LOCUS       40924_4684       10530 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pAPn, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Zare B, Malboobi MA, Sabet MS, Norouzi P.
  TITLE     High plant transformation efficiency using a new chimeric P-DNA 
            derived from sugar beet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   Zare B, Malboobi MA, Sabet MS, Norouzi P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2015) Plant Biotechnology, NIGEB, Pajoohesh Blvd.,
            Tehran 009821, Iran
REFERENCE   3  (bases 1 to 10530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2015) Plant Biotechnology, NIGEB, Pajoohesh Blvd., Tehran 
            009821, Iran"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            140..249
                     /label=oriT
                     /note="incP origin of transfer"
     mobile_element  309..1076
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             1076..1426
                     /label=traJ
                     /note="oriT-recognizing protein"
     CDS             1701..2846
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(4209..4797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5894..6682
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      complement(7287..7999)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    7999..8307
                     /label=PB, a new chimeric P-DNA derived from sugar beet
                     /note="PB, a new chimeric P-DNA derived from sugar beet"
     misc_feature    7999..8112
                     /label=ACR region of pTiC58
                     /note="ACR region of pTiC58"
     promoter        complement(8347..8365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8372..8388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8546..8729
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8730..9548
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     regulatory      9552..10393
                     /gene="nos"
                     /regulatory_class="terminator"
     gene            9552..10393
                     /gene="nos"
                     /label=nos
                     /note="nopaline synthase"
     misc_feature    10394..10487
                     /label=PB, a new chimeric P-DNA derived from sugar beet
                     /note="PB, a new chimeric P-DNA derived from sugar beet"
     misc_feature    10488..10530
                     /label=C-clusters region of pTiC58
                     /note="C-clusters region of pTiC58"
ORIGIN
        1 ggatcttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
       61 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
      121 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
      181 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
      241 gctggccggc taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc
      301 caaccagggg tgatgctgcc aacttactga tttagtgtat gatggtgttt ttgaggtgct
      361 ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt
      421 aacggcaaaa gcaccgccgg acatcagcgc tatctctgct ctcactgccg taaaacatgg
      481 caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat cattgatatg
      541 gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg
      601 attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata cagccgggca
      661 gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc
      721 gccagcgctg gctgttttac gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat
      781 tcggtgaacg cactatggcg acgctggggc gtcttatgag cctgctgtca ccctttgacg
      841 tggtgatatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc
      901 acgtaatcag caagcgatat acgcagcgaa ttgagcggca taacctgaat ctgaggcagc
      961 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
     1021 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccaac
     1081 caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga
     1141 ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg tcggccaggg
     1201 ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg
     1261 cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc
     1321 gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga
     1381 gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc
     1441 gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga
     1501 agagcgactt cgcggagctg gtattcgtgc agggcaagat tcggaatacc aagtacgaga
     1561 aggacggcca gacggtctac gggaccgact tcattgccga taaggtggat tatctggaca
     1621 ccaaggcacc aggcgggtca aatcaggaat aagggcacat tgccccggcg tgagtcgggg
     1681 caatcccgca aggagggtga atgaatcgga cgtttgaccg gaaggcatac aggcaagaac
     1741 tgatcgacgc ggggttttcc gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc
     1801 gtgcgccccg cgaaaccttc cagtccgtcg gctcgatggt ccagcaagct acggccaaga
     1861 tcgagcgcga cagcgtgcaa ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg
     1921 agcgttcgcg tcgtctcgaa caggaggcgg caggtttggc gaagtcgatg accatcgaca
     1981 cgcgaggaac tatgacgacc aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg
     2041 tcagcgaggc caagcaggcc gcgttgctga aacacacgaa gcagcagatc aaggaaatgc
     2101 agctttcctt gttcgatatt gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca
     2161 cggcccgctc tgccctgttc accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa
     2221 acaaggtcat tttccacgtc aacaaggacg tgaagatcac ctacaccggc gtcgagctgc
     2281 gggccgacga tgacgaactg gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta
     2341 tcggcgagcc gatcaccttc acgttctacg agctttgcca ggacctgggc tggtcgatca
     2401 atggccggta ttacacgaag gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg
     2461 gcttcacgtc cgaccgcgtt gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg
     2521 tcctggaccg tggcaagaaa acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg
     2581 tgctgtttgc tggcgaccac tacacgaaat tcatatggga gaagtaccgc aagctgtcgc
     2641 cgacggcccg acggatgttc gactatttca gctcgcaccg ggagccgtac ccgctcaagc
     2701 tggaaacctt ccgcctcatg tgcggatcgg attccacccg cgtgaagaag tggcgcgagc
     2761 aggtcggcga agcctgcgaa gagttgcgag gcagcggcct ggtggaacac gcctgggtca
     2821 atgatgacct ggtgcattgc aaacgctagg gccttgtggg gtcagttccg gctgggggtt
     2881 cagcagccag cgctttactg gcatttcagg aacaagcggg cactgctcga cgcacttgct
     2941 tcgctcagta tcgctcggga cgcacggcgc gctctacgaa ctgccgataa acagaggatt
     3001 aaaattgaca attgtgatta aggctcagat tcgacggctt ggagcggccg acgtgcagga
     3061 tttccgcgag atccgattgt cggccctgaa gaaagctcca gagatgttcg ggtccgttta
     3121 cgagcacgag gagaaaaagc ccatggaggc gttcgctgaa cggttgcgag atgccgtggc
     3181 attcggcgcc tacatcgacg gcgagatcat tgggctgtcg gtcttcaaac aggaggacgg
     3241 ccccaaggac gctcacaagg cgcatctgtc cggcgttttc gtggagcccg aacagcgagg
     3301 ccgaggggtc gccggtatgc tgctgcgggc gttgccggcg ggtttattgc tcgtgatgat
     3361 cgtccgacag attccaacgg gaatctggtg gatgcgcatc ttcatcctcg gcgcacttaa
     3421 tatttcgcta ttctggagct tgttgtttat ttcggtctac cgcctgccgg gcggggtcgc
     3481 ggcgacggta ggcgctgtgc agccgctgat ggtcgtgttc atctctgccg ctctgctagg
     3541 tagcccgata cgattgatgg cggtcctggg ggctatttgc ggaactgcgg gcgtggcgct
     3601 gttggtgttg acaccaaacg cagcgctaga tcctgtcggc gtcgcagcgg gcctggcggg
     3661 ggcggtttcc atggcgttcg gaaccgtgct gacccgcaag tggcaacctc ccgtgcctct
     3721 gctcaccttt accgcctggc aactggcggc cggaggactt ctgctcgttc cagtagcttt
