LOCUS       V009436                10338 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009436
VERSION     V009436
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10338)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(965..1012)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(1060..1164)
                     /label="FLP terminator"
                     /note="FLP terminator"
                     /regulatory_class="terminator"
     promoter        1603..1883
                     /label="TRP1 promoter"
     CDS             1884..2555
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(2661..3116)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3261..3277
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(3314..3561)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3858..4304)
                     /gene="RPL28"
                     /label="Large ribosomal subunit protein uL15"
                     /note="Large ribosomal subunit protein uL15 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P02406"
     promoter        4769..5473
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5501..5941
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             5993..6610
                     /codon_start=1
                     /note="unnamed protein product; hMLT fragment"
                     /protein_id="SJL86962.1"
                     /translation="VEIIIGRTDEAVECTEDELNNLGHPDNKEQTTDQPLAKDKVALLI
                     GNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVY
                     GLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRK
                     RNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANG"
     misc_feature    6569..6571
                     /label="stability creator"
                     /note="stability creator"
     regulatory      6638..6739
                     /label="ADH1 terminator"
                     /note="ADH1 terminator"
                     /regulatory_class="terminator"
     CDS             complement(6806..8014)
                     /codon_start=1
                     /note="unnamed protein product; IS10R"
                     /protein_id="SJL86963.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQ
                     KRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQL
                     VNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQ
                     KQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYAL
                     GKL"
     primer_bind     complement(8187..8203)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(8192..8229)
                     /label="5' UTR of lacZ, incl. RBS"
                     /note="5' UTR of lacZ, incl. RBS"
     protein_bind    8211..8227
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8235..8265)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8280..8301)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8589..9177)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9351..10208)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10209..10313)
                     /label="AmpR promoter"
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     5941 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcgtagaaat
     6001 catcatagga agaacagatg aggcagtgga gtgcactgaa gatgaattaa ataatcttgg
     6061 tcatcctgat aataaagagc aaacaactga ccagcctttg gcgaaggaca aggttgccct
     6121 tttgatagga aatatgaatt accgggagca ccccaagctc aaagctcctt tggtggatgt
     6181 gtacgaattg actaacttac tgagacagct ggacttcaaa gtggtttcac tgttggatct
     6241 tactgaatat gagatgcgta atgctgtgga tgagttttta ctccttttag acaagggagt
     6301 atatgggtta ttatattatg caggacatgg ttatgaaaat tttgggaaca gcttcatggt
     6361 ccccgttgat gctccaaatc catataggtc tgaaaattgt ctgtgtgtac aaaatatact
     6421 gaaattgatg caagaaaaag aaactggact taatgtgttc ttattggata tgtgtaggaa
     6481 aagaaatgac tacgatgata ccattccaat cttggatgca ctaaaagtca ccgccaatat
     6541 tgtgtttgga tatgccacgt gtcaaggagc tgaagctttt gaaatccagc attctggatt
     6601 ggcaaatgga gtcgacctgc agccaagcta attccgggcg aatttcttat gatttatgat
     6661 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     6721 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     6781 tcaggtatag cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     6841 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     6901 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     6961 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     7021 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     7081 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     7141 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     7201 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     7261 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     7321 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     7381 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     7441 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     7501 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     7561 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     7621 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     7681 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     7741 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     7801 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     7861 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     7921 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg cagaattggt
     7981 aaagagagtc gtgtaaaata tcgagttcgc acatcttgtt gtctgattat tgatttttcg
     8041 cgaaaccatt tgatcatatg acaagatgtg tatccacctt aacttaatga tttttaccaa
     8101 aatcattagg ggattcatca gggtatagca tgaggtcgct cttattgacc acacctctac
     8161 cggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8221 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8281 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8341 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8941 gccactagta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9001 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10321 ccccgaaaag tgccacct
//
