LOCUS       V009434                10116 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009434
VERSION     V009434
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10116)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10116)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(965..1012)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(1060..1164)
                     /label="FLP terminator"
                     /note="FLP terminator"
                     /regulatory_class="terminator"
     promoter        1603..1883
                     /label="TRP1 promoter"
     CDS             1884..2555
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(2661..3116)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3261..3277
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(3314..3561)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3858..4304)
                     /gene="RPL28"
                     /label="Large ribosomal subunit protein uL15"
                     /note="Large ribosomal subunit protein uL15 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P02406"
     promoter        4769..5473
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5501..5941
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             5993..6388
                     /codon_start=1
                     /note="unnamed protein product; hMLT fragment"
                     /protein_id="SJL86956.1"
                     /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSELL
                     DQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCTVT
                     ELSDFLQAMEHTEVLQLLSPPGIKITVN"
     misc_feature    5996..5998
                     /label="Kozak"
                     /note="Kozak"
     regulatory      6416..6517
                     /label="ADH1 terminator"
                     /note="ADH1 terminator"
                     /regulatory_class="terminator"
     CDS             complement(6584..7792)
                     /codon_start=1
                     /note="unnamed protein product; IS10R"
                     /protein_id="SJL86957.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ
                     KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQL
                     VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ
                     KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYAL
                     GKL"
     primer_bind     complement(7965..7981)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(7970..8007)
                     /label="5' UTR of lacZ, incl. RBS"
                     /note="5' UTR of lacZ, incl. RBS"
     protein_bind    7989..8005
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8013..8043)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8058..8079)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8367..8955)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9129..9986)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9987..10091)
                     /label="AmpR promoter"
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     5941 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcatggcgct
     6001 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc
     6061 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga
     6121 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag
     6181 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga
     6241 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt
     6301 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag
     6361 ccccccagga ataaagatta ctgtaaacgt cgacctgcag ccaagctaat tccgggcgaa
     6421 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     6481 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     6541 gtaggtcagg ttgctttctc aggtatagca ctgagagatc ccctcataat ttccccaaag
     6601 cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc agccacgagt aagtcttccc
     6661 ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc taagcgaact gttgagagta
     6721 cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc ccaaccttgt ttctgagcat
     6781 gaacgcccgc aagccaacat gttagttgaa gcatcagggc gattagcagc atgatatcaa
     6841 aacgctctga gctgctcgtt cggctatggc gtaggcctag tccgtaggca ggacttttca
     6901 agtctcggaa ggtttcttca atctgcattc gcttcgaata gatattaaca agttgtttgg
     6961 gtgttcgaat ttcaacaggt aagttagttg ctagaaccca tggctccttt gccgacgctg
     7021 agtagatttt aggtgacggg tggtgacaat gagtccgtgt cgagcgctga ttttttcggc
     7081 ctttagagcg agatttatac aatagaattt ggcatgagat tggattgctt ttagtcagcc
     7141 tcttatagcc taaagtcttt gagtgactag atgacatatc atgtaagttg ctgataggtt
     7201 tccagttttc cgctcctagg tctgcatatt gtacttttcc tcttactcga cttaaccagt
     7261 accaacccag cttctcaacg gatttatacc atggcacttt aaagccagca tcactgacaa
     7321 tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc ggctagaaat tggtcatgag
     7381 ctttctttga acattgctct gaaagcggga acgctttctc ataaagagta acagaacgac
     7441 cgtgtagtgc gactgaagct cgcaatacca taagtcgttt ttgctcacga atatcagacc
     7501 agtcaacaag tacaatgggc atcgtattgc ccgaacagat aaagctagca tgccaacggt
     7561 atacagcgag tcgctctttg tggaggtgac gattacctaa caatcggtcg attcgtttga
     7621 tgttatgttt tgttctcgct ttggttggca ggttacggcc aagttcggta agagtgagag
     7681 ttttacagtc aagtaatgcg tggcaagcca acgttaagct gttgagtcgt tttaagtgta
     7741 attcggggca gaattggtaa agagagtcgt gtaaaatatc gagttcgcac atcttgttgt
     7801 ctgattattg atttttcgcg aaaccatttg atcatatgac aagatgtgta tccaccttaa
     7861 cttaatgatt tttaccaaaa tcattagggg attcatcagg gtatagcatg aggtcgctct
     7921 tattgaccac acctctaccg gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     7981 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8041 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
     8101 gctttccagt cgggaaacct gtcgtgccag ctggattaat gaatcggcca acgcgcgggg
     8161 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8701 ttatcgccac tggcagcagc cactagtaac aggattagca gagcgaggta tgtaggcggt
     8761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9001 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9061 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9121 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9181 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9241 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9301 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9361 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9421 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9481 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9541 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9601 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9661 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9721 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9781 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9841 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9901 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9961 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10021 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10081 ggggttccgc gcacatttcc ccgaaaagtg ccacct
//
