LOCUS       40924_4764        7737 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pARS3m, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) Crop Improvement and Genetics Research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   2  (bases 1 to 7737)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7737)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-OCT-2015) Crop Improvement and Genetics Research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    68..235
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        236..1944
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin
                     gene RUBQ2"
     CDS             1945..2172
                     /codon_start=1
                     /label=ubiquitin
                     /note="Saccharomyces cerevisiae ubiquitin"
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLADYNIQKESTLHLVLRLRGG"
     CDS             2179..3201
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    3223..3397
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(3510..3534)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      3612..4243
                     /label=oriV
                     /note="incP origin of replication"
     CDS             4455..5246
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             5548..6693
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(6936..7524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagtttc gcgtagcgga taacaatttc
       61 acacagggat atcactagta aaaggtacca gtttaaacac gcgcgcgagg cgcgccacct
      121 gcaggagagc tcctgcagta tcgatgcggc cgcaaagtcg acgaattctc ccgggtggat
      181 ccaaggcctt ctagaaaaac tcgagaacgt tgggccccaa ttggctagca agcttattcg
      241 ggtcaagggg aagccagcgc gccaccccac gtcagcaaat acggaggcgc ggggttgacg
      301 gcgtcacccg gtcctaacgg cgaccaacaa accagccaga agaaattaca gtaaaaaaaa
      361 agtaaattgc actttgatcc accttttatt acctaagtct caatttggat cacccttaaa
      421 cctatctttt caatttgggc cgggttgtgg tttggactac catgaacaac ttttcgtcat
      481 gtctaacttc cctttcagca aacatatgaa ccatatatag aggagatcgg ccgtatacta
      541 gagctgatgt gtttaaggtc gttgattgca cgagaaaaaa aaatccaaat cgcaacaata
      601 gcaaatttat ctggttcaaa gtgaaaagat atgtttaaag gtagtccaaa gtaaaactta
      661 tagataataa aatgtggtcc aaagcgtaat tcactcaaaa aaaatcaacg agacgtgtac
      721 caaacggaga caaacggcat cttctcgaaa tttcccaacc gctcgctcgc ccgcctcgtc
      781 ttcccggaaa ccgcggtggt ttcagcgtgg cggattctcc aagcagacgg agacgtcacg
      841 gcacgggact cctcccacca cccaaccgcc ataaatacca gccccctcat ctcctctcct
      901 cgcatcagct ccacccccga aaaatttctc cccaatctcg cgaggctctc gtcgtcgaat
      961 cgaatcctct cgcgtcctca aggtacgctg cttctcctct cctcgcttcg tttcgattcg
     1021 atttcggacg ggtgaggttg ttttgttgct agatccgatt ggtggttagg gttgtcgatg
     1081 tgattatcgt gagatgttta ggggttgtag atctgatggt tgtgatttgg gcacggttgg
     1141 ttcgataggt ggaatcgtgg ttaggttttg ggattggatg ttggttctga tgattggggg
     1201 gaatttttac ggttagatga attgttggat gattcgattg gggaaatcgg tgtagatctg
     1261 ttggggaatt gtggaactag tcatgcctga gtgattggtg cgatttgtag cgtgttccat
     1321 cttgtaggcc ttgttgcgag catgttcaga tctactgttc cgctcttgat tgagttattg
     1381 gtgccttggg ttggtgcaaa cacaggcttt aatatgttat atctgttttg tgtttgatgt
     1441 agatctgtag ggtagttctt cttagacatg gttcaattat gtagcttgtg cgtttcgatt
     1501 tgatttcata tgttcacaga ttagataatg atgaactctt ttaattaatt gtcaatggta
     1561 aataggaagt cttgtcgcta tatctgtcat aatgatctca tgttactatc tgccagtaat
     1621 ttatgctaag aactatatta gaatatcatg ttacaatctg tagtaatatc atgttacaat
     1681 ctgtagttca tctatataat ctattgtggt aatttctttt tactatctgt gtgaagatta
     1741 ttgccactag ttcattctac ttatttctga agttcaggat acgtgtgctg ttactaccta
     1801 tctgaataca tgtgtgatgt gcctgttact atctttttga atacatgtat gttctgttgg
     1861 aatatgtttg ctgtttgatc cgttgttgtg tccttaatct tgtgctagtt cttaccctat
     1921 ctgtttggtg attatttctt gcagatgcag atctttgtga agacattgac cggcaagact
     1981 atcaccctgg aggtggagtc ctctgacacc atcgacaatg tcaaggctaa gatccaagat
     2041 aaggagggca tccccccgga ccagcagcgt ctcatcttcg ctggcaagca gctcgaagat
     2101 ggcaggaccc ttgctgacta caacatccag aaggagtcca cccttcacct tgtcctccgc
     2161 ctccgtggtg gcggatccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     2221 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     2281 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     2341 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     2401 ccggaagtgc ttgacattgg ggagtttagc gagagcctga cctattgcat ctcccgccgt
     2461 tcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg
     2521 gtcgcggagg ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     2581 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     2641 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     2701 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     2761 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     2821 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     2881 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     2941 gagcttgcag gatcgccacg actccgggcg tatatgctcc gcattggtct tgaccaactc
