LOCUS       40924_4714        6144 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARAn58, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Roth AH, Dersch P.
  TITLE     A novel expression system for intracellular production and 
            purification of recombinant affinity-tagged proteins in Aspergillus 
            niger
  JOURNAL   Appl. Microbiol. Biotechnol. 86 (2), 659-670 (2010)
  PUBMED    19908039
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Dersch P, Roth A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2009) Institute of Microbiology, Technische 
            Universitat Braunschweig, Spielmannstr. 7, Braunschweig 38106, 
            Germany
REFERENCE   3  (bases 1 to 6144)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2010"; volume: "86"; issue: "2"; 
            pages: "659-670"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2009) Institute of Microbiology, Technische Universitat 
            Braunschweig, Spielmannstr. 7, Braunschweig 38106, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1194
                     /label=suc1
                     /note="suc1"
                     /regulatory_class="promoter"
     regulatory      1245..2313
                     /label=gla
                     /note="gla"
                     /regulatory_class="terminator"
     primer_bind     complement(2345..2361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2831..2935
                     /label=AmpR promoter
     CDS             2936..3793
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3967..4555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             join(5217..5373,5433..6100)
                     /codon_start=1
                     /gene="pyrG"
                     /product="orotidine-5'-phosphate decarboxylase"
                     /label=pyrG
                     /protein_id="ACP41233.1"
                     /translation="MSSKSHLPYAIRATNHPNPLTSKLFSIAEEKKTNVTVSADVTTSA
                     ELLDLADRLGPYIAVLKTHIDILTDLTPSTLSSLQSLATKHNFLIFEDRKFIDIGNTVQ
                     KQYHGGALRISEWAHIINCAILPGEGIVEALAQTTKSPDFKDANQRGLLILAEMTSKGS
                     LATGEYTARSVEYARKYKGFVMGFVSTRALSEVLPEQKEESEDFVVFTTGVNLSDKGDK
                     LGQQYQTPGSAVGRGADFIIAGRGIYKADDPVEAVQRYREEGWKAYEKRVGL"
     gene            5217..6100
                     /gene="pyrG"
                     /label=pyrG
ORIGIN
        1 agtagtgata tgtcggctct agttccttgc tgtaagtaag gagacgggta tatgtcaacg
       61 tagtataaag tgaagcaggc gaattttaaa gaatttgcaa tggcccatct gtgttccatc
      121 ttcgcacaac atttaattat tttatatgat ttgtcataga cccaccaact tcaactcgaa
      181 ctacatcact tggaccaaca tcacgcgttc aacatggaca agggttgata acccgggatg
      241 cattcattgt ctgcctcaag tgtcgctact agttccatct tttgtaaatt tggatatgtc
      301 cgttttcttc tatcgcaaca agccatccaa caagagaata ccgacctgga tcatgtaatt
      361 ctggcaatgg tcaacagccc cgtcccgcag agcatacgga ttcttgtttt gcgacccccc
      421 tggaacgata taataatttc gcagtctcca ctatattccg gcccgggaaa cacccttggg
      481 tcctctctag gcacgactga ttgataatga tatcgtaaga agtacaacct tgggctgagg
      541 gcctgccacc catcttccat gataagacat ctttctggac gatcagcgct gtcacaggtg
      601 acgggccagt gtgaacaaat cacatcgaag gcacacattg cgcgatgaag actctgcact
      661 ctattgcgtc aaacacgcaa accattatac ctgaagcatc acctcgtgtc ctatacgagt
      721 cgttaaactt ttcttcctct attctgccca cttttcattt cttttatact cccgttcttg
      781 ctcgatctcc accgcttcat accgacaaac ctccggcttt cagtttcttc ttacggtaaa
      841 accaaagcac cccacgagca agctagattc cacaccaggt aacttcagtc aagccaacag
      901 aacctcggtg ggtctcaagt tgaagatcgg cagcatcata acctcgttgc gagaactcgg
      961 gggaaatctc ggggaatccg cgctgaatct gcccccgcga accctaagcg gacttcctcc
     1021 gcaatcctca atttccccga ggatacggac tattcatcca gcattccaac cattctagtt
     1081 ctagtgatcg gaaattcccc ggcacccctg catcaacaga agcataccat atataatgtc
     1141 acaatttccc ctctgccaag ggaggggaag tgagtgtccc ctcctctgct attgctagcc
     1201 ttaagatcga taccggttgt acagcgcgcg aattctaagg ccggcccgac cgcgacggtg
     1261 actgacacct ggcggtagac aatcaatcca tttcgctata gttaaaggat ggggatgagg
     1321 gcaattggtt atatgatcat gtatgtagtg ggtgtgcata atagtagtga aatggaagcc
     1381 aagtcatgtg attgtaatcg accgacggaa ttgaggatat ccggaaatac agacaccgtg
     1441 aaagccatgg tcttcccttc gtgtagaaga ccagacagac agtccctgat ttacccttgc
     1501 acaaagcact agaaaattag cattccatcc ttctctgctt gctctgctga tatcactgtc
     1561 attcaatgca tagccatgag ctcatcttag atccaagcac gtaattccat agccgaggtc
     1621 cacagtggag cagcaacatt ccccatcatt gctttcccca ggggcctccc aacgactaaa
     1681 tcaagagtat atctctaccg tccaatagat cgtcttcgct tcaaaatctt tgacaattcc
     1741 aagagggtcc ccatccatca aacccagttc aataatagcc gagatgcatg gtggagtcaa
     1801 ttaggcagta ttgctggaat gtcggggcca gttggcccgg tggtcattgg ccgcctgtga
