LOCUS       40924_4709        6125 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARAn51, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Roth AH, Dersch P.
  TITLE     A novel expression system for intracellular production and 
            purification of recombinant affinity-tagged proteins in Aspergillus 
            niger
  JOURNAL   Appl. Microbiol. Biotechnol. 86 (2), 659-670 (2010)
  PUBMED    19908039
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Dersch P, Roth A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2009) Institute of Microbiology, Technische 
            Universitat Braunschweig, Spielmannstr. 7, Braunschweig 38106, 
            Germany
REFERENCE   3  (bases 1 to 6125)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2010"; volume: "86"; issue: "2"; 
            pages: "659-670"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAR-2009) Institute of Microbiology, Technische Universitat 
            Braunschweig, Spielmannstr. 7, Braunschweig 38106, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      142..1175
                     /label=pki
                     /note="pki"
                     /regulatory_class="promoter"
     regulatory      1226..2294
                     /label=gla
                     /note="gla"
                     /regulatory_class="terminator"
     primer_bind     complement(2326..2342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2812..2916
                     /label=AmpR promoter
     CDS             2917..3774
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3948..4536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             join(5198..5354,5414..6081)
                     /codon_start=1
                     /gene="pyrG"
                     /product="orotidine-5'-phosphate decarboxylase"
                     /label=pyrG
                     /protein_id="ACP41235.1"
                     /translation="MSSKSHLPYAIRATNHPNPLTSKLFSIAEEKKTNVTVSADVTTSA
                     ELLDLADRLGPYIAVLKTHIDILTDLTPSTLSSLQSLATKHNFLIFEDRKFIDIGNTVQ
                     KQYHGGALRISEWAHIINCAILPGEGIVEALAQTTKSPDFKDANQRGLLILAEMTSKGS
                     LATGEYTARSVEYARKYKGFVMGFVSTRALSEVLPEQKEESEDFVVFTTGVNLSDKGDK
                     LGQQYQTPGSAVGRGADFIIAGRGIYKADDPVEAVQRYREEGWKAYEKRVGL"
     gene            5198..6081
                     /gene="pyrG"
                     /label=pyrG
ORIGIN
        1 ccacatctga tgccattggc ggaggggtcc ggacggtcag gaacttagcc ttatgagatg
       61 aatgatggac gtgtctggcc tcggaaaagg atatatgggg atcataatag tactagccat
      121 attaatgaag ggcatatacc acgcgtaaat ggaagagaaa acctccgagt acttacttag
      181 ggccctgtct actggccaga gtctcgtcct ctattcacta tgataaatta cccactggac
      241 aaaaaaataa aataaaataa aaataaaaag ggagacagct tctccatcaa ctggcaactg
      301 ggtccgtccg agcagagcaa aattcagcct tatgggttcc gatggagtca gggaaatagt
      361 tcttgcgaag ggcattgggc ttttttgcga ggagaaaatt cagcaccgac aaagcatccg
      421 aaatccgacc tcgctaggag agaatggatc cgcgacgatg tggggtcaac tggacagagt
      481 gagagggtat catgtggtcc tgccagatac ttcgcagaat gttgtgtggg tgtctgattg
      541 tggcttgggc gtgaattgct tttggtcttc ccaaccaatt attattgcat gcggcgtatg
      601 aatgcctgag atgcgcggag ggaaggtgcc tgaggatgta gtggacaaat gctgctgatc
      661 gctgggcgga aacccttggc tgaccagtga aaagagcgga cggaggcagc aggtgtatct
      721 acgatcaaag aatagtagca aagcagtgaa aggtggatca cccagcaaat aattgagttt
      781 tgatacccag cgatagtgcc gggggggaga aaaagtcatt aataatggga attatgtagg
      841 cgatgggaag tgtgattgta actactccgt agctggaggc acaactaaca agccagctct
      901 caacccgcgg ggaaccgacc gacagaaaaa agcgtcccaa agcaggaatc ccaccaaaaa
      961 gggccgatcc agccaatcac cgccgccaac atttttcctt cccgggcacc cctcctctag
     1021 tccaccatct ctctcttctc tcgctcaccg gccccgtctt ttccttccct attatctctc
     1081 cctcttctcc tcccttctct ccctccattc tttctcccat cttcatcaat cccttctctt
     1141 ctgtcttccc ccccggttca gtagagatca atcagctagc cttaagatcg ataccggttg
     1201 tacagcgcgc gaattctaag gccggcccga ccgcgacggt gactgacacc tggcggtaga
     1261 caatcaatcc atttcgctat agttaaagga tggggatgag ggcaattggt tatatgatca
     1321 tgtatgtagt gggtgtgcat aatagtagtg aaatggaagc caagtcatgt gattgtaatc
     1381 gaccgacgga attgaggata tccggaaata cagacaccgt gaaagccatg gtcttccctt
     1441 cgtgtagaag accagacaga cagtccctga tttacccttg cacaaagcac tagaaaatta