     3001 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     3061 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     3121 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     3181 actcgtccga gggcaaagaa atagttaatc gacaagctcg agtttctcca taataatgtg
     3241 tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca
     3301 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     3361 attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt cggcgttaat
     3421 tcagtacatt aaaaacgcgt acggttaaaa ccaccccagt acattaaaaa cgtccgcaat
     3481 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     3541 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     3601 tcagtccact agacgctcac cgggctggtt gccctcgccg ctgggctggc ggccgtctat
     3661 ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg cagccgccgg
     3721 cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc ggacgttgac
     3781 acttgagggg ccgactcacc cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg
     3841 ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt
     3901 ttcccacaga tgatgtggac aagcctgggg ataagtgccc tgcggtattg acacttgagg
     3961 ggcgcgacta ctgacagatg aggggcgcga tccttgacac ttgaggggca gagtgctgac
     4021 agatgagggg cgcacctatt gacatttgag gggctgtcca caggcagaaa atccagcatt
     4081 tgcaagggtt tccgcccgtt tttcggccac cgctaacctg tcttttaacc tgcttttaaa
     4141 ccaatattta taaaccttgt ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg
     4201 ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc gctctcgcgt tggcagcatc
     4261 acccataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     4321 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     4381 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     4441 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     4501 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     4561 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     4621 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     4681 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     4741 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     4801 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     4861 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     4921 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     4981 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     5041 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     5101 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatt ggggaagaac
     5161 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     5221 attatatttt actggatgaa ttgttttagt acctagatgt ggcgcaacga tgccggcgac
     5281 aagcaggagc gcaccgactt cttccgcatc aagtgttttg gctctcaggc cgaggcccac
     5341 ggcaagtatt tgggcaaggg gtcgctggta ttcgtgcagg gcaagattcg gaataccaag
     5401 tacgagaagg acggccagac ggtctacggg accgacttca ttgccgataa ggtggattat
     5461 ctggacacca aggcaccagg cgggtcaaat caggaataag ggcacattgc cccggcgtga
     5521 gtcggggcaa tcccgcaagg agggtgaatg aatcggacgt ttgaccggaa ggcatacagg
     5581 caagaactga tcgacgcggg gttttccgcc gaggatgccg aaaccatcgc aagccgcacc
     5641 gtcatgcgtg cgccccgcga aaccttccag tccgtcggct cgatggtcca gcaagctacg
     5701 gccaagatcg agcgcgacag cgtgcaactg gctccccctg ccctgcccgc gccatcggcc
     5761 gccgtggagc gttcgcgtcg tctcgaacag gaggcggcag gtttggcgaa gtcgatgacc
     5821 atcgacacgc gaggaactat gacgaccaag aagcgaaaaa ccgccggcga ggacctggca
     5881 aaacaggtca gcgaggccaa gcaagccgcg ttgctgaaac acacgaagca gcagatcaag
     5941 gaaatgcagc tttccttgtt cgatattgcg ccgtggccgg acacgatgcg agcgatgcca
     6001 aacgacacgg cccgctctgc cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg
     6061 ctgcaaaaca aggtcatttt ccacgtcaac aaggacgtga agatcaccta caccggcgtc
     6121 gagctgcggg ccgacgatga cgaactggtg tggcagcagg tgttggagta cgcgaagcgc
     6181 acccctatcg gcgagccgat caccttcacg ttctacgagc tttgccagga cctgggctgg
     6241 tcgatcaatg gccggtatta cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg
     6301 gcgatgggct tcacgtccga ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc
     6361 ttccgcgtcc tggaccgtgg caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa
     6421 atcgtcgtgc tgtttgctgg cgaccactac acgaaattca tatgggagaa gtaccgcaag
     6481 ctgtcgccga cggcccgacg gatgttcgac tatttcagct cgcaccggga gccgtacccg
     6541 ctcaagctgg aaaccttccg cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg
     6601 cgcgagcagg tcggcgaagc ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc
     6661 tgggtcaatg atgacctggt gcattgcaaa cgctagggcc ttgtggggtc agttccggct
     6721 gggggttcag cagccagcgc tttactgaga tcctcttccg cttcctcgct cactgactcg
     6781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7621 acctagatcc ttttggatct cctgtggttg gcatgcacat acaaatggac gaacggataa
     7681 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttag
//
