cggacgacga
     3901 atcggacgac ggggaggacg gcgacccgga ctcccggacg ttcgtcgcgt acggggacga
     3961 cggcgacctg gcgggcttcg tggtcatctc gtactcggcg tggaaccgcc ggctgaccgt
     4021 cgaggacatc gaggtcgccc cggagcaccg ggggcacggg gtcgggcgcg cgttgatggg
     4081 gctcgcgacg gagttcgccg gcgagcgggg cgccgggcac ctctggctgg aggtcaccaa
     4141 cgtcaacgca ccggcgatcc acgcgtaccg gcggatgggg ttcaccctct gcggcctgga
     4201 caccgccctg tacgacggca ccgcctcgga cggcgagcgg caggcgctct acatgagcat
     4261 gccctgcccc taatctcgag gcgaatttct tatgatttat gatttttatt attaaataag
     4321 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     4381 tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     4441 tcgctcttat tgaccacacc tctaccggca tgccgagcaa atgcctgcaa atcgctcccc
     4501 atttcaccca attgtagata tgctaactcc agcaatgagt tgatgaatct cggtgtgtat
     4561 tttatgtcct cagaggacaa cacctgttgt aatcgagctc gaattcatcg atgcactgtg
     4621 ttgcttgtcc cagaaaggga atcaagtatt tttataaaga ttggagtggt aaaaatcgag
     4681 tatgtgctag atgctatgga agatacaaat tcagcggtca tcactgtata aattgcaagt
     4741 atgtaccaga agcacgtgaa gtgaaaaagg caaaagacaa aggcgaaaaa ttgggcatta
     4801 cgcccgaagg tttgccagtt aaaggaccag agtgtataaa atgtggcgga atcttacagt
     4861 ttgatgctgt ccgcgggcct cataagagtt gtggtaacaa cgcaggcggc cgctgcaggc
     4921 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4981 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5041 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5101 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
//