     3781 agtgtttgat ccgccaatcc cgatgcctac aggaaccaat gttctcggcc tggcgtggct
     3841 cggcctgatc ggagcgggtt taacctactt cctttggttc cgggggatct cgcgactcga
     3901 acctacagtt gtttccttac tgggctttct cagccgggat ggcgctaaga agctattgcc
     3961 gccgatcttc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     4021 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4381 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     4441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4621 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4741 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atatcaagaa
     4801 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4861 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4921 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4981 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5041 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5101 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5161 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaaacaa
     5221 gtggcagcaa cggattcgca aacctgtcac gccttttgtg ccaaaagccg cgccaggttt
     5281 gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt
     5341 ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct
     5401 atccgaccaa ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat
     5461 ctcggatgat gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct
     5521 tgtcgggaag attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt
     5581 tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa
     5641 gtgggcacta agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc
     5701 tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta
     5761 taaaactaga cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc
     5821 acaggatgac gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca
     5881 ggagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     5941 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     6001 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     6061 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     6121 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     6181 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     6241 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     6301 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     6361 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     6421 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     6481 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     6541 gccggcccag tatcagcccg tcatacttga agctaggcag gcttatcttg gacaagaaga
     6601 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga aaggcgagat
     6661 caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc gcttcgcggc
     6721 gcggcttaac tcaagcgtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt
     6781 tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt
     6841 agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca tttctccaag
     6901 caactacgac aactccataa gcaattacga caatagtcca tcaaattacg acaactctga
     6961 gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa atcgtaggct
     7021 tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg ccaacaatgg
     7081 gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa aaggggggcg
     7141 cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca taaatggcca
     7201 attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca actagcctgc
     7261 tctctaataa aatgttagga gcttggctgc catttttggg gtgaggccgt tcgcggccga
     7321 ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg gttcgagaag
     7381 ggggggcacc ccccttcggc gtgcgcggtc acgcgccagg gcgcagccct ggttaaaaac
     7441 aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa
     7501 cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa
     7561 taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca
     7621 tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt
     7681 ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg
     7741 ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg
     7801 tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc
     7861 cgcgaggtat ccacaacgcc ggcggccggc cgcggtgtct cgcacacggc ttcgacggcg
     7921 tttctggcgc gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc
     7981 cggtgagcgt cggaaagggt cgacccgagg ggaaccctgt ggttggcatg cacatacaaa
     8041 tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct
     8101 cttttctctt aggtttacta tggaatatat cctgggtgtg gccgaagtct gttcgggaag
     8161 ctttaattca atgggtttac ccaaagaaag gaaaattctt caaacaaatt tgagctctgc
     8221 atcttttcaa ggtaccatta tatggtcagt gtgctcgaga gagagaaatg aatctagaat
     8281 tttcaagcgg ccgcaacaaa tatcgatgaa ttcggccggg agcatgcgac gtcgggccca
     8341 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     8401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     8461 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8521 gcgaatggaa attgtaaacg ttaatgggtt tctggagttt aatgagctaa gcacatacgt
     8581 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
     8641 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
     8701 atattcactc tcaatccaaa taatctgcaa tggcaattac cttatccgca acttctttac
     8761 ctatttccgc ccggatccgg gcaggttctc cggccgcttg ggtggagagg ctattcggct
     8821 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     8881 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     8941 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     9001 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     9061 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     9121 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     9181 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     9241 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     9301 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     9361 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     9421 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     9481 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     9541 agttcttctg agcgggactc tggggttcga aatgatcaac ctgccatcac gagatttcga
     9601 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9661 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg atccaacact
     9721 tacgtttgca acgtccaaga gcaaatagac cacgaacgcc ggaaggttgc cgcagcgtgt
     9781 ggattgcgtc tcaattctct cttgcaggaa tgcaatgatg aatatgatac tgactatgaa
     9841 actttgaggg aatactgcct agcaccgtca cctcataacg tgcatcatgc atgccctgac
     9901 aacatggaac atcgctattt ttctgaagaa ttatgctcgt tggaggatgt cgcggcaatt
     9961 gcagctattg ccaacatcga actacccctc acgcatgcat tcatcaatat tattcatgcg
    10021 gggaaaggca agattaatcc aactggcaaa tcatccagcg tgattggtaa cttcagttcc
    10081 agcgacttga ttcgttttgg tgctacccac gttttcaata aggacgagat ggtggagtaa
    10141 agaaggagtg cgtcgaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
    10201 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
    10261 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    10321 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    10381 tatcgcgcca tggatcgatt cgcctctgga aaagggagga cgtgctatat tgatttttga
    10441 ttcactctca agcttgccct tatattacat gtcgatatat cctgcccaag cttccagcca
    10501 gccaacagct ccccgaccag atctgtcgac
//
