LOCUS       40924_4769        4634 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Constitutive expression vector pART2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Sandu C, Chiribau CB, Sachelaru P, Brandsch R.
  TITLE     Plasmids for nicotine-dependent and -independent gene expression in 
            Arthrobacter nicotinovorans and other arthrobacter species
  JOURNAL   Appl. Environ. Microbiol. 71 (12), 8920-8924 (2005)
  PUBMED    16332890
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Sandu C, Brandsch R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2005) Molecular Biophysics, The Rockefeller 
            University, 1230 York Ave, Box 42, New York, NY 10021, USA
REFERENCE   3  (bases 1 to 4634)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4634)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "12"; 
            pages: "8920-8924"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-SEP-2005) Molecular Biophysics, The Rockefeller University, 1230
            York Ave, Box 42, New York, NY 10021, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..177
                     /note="PhdnO promoter from Arthrobacter nicotinovorans
                     pAO1"
                     /regulatory_class="promoter"
     repeat_region   10..33
                     /label=operator 2 (IR2)
                     /note="operator 2 (IR2)"
     repeat_region   84..120
                     /label=operator 1 (IR1)
                     /rpt_family="inverted"
                     /note="operator 1 (IR1)"
     regulatory      88..93
                     /regulatory_class="minus_35_signal"
     regulatory      110..116
                     /regulatory_class="minus_10_signal"
     misc_feature    176..178
                     /label=translation start codon
                     /note="translation start codon"
     misc_feature    178..231
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             232..255
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    256..258
                     /label=translation stop codon
                     /note="translation stop codon"
     polyA_signal    410..465
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(538..1126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1547..2359
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2728..4634
                     /note="pCG100 origin of replication region from
                     Corynebacterium glutamicum"
ORIGIN
        1 ccatggtctt gacaaggaca agtgtccatg tcagggacaa agagcgatgt gttccggcct
       61 cccctagtcc cgcaaaaata acccccattg acatggacag ctgtccatgt atcaataggg
      121 tgatcactct ctcgaagacg agaatgctga ttgtcagcag acaaaggagt tggaaatgga
      181 tccgacgtcg tcgactttaa acctaggact agtggtaccc tgcagtctag acaccaccat
      241 caccatcacc atcactaagt gattaacctc aggtgcaggc tgcctatcag aaggtggtgg
      301 ctggtgtggc caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc
      361 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
      421 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
      481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      721 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      961 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1141 cttttctacg gggtctgacg ctccatggaa tacgcgttta atgaccagca cagtcgtgat
     1201 ggcaaggtca gaatagcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca
     1261 ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt
     1321 gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt
     1381 gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag
     1441 ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1501 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1561 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1621 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     1681 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1741 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1801 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     1861 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1921 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1981 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2041 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     2101 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2161 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2221 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2281 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2341 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2401 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     2461 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     2521 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     2581 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     2641 cctcagcgct attctgacct tgccatcacg actgtgctgg tcattaaacg cgttaacccg
     2701 ggcccgcgga tgcatatgat cagatctttg ggagcagtcc ttgtgcgctt acgaggcgag
     2761 ccggtgggga acgttatctg cctatggtgt gagcccccct agagagcttc aagagcaatc
     2821 agcccgacct agaaaggagg ccaagagaga gaccctacgg gggaaccgtt ttctgcctac
     2881 gagatggcac atttactggg aagctttcgg cgtcctcgtg gaagttcaat gcccgcagac
     2941 cttaagtgct ctattcacgg tctgacgtga cacgctaaat tcagacatag cttcattgat
     3001 tgtcggccac gagccagtct ctccctcaac agtcataaac caacctgcaa tggtcaagcg
     3061 atttccttta gctttcctag cttgtcgttg actggactta gctagttttt ctcgctgtgc
     3121 tcgggcgtac tcactgtttg ggtctttcca gcgttctgcg gcctttttac cgccacgtct
     3181 tcccatagtg gccagagctt ttcgcctcgg ctggctctgc gtctctgtct gacgagcagg
     3241 gacgactggc tggcctttag cgacgtagcc gcgcacacgt cgcggccaat ccgtctggcg
     3301 gtcacagtcc ggcggcagat caggctcacg gcctcctccg accgcctgag cgacggtgta
     3361 ggcacgctcg taggctcgat gacccttggt gtcttttagg cgctcaccag ccgcttttaa
     3421 ctggtatccc acagtcaaag cgtggcgaaa agccgtctca tcacgggcgg cacgccctgg
     3481 agcagtccag aggacacgga cgccgtcgat cagctctcca gacgcttcag cggcgctcgg
     3541 caggcttgct tcaagcgtgg caagtgcttt tgcttccgca gtggcttttc ttgccgcttc
     3601 gatacgtgcc cgtccgctag aaaactcctg ctcatagcgt tttttaggtt tttctgtgcc
     3661 tgagatcatg cgagcaacct ccataagatc agctaggcga tccacgcgat tgtgctgggc
     3721 atgccagcgg tacggctggg atcgtcggag agtgcagtgg ccacggctca gcctatgtga
     3781 aaaagcctgg tcagcgccga aaacgcgggt catttcctcg gtcgttgcag ccagcaggcg
     3841 catattcggg ctgctcatgc ctgctgcggc atacaccgga tcaatgagcc agatgagctg
     3901 gcatttcccg ctcagtggat tcacgccgat ccaagctggc gctttttcca ggcgtgccca
     3961 gcgctccaaa atcgcgtaga cctcggggtt tacgtgctcg attttcccgc cggcctggtg
     4021 gctcggcaca tcaatgtcca ggacaagcac ggctgcgtgc tgcgcgtgcg tcagagcaac
     4081 atactggcac cgggcaagcg attttgaacc aactcggtat aacttcggct gtgtttctcc
     4141 gtgtccgggt ctttgatcca agcgctggcg aagtcgcggg tcttgctgcc ctggaaattt
     4201 tctctgccca ggtgagcgag gaattcgcgg cggtcttcgc tcgtccagcc acgtgatcgc
     4261 agcgcgagct cgggatgggt gtcgaacaga tcagcggaaa atttccaggc cggtgtgtca
     4321 atgtctcgtg aatccgctag agtcattttt gagcgctttc tcccaggttt ggactggggg
     4381 ttagccgacg ccctgtgagt taccgctcac ggggcgttca acatttttca ggtattcgtg
     4441 cagcttatcg cttcttgccg cctgtgcgct ttttcgacgc gcgacgctgc tgccgattcg
     4501 gtgcaggtgg tggcggcgct gacacgtcct gggcggccac ggcacacgaa acgcggcatt
     4561 tacgatgttt gtcatgcctg cgggcaccgc cacgatcgcg ataattctcg ctgccgcttc
     4621 cagctctgtg acga
//
