LOCUS       V009431                10805 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009431
VERSION     V009431
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(965..1012)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1603..1883
                     /label="TRP1 promoter"
     CDS             1884..2555
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(2661..3116)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3261..3277
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(3314..3561)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3858..4304)
                     /gene="RPL28"
                     /label="Large ribosomal subunit protein uL15"
                     /note="Large ribosomal subunit protein uL15 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P02406"
     promoter        4769..5473
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5501..5941
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             5957..5983
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5999..8368
                     /gene="TNFAIP3"
                     /label="Tumor necrosis factor alpha-induced protein 3"
                     /note="Tumor necrosis factor alpha-induced protein 3 from
                     Homo sapiens. Accession#: P21580"
     terminator      8443..8630
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8654..8670)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8678..8694
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8702..8732)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8747..8768)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9056..9644)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9818..10675)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10676..10780)
                     /label="AmpR promoter"
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcttgg gtggtcatat
     6001 ggctgaacaa gtccttcctc aggctttgta tttgagcaat atgcggaaag ctgtgaagat
     6061 acgggagaga actccagaag acatttttaa acctactaat gggatcattc atcattttaa
     6121 aaccatgcac cgatacacac tggaaatgtt cagaacttgc cagttttgtc ctcagtttcg
     6181 ggagatcatc cacaaagccc tcatcgacag aaacatccag gccaccctgg aaagccagaa
     6241 gaaactcaac tggtgtcgag aagtccggaa gcttgtggcg ctgaaaacga acggtgacgg
     6301 caattgcctc atgcatgcca cttctcagta catgtggggc gttcaggaca cagacttggt
     6361 actgaggaag gcgctgttca gcacgctcaa ggaaacagac acacgcaact ttaaattccg
     6421 ctggcaactg gagtctctca aatctcagga atttgttgaa acggggcttt gctatgatac
     6481 tcggaactgg aatgatgaat gggacaatct tatcaaaatg gcttccacag acacacccat
     6541 ggcccgaagt ggacttcagt acaactcact ggaagaaata cacatatttg tcctttgcaa
     6601 catcctcaga aggccaatca ttgtcatttc agacaaaatg ctaagaagtt tggaatcagg
     6661 ttccaatttc gcccctttga aagtgggtgg aatttacttg cctctccact ggcctgccca
     6721 ggaatgctac agatacccca ttgttctcgg ctatgacagc catcattttg tacccttggt
     6781 gaccctgaag gacagtgggc ctgaaatccg agctgttcca cttgttaaca gagaccgggg
     6841 aagatttgaa gacttaaaag ttcacttttt gacagatcct gaaaatgaga tgaaggagaa
     6901 gctcttaaaa gagtacttaa tggtgataga aatccccgtc caaggctggg accatggcac
     6961 aactcatctc atcaatgccg caaagttgga tgaagctaac ttaccaaaag aaatcaatct
     7021 ggtagatgat tactttgaac ttgttcagca tgagtacaag aaatggcagg aaaacagcga
     7081 gcaggggagg agagaggggc acgcccagaa tcccatggaa ccttccgtgc cccagctttc
     7141 tctcatggat gtaaaatgtg aaacgcccaa ctgccccttc ttcatgtctg tgaacaccca
     7201 gcctttatgc catgagtgct cagagaggcg gcaaaagaat caaaacaaac tcccaaagct
     7261 gaactccaag ccgggccctg aggggctccc tggcatggcg ctcggggcct ctcggggaga
     7321 agcctatgag cccttggcgt ggaaccctga ggagtccact ggggggcctc attcggcccc
     7381 accgacagca cccagccctt ttctgttcag tgagaccact gccatgaagt gcaggagccc
     7441 cggctgcccc ttcacactga atgtgcagca caacggattt tgtgaacgtt gccacaacgc
     7501 ccggcaactt cacgccagcc acgccccaga ccacacaagg cacttggatc ccgggaagtg
     7561 ccaagcctgc ctccaggatg ttaccaggac atttaatggg atctgcagta cttgcttcaa
     7621 aaggactaca gcagaggcct cctccagcct cagcaccagc ctccctcctt cctgtcacca
     7681 gcgttccaag tcagatccct cgcggctcgt ccggagcccc tccccgcatt cttgccacag
     7741 agctggaaac gacgcccctg ctggctgcct gtctcaagct gcacggactc ctggggacag
     7801 gacggggacg agcaagtgca gaaaagccgg ctgcgtgtat tttgggactc cagaaaacaa
     7861 gggcttttgc acactgtgtt tcatcgagta cagagaaaac aaacattttg ctgctgcctc
     7921 agggaaagtc agtcccacag cgtccaggtt ccagaacacc attccgtgcc tggggaggga
     7981 atgcggcacc cttggaagca ccatgtttga aggatactgc cagaagtgtt tcattgaagc
     8041 tcagaatcag agatttcatg aggccaaaag gacagaagag caactgagat cgagccagcg
     8101 cagagatgtg cctcgaacca cacaaagcac ctcaaggccc aagtgcgccc gggcctcctg
     8161 caagaacatc ctggcctgcc gcagcgagga gctctgcatg gagtgtcagc atcccaacca
     8221 gaggatgggc cctggggccc accggggtga gcctgccccc gaagaccccc ccaagcagcg
     8281 ttgccgggcc cccgcctgtg atcattttgg caatgccaag tgcaacggct actgcaacga
     8341 atgctttcag ttcaagcaga tgtatggcta accggaaaca ggtgggtcac ctcctgcaag
     8401 aagtggggcc tcgaggtcga cctgcagcca agctaattcc gggcgaattt cttatgattt
     8461 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     8521 tcttaggttt taaaacgaaa attcttgttc ttgagtaact ctttcctgta ggtcaggttg
     8581 ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcaagct
     8641 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8701 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgaggtaac
     8761 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8821 tggattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9481 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10801 cacct
//