     1861 tgccatctgc cactaaatcc gatcattgat ccaccgccca cgaggcgcgt ctttgctttt
     1921 tgcgcggcgt ccaggttcaa ctctctctgc agctccagtc caacgctgac tgactagttt
     1981 acctactggt ctgatcggct ccatcagagc tatggcgtta tcccgtgccg ttgctgcgca
     2041 atcgctatct tgatcgcaac cttgaactca ctcttgtttt aatagtgatc ttggtgacgg
     2101 agtgtcggtg agtgacaacc aacatcgtgc aagggagatt gatacggaat tgtcgctccc
     2161 atcatgatgt tcttgccggc tttgttggcc ctattcgtgg gatgcgatgc cctcgctgtg
     2221 cagcagcagg tactgctgga tgaggagcca tcggtctctg cacgcaaacc caacttcctc
     2281 ttcattctca cggatgatca ggatctccgg aatattcgcg gacctgcagg catgcaagct
     2341 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2401 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2461 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     2521 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     2581 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2641 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2701 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2761 gcctattttt ataggttaat gtcatgataa taatggtttc ttagcaggtg gcacttttcg
     2821 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2881 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2941 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3001 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3061 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3121 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3181 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3241 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     3301 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     3361 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3421 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3481 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3541 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3601 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3661 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3721 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3781 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3841 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3901 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3961 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4021 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4081 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4141 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4201 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4261 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4321 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4381 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4441 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4501 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4561 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4621 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4681 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag ctgcggccgc
     4741 gcaacttcct cgagaacgcg ccgcagacaa tgctctctat cctggtggca ggcgtcaagt
     4801 acccagaggc agcagcgggc ttaggagcgg cctgggttgt tctccgcacc ctctacatgc
     4861 tgggctatat ttatagcgac aagccgaacg gcaccggcag gtacaatggt tcgctgtact
     4921 tgcttgcgca agcgggtctt tggggattga gcgcatttgg tgttgcaaag gatttgatgt
     4981 aaatgtagtc gacatcttag cacagagggg agagttgata aaatgtggtc tgtttgaatg
     5041 atagtcgggt tcgtgaccta tattcgtgat agtggagata ggtctgcgcc tatcttatcg
     5101 ggccggagca aaaattccac cgcagcgggg tgagttttcg ttatacagcc atcccacttc
     5161 cagcttcaaa ttgtcagttt aatccagccc aattcaatca ttggagaacc gccatcatgt
     5221 cttcgaagtc ccacctcccc tacgcaattc gcgcaaccaa ccatcccaac cctttaacat
     5281 ctaaactctt ctccatcgcc gaggagaaga aaaccaacgt caccgtctcc gcagacgtta
     5341 ctacttccgc cgagctcctc gatcttgctg accgtacatc ctgcaccaat gcccctccag
     5401 gataacaaat agctgatgcg tagtgagtac aggcctaggc ccctatatcg cagttctgaa
     5461 aacccacatc gacatcctca ccgatctcac cccgtcgacc ctttcctcgc tccaatccct
     5521 cgcgacaaag cacaacttcc tcatctttga ggaccgcaag ttcatcgaca tcggcaacac
     5581 cgtgcaaaag cagtaccacg gtggcgctct ccgcatctcc gaatgggcac acatcatcaa
     5641 ctgcgccatc ctgccgggcg aagggatcgt cgaggccctc gcacagacaa ccaagtctcc
     5701 tgactttaaa gacgcgaatc aacgaggtct cctgattctt gccgagatga cgagtaaggg
     5761 atctcttgcg acaggggagt acacggcacg ctcggttgag tacgcgcgga agtataaggg
     5821 gtttgtgatg ggattcgtga gtacaagggc gttgagtgag gtgctgcccg aacagaaaga
     5881 ggagagcgag gattttgtcg tctttacgac tggggtgaat ctgtcggata agggggataa
     5941 gctggggcag cagtatcaga cacctgggtc ggcggttggg cgaggtgcgg actttatcat
     6001 tgcgggtagg ggcatctata aggcggacga tccagtcgag gcggttcaga ggtaccggga
     6061 ggaaggctgg aaagcttacg agaaaagagt tggactttga gtgtgagtgg aaatgtgtaa
     6121 cggtattgac taaaagggga cgtc
//
