LOCUS       40924_4784        4307 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pAS104, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4307)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4307)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      complement(336..694)
                     /label=E35S
                     /note="E35S"
                     /regulatory_class="promoter"
     promoter        complement(764..782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(789..805)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      947..1327
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             1695..2486
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2507..3364
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3538..4126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc ggcttccttc aatttaaccg atgctaatga
      361 gttatttttg catgctttaa tttgtttcta tcaaatgttt attttttttt actagaaata
      421 acttattgca tttcattcaa aataagatca tacatacagg ttaaaataaa catagggaac
      481 ccaaatggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
      541 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcac
      601 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
      661 actgacgtaa gggatgacgc acaatcccac tatcaagccg aattctgcag atatccatca
      721 cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag tgagtcgtat
      781 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      841 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      901 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
      961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagagc tttacggcac
     1141 ctcgaccgca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     1201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1261 actggaacaa cactcaaccc tatcgcggtc tattcttttg atttataagg gattttgccg
     1321 atttcggcct attggttaaa aaatgagctg atttaacaaa ttcagggcgc aagggctgct
     1381 aaaggaaccg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     1441 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     1501 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     1561 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     1621 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     1681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     1741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     1801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     1861 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     1921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     1981 aagtgccggg gcaggatctc ctgtcatctc gccttgctcc tgccgagaaa gtatccatca
     2041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     2101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     2161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     2221 cgcgcatgcc cgacggcgag gatctcgtcg tgatccatgg cgatgcctgc ttgccgaata
     2281 tcatggtgga aaatggccgc ttttctggat tcaacgactg tggccggctg ggtgtggcgg
     2341 accgctatca ggacatagcg ttggataccc gtgatattgc tgaagagctt ggcggcgaat
     2401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     2461 tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga gtattcaaca
     2521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2701 aatgatgagc acttttaaag ttctgctatg tgatacacta ttatcccgta ttgacgccgg
     2761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3001 ggagctgaat gaagccatac caaacgacga gagtgacacc acgatgcctg tagcaatgcc
     3061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3121 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3181 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3241 agcactgggg ccagatggta agcgctcccg tatcgtagtt atctacacga cggggagtca
     3301 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3361 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3661 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4141 ggccttttta cggttcctgg gcttttgctg gccttttgct cacatgttct ttcctgcgtt
     4201 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4261 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