     1501 gcattccatc cttctctgct tgctctgctg atatcactgt cattcaatgc atagccatga
     1561 gctcatctta gatccaagca cgtaattcca tagccgaggt ccacagtgga gcagcaacat
     1621 tccccatcat tgctttcccc aggggcctcc caacgactaa atcaagagta tatctctacc
     1681 gtccaataga tcgtcttcgc ttcaaaatct ttgacaattc caagagggtc cccatccatc
     1741 aaacccagtt caataatagc cgagatgcat ggtggagtca attaggcagt attgctggaa
     1801 tgtcggggcc agttggcccg gtggtcattg gccgcctgtg atgccatctg ccactaaatc
     1861 cgatcattga tccaccgccc acgaggcgcg tctttgcttt ttgcgcggcg tccaggttca
     1921 actctctctg cagctccagt ccaacgctga ctgactagtt tacctactgg tctgatcggc
     1981 tccatcagag ctatggcgtt atcccgtgcc gttgctgcgc aatcgctatc ttgatcgcaa
     2041 ccttgaactc actcttgttt taatagtgat cttggtgacg gagtgtcggt gagtgacaac
     2101 caacatcgtg caagggagat tgatacggaa ttgtcgctcc catcatgatg ttcttgccgg
     2161 ctttgttggc cctattcgtg ggatgcgatg ccctcgctgt gcagcagcag gtactgctgg
     2221 atgaggagcc atcggtctct gcacgcaaac ccaacttcct cttcattctc acggatgatc
     2281 aggatctccg gaatattcgc ggacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     2341 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2401 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2461 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2521 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2581 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2641 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2701 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2761 tgtcatgata ataatggttt cttagcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2821 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2881 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2941 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3001 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3061 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3121 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3181 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3241 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3301 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3361 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3421 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3481 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3541 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3601 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4561 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4621 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4681 accgagcgca gcgagtcagt gagcgaggaa gctgcggccg cgcaacttcc tcgagaacgc
     4741 gccgcagaca atgctctcta tcctggtggc aggcgtcaag tacccagagg cagcagcggg
     4801 cttaggagcg gcctgggttg ttctccgcac cctctacatg ctgggctata tttatagcga
     4861 caagccgaac ggcaccggca ggtacaatgg ttcgctgtac ttgcttgcgc aagcgggtct
     4921 ttggggattg agcgcatttg gtgttgcaaa ggatttgatg taaatgtagt cgacatctta
     4981 gcacagaggg gagagttgat aaaatgtggt ctgtttgaat gatagtcggg ttcgtgacct
     5041 atattcgtga tagtggagat aggtctgcgc ctatcttatc gggccggagc aaaaattcca
     5101 ccgcagcggg gtgagttttc gttatacagc catcccactt ccagcttcaa attgtcagtt
     5161 taatccagcc caattcaatc attggagaac cgccatcatg tcttcgaagt cccacctccc
     5221 ctacgcaatt cgcgcaacca accatcccaa ccctttaaca tctaaactct tctccatcgc
     5281 cgaggagaag aaaaccaacg tcaccgtctc cgcagacgtt actacttccg ccgagctcct
     5341 cgatcttgct gaccgtacat cctgcaccaa tgcccctcca ggataacaaa tagctgatgc
     5401 gtagtgagta caggcctagg cccctatatc gcagttctga aaacccacat cgacatcctc
     5461 accgatctca ccccgtcgac cctttcctcg ctccaatccc tcgcgacaaa gcacaacttc
     5521 ctcatctttg aggaccgcaa gttcatcgac atcggcaaca ccgtgcaaaa gcagtaccac
     5581 ggtggcgctc tccgcatctc cgaatgggca cacatcatca actgcgccat cctgccgggc
     5641 gaagggatcg tcgaggccct cgcacagaca accaagtctc ctgactttaa agacgcgaat
     5701 caacgaggtc tcctgattct tgccgagatg acgagtaagg gatctcttgc gacaggggag
     5761 tacacggcac gctcggttga gtacgcgcgg aagtataagg ggtttgtgat gggattcgtg
     5821 agtacaaggg cgttgagtga ggtgctgccc gaacagaaag aggagagcga ggattttgtc
     5881 gtctttacga ctggggtgaa tctgtcggat aagggggata agctggggca gcagtatcag
     5941 acacctgggt cggcggttgg gcgaggtgcg gactttatca ttgcgggtag gggcatctat
     6001 aaggcggacg atccagtcga ggcggttcag aggtaccggg aggaaggctg gaaagcttac
     6061 gagaaaagag ttggactttg agtgtgagtg gaaatgtgta acggtattga ctaaaagggg
     6121 acgtc
//
