LOCUS       V009437                10353 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009437
VERSION     V009437
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10353)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(965..1012)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(1060..1164)
                     /label="FLP terminator"
                     /note="FLP terminator"
                     /regulatory_class="terminator"
     promoter        1603..1883
                     /label="TRP1 promoter"
     CDS             1884..2555
                     /label="TRP1"
                     /note="phosphoribosylanthranilate isomerase, required for
                     tryptophan biosynthesis"
     rep_origin      complement(2661..3116)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3261..3277
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      complement(3314..3561)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3858..4304)
                     /gene="RPL28"
                     /label="Large ribosomal subunit protein uL15"
                     /note="Large ribosomal subunit protein uL15 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P02406"
     promoter        4769..5473
                     /label="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             5501..5941
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             5957..5983
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             5987..7954
                     /gene="Ripk1"
                     /label="Receptor-interacting serine/threonine-protein
                     kinase 1"
                     /note="Receptor-interacting serine/threonine-protein kinase
                     1 from Mus musculus. Accession#: Q60855"
     terminator      7991..8178
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8202..8218)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8226..8242
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8250..8280)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8295..8316)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8604..9192)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9366..10223)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10224..10328)
                     /label="AmpR promoter"
ORIGIN
        1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
       61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
      121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
      181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
      241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
      301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
      361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
      421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
      481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
      541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
      601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
      661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
      721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
      781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
      841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
      901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
      961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
     1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
     1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
     1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
     1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
     1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
     1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
     1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
     1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
     2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
     2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
     2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
     2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
     2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
     2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
     2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
     2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
     2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
     2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
     2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
     2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
     3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
     3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
     3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
     3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
     3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
     3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
     3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
     3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
     3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
     3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
     3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
     3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc
     3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
     4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
     4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
     4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
     4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
     4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
     4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
     4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
     4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
     4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
     4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
     4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
     4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
     4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
     4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
     4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
     5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
     5221 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
     5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
     5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
     5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
     5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
     5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     5941 gccggaattc atggcttacc catacgatgt tccagattac gctagcatgc aaccagacat
     6001 gtccttggac aatattaaga tggcatccag tgacctgctg gagaagacag acctagacag
     6061 cggaggcttc gggaaggtgt ccttgtgtta ccacagaagc catggatttg tcatcctgaa
     6121 aaaagtatac acagggccca accgcgctga gtacaatgag gttctcttgg aagaggggaa
     6181 gatgatgcac agactgagac acagtcgagt ggtgaagcta ctgggcatca tcatagaaga
     6241 agggaactat tcgctggtga tggagtacat ggagaagggc aacctgatgc acgtgctaaa
     6301 gacccagata gatgtcccac tttcattgaa aggaaggata atcgtggagg ccatagaagg
     6361 catgtgctac ttacatgaca aaggtgtgat acacaaggac ctgaagcctg agaatatcct
     6421 cgttgatcgt gactttcaca ttaagatagc cgatcttggt gtggcttcct ttaagacatg
     6481 gagcaaactg actaaggaga aagacaacaa gcagaaagaa gtgagcagca ccactaagaa
     6541 gaacaatggt ggtacccttt actacatggc acccgaacac ctgaatgaca tcaatgcaaa
     6601 gcccacggag aagtcggacg tgtacagctt tggcattgtc ctttgggcaa tatttgcaaa
     6661 aaaggagccc tatgagaatg tcatctgtac tgagcagttc gtgatctgca taaaatctgg
     6721 gaacaggcca aatgtagagg aaatccttga gtactgtcca agggagatca tcagcctcat
     6781 ggagcggtgc tggcaggcga tcccagaaga caggccaaca tttcttggca ttgaagaaga
     6841 atttaggcct ttttacttaa gtcattttga agaatatgta gaagaggatg tggcaagttt
     6901 aaagaaagag tatccagatc aaagcccagt gctgcagaga atgttttcac tgcagcatga
     6961 ctgtgtaccc ttacctccga gcaggtcaaa ttcagaacaa cctggatcgc tgcacagttc
     7021 ccaggggctc cagatgggtc ctgtggagga gtcctggttt tcttcctccc cagagtaccc
     7081 acaggacgag aatgatcgca gtgtgcaggc taagctgcaa gaggaagcca gctatcatgc
     7141 ttttggaata tttgcagaga aacagacaaa accgcagcca aggcagaatg aggcttacaa
     7201 cagagaggag gaaaggaaac gaagggtctc tcatgacccc tttgcacagc agagagctcg
     7261 tgagaatatt aagagtgcag gagcaagagg tcattctgat cccagcacaa cgagtcgtgg
     7321 aattgcagtg caacagctgt catggccagc cacccaaaca gtttggaaca atggattgta
     7381 taatcagcat ggatttggaa ctacaggtac aggagtttgg tatccgccaa atctaagcca
     7441 aatgtatagt acttataaaa ctccagtgcc agagaccaac ataccgggaa gcacacccac
     7501 catgccatac ttctctgggc cagtagcaga tgacctcata aaatatacta tattcaatag
     7561 ttctggtatt cagattggaa accacaatta tatggatgtt ggactgaatt cacaaccacc
     7621 aaacaatact tgcaaagaag agtcgacttc cagacaccaa gccatctttg ataacaccac
     7681 tagtctgact gatgaacacc tgaaccctat cagggaaaac ctgggaaggc agtggaaaaa
     7741 ctgtgcccgc aagctgggct tcactgagtc tcagatcgat gaaatcgacc atgactatga
     7801 aagagatgga ctgaaagaga aagtttacca aatgcttcag aagtggctga tgcgggaagg
     7861 caccaaaggg gccacagtgg gaaagttggc ccaggcactt caccaatgtt gcaggataga
     7921 cctgctgaac cacttgattc gtgccagcca gagctaagga tccgtcgacc tgcagccaag
     7981 ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa
     8041 aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttgttctt
     8101 gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat
     8161 tgaccacacc tctaccggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8221 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8281 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct
     8341 ttccagtcgg gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga
     8401 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8461 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8521 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8581 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8641 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8701 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8761 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8821 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8881 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8941 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9001 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9061 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9121 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9181 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9241 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9301 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9361 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9421 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9481 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9541 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9601 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9661 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9721 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9781 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9841 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9901 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9961 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10021 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10081 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10141 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10201 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10261 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10321 gttccgcgca catttccccg aaaagtgcca cct
//
