LOCUS       40924_4779        5406 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Inducible expression vector pART3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Sandu C, Chiribau CB, Brandsch R.
  TITLE     Characterization of HdnoR, the transcriptional repressor of the 
            6-hydroxy-D-nicotine oxidase gene of Arthrobacter nicotinovorans 
            pAO1, and its DNA-binding activity in response to L- and D-nicotine 
            Derivatives
  JOURNAL   J. Biol. Chem. 278 (51), 51307-51315 (2003)
  PUBMED    14534317
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Sandu C, Chiribau CB, Sachelaru P, Brandsch R.
  TITLE     Plasmids for nicotine-dependent and -independent gene expression in 
            Arthrobacter nicotinovorans and other arthrobacter species
  JOURNAL   Appl. Environ. Microbiol. 71 (12), 8920-8924 (2005)
  PUBMED    16332890
REFERENCE   3  (bases 1 to 5406)
  AUTHORS   Sandu C, Brandsch R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2005) Molecular Biophysics, The Rockefeller 
            University, 1230 York Ave, Box 42, New York, NY 10021, USA
REFERENCE   4  (bases 1 to 5406)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2003"; volume: "278"; issue: "51"; pages: 
            "51307-51315"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "12"; 
            pages: "8920-8924"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (01-SEP-2005) Molecular Biophysics, The Rockefeller University, 1230
            York Ave, Box 42, New York, NY 10021, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..177
                     /note="PhdnO promoter from Arthrobacter nicotinovorans
                     pAO1"
                     /regulatory_class="promoter"
     repeat_region   10..33
                     /label=operator 2 (IR2)
                     /note="operator 2 (IR2)"
     repeat_region   84..120
                     /label=operator 1 (IR1)
                     /note="operator 1 (IR1)"
     regulatory      88..93
                     /regulatory_class="minus_35_signal"
     regulatory      110..116
                     /regulatory_class="minus_10_signal"
     misc_feature    176..178
                     /label=translation start codon
                     /note="translation start codon"
     misc_feature    178..231
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             232..255
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    256..258
                     /label=translation stop codon
                     /note="translation stop codon"
     polyA_signal    410..465
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(538..1126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1547..2359
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2893..3477
                     /codon_start=1
                     /gene="hnoR"
                     /product="HnoR"
                     /label=hnoR
                     /note="repressor of the 6-D-hydroxy-nicotine oxidase"
                     /protein_id="ABA41004.1"
                     /translation="MRISTVDRRQQLIDAAIRVIRRDGVESASLRTIASEAKASLAAVH
                     VCFTNKDELMQAAAAELLQQLVRSIPRVVDGSEDVRAIAHRVMDLFWAQMVSDELNILA
                     QFEIGIWAKRNPHHGDLSRTVYSDYEKEISKLLVAAAKRQKQTIAARNVARALIVIMDG
                     CSLQYFADPSDPRHKDLCDNLVDAYLDRVGL"
     gene            2893..3477
                     /gene="hnoR"
                     /label=hnoR
     rep_origin      3500..5406
                     /note="pCG100 origin of replication region from
                     Corynebacterium glutamicum"
ORIGIN
        1 ccatggtctt gacaaggaca agtgtccatg tcagggacaa agagcgatgt gttccggcct
       61 cccctagtcc cgcaaaaata acccccattg acatggacag ctgtccatgt atcaataggg
      121 tgatcactct ctcgaagacg agaatgctga ttgtcagcag acaaaggagt tggaaatgga
      181 tccgacgtcg tcgactttaa acctaggact agtggtaccc tgcagtctag acaccaccat
      241 caccatcacc atcactaagt gattaacctc aggtgcaggc tgcctatcag aaggtggtgg
      301 ctggtgtggc caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc
      361 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      421 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      721 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      961 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1141 cttttctacg gggtctgacg ctccatggaa tacgcgttta atgaccagca cagtcgtgat
     1201 ggcaaggtca gaatagcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca
     1261 ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt
     1321 gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt
     1381 gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag
     1441 ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1501 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1561 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1621 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     1681 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1741 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1801 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     1861 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1921 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1981 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2041 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     2101 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2161 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2221 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2281 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2341 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2401 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     2461 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     2521 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     2581 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     2641 cctcagcgct attctgacct tgccatcacg actgtgctgg tcattaaacg cgttaacccg
     2701 ggcccgcgga tgcatatgat cagatccgcc acctgggatg ccgcaccgcc tccgctacac
     2761 gccgaacgct tacttccctc aactgatccg cgttgctgtg aggccaaacc agaacggtcc
     2821 aaccgggcga ctaccctcat gggaccgcta cggcatttag agtagacctg agtccagggg
     2881 caaggagaca agttgcgtat ttccacggtg gatcgtcgcc aacagctcat cgacgcagct
     2941 attcgtgtaa ttaggcggga tggagtcgaa agtgccagcc taagaacgat agccagcgaa
     3001 gcaaaagctt ctctagcagc cgtgcacgtt tgcttcacga acaaagatga gctcatgcag
     3061 gcagctgccg cagagctgct tcagcaactg gtccggtcca ttccgcgcgt ggtcgacggc
     3121 tcggaggacg ttcgagctat cgcgcatcgg gtcatggact tgttttgggc ccagatggtt
     3181 tcagacgagt tgaatatcct ggcccaattt gaaataggga tctgggcgaa gcgtaacccg
     3241 catcacggtg acctgtcccg caccgtttac agcgactacg aaaaggaaat ctcaaaactg
     3301 ctggtcgccg ccgcaaagcg acagaagcag accatagctg ctcgaaacgt tgctagggcg
     3361 ctgattgtga tcatggatgg atgcagtctt cagtactttg ccgacccatc cgaccctcgc
     3421 cacaaggacc tctgcgacaa cctcgtagat gcatacctcg atcgggtagg actataaccc
     3481 ttgctcctta tggggatctt tgggagcagt ccttgtgcgc ttacgaggcg agccggtggg
     3541 gaacgttatc tgcctatggt gtgagccccc ctagagagct tcaagagcaa tcagcccgac
     3601 ctagaaagga ggccaagaga gagaccctac gggggaaccg ttttctgcct acgagatggc
     3661 acatttactg ggaagctttc ggcgtcctcg tggaagttca atgcccgcag accttaagtg
     3721 ctctattcac ggtctgacgt gacacgctaa attcagacat agcttcattg attgtcggcc
     3781 acgagccagt ctctccctca acagtcataa accaacctgc aatggtcaag cgatttcctt
     3841 tagctttcct agcttgtcgt tgactggact tagctagttt ttctcgctgt gctcgggcgt
     3901 actcactgtt tgggtctttc cagcgttctg cggccttttt accgccacgt cttcccatag
     3961 tggccagagc ttttcgcctc ggctggctct gcgtctctgt ctgacgagca gggacgactg
     4021 gctggccttt agcgacgtag ccgcgcacac gtcgcggcca atccgtctgg cggtcacagt
     4081 ccggcggcag atcaggctca cggcctcctc cgaccgcctg agcgacggtg taggcacgct
     4141 cgtaggctcg atgacccttg gtgtctttta ggcgctcacc agccgctttt aactggtatc
     4201 ccacagtcaa agcgtggcga aaagccgtct catcacgggc ggcacgccct ggagcagtcc
     4261 agaggacacg gacgccgtcg atcagctctc cagacgcttc agcggcgctc ggcaggcttg
     4321 cttcaagcgt ggcaagtgct tttgcttccg cagtggcttt tcttgccgct tcgatacgtg
     4381 cccgtccgct agaaaactcc tgctcatagc gttttttagg tttttctgtg cctgagatca
     4441 tgcgagcaac ctccataaga tcagctaggc gatccacgcg attgtgctgg gcatgccagc
     4501 ggtacggctg ggatcgtcgg agagtgcagt ggccacggct cagcctatgt gaaaaagcct
     4561 ggtcagcgcc gaaaacgcgg gtcatttcct cggtcgttgc agccagcagg cgcatattcg
     4621 ggctgctcat gcctgctgcg gcatacaccg gatcaatgag ccagatgagc tggcatttcc
     4681 cgctcagtgg attcacgccg atccaagctg gcgctttttc caggcgtgcc cagcgctcca
     4741 aaatcgcgta gacctcgggg tttacgtgct cgattttccc gccggcctgg tggctcggca
     4801 catcaatgtc caggacaagc acggctgcgt gctgcgcgtg cgtcagagca acatactggc
     4861 accgggcaag cgattttgaa ccaactcggt ataacttcgg ctgtgtttct ccgtgtccgg
     4921 gtctttgatc caagcgctgg cgaagtcgcg ggtcttgctg ccctggaaat tttctctgcc
     4981 caggtgagcg aggaattcgc ggcggtcttc gctcgtccag ccacgtgatc gcagcgcgag
     5041 ctcgggatgg gtgtcgaaca gatcagcgga aaatttccag gccggtgtgt caatgtctcg
     5101 tgaatccgct agagtcattt ttgagcgctt tctcccaggt ttggactggg ggttagccga
     5161 cgccctgtga gttaccgctc acggggcgtt caacattttt caggtattcg tgcagcttat
     5221 cgcttcttgc cgcctgtgcg ctttttcgac gcgcgacgct gctgccgatt cggtgcaggt
     5281 ggtggcggcg ctgacacgtc ctgggcggcc acggcacacg aaacgcggca tttacgatgt
     5341 ttgtcatgcc tgcgggcacc gccacgatcg cgataattct cgctgccgct tccagctctg
     5401 tgacga
//
