LOCUS       62056_3165        9411 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        132..511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        512..715
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        760..778
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             829..849
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             847..1533
                     /codon_start=1
                     /label=APOBEC-1
                     /note="cytidine deaminase (C to U editing enzyme) from rat"
                     /translation="VSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1585..5685
                     /codon_start=1
                     /label=Cas9(D10A)
                     /note="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6430..6450
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    6524..6700
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7254..7270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7278..7294)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7302..7332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7347..7368)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7656..8244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8418..9275)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9276..9380)
                     /label=AmpR promoter
ORIGIN
        1 gccaagctaa ttcgagctcg gtacctgacc cggtcgtgcc cctctctaga taaaggacaa
       61 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      121 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      181 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      241 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      301 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      361 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      421 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      481 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      541 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      601 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      661 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      721 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      781 gacccaagct ggctagcgtt taaacttaag cttgtcgacg gatccatgcc aaagaagaag
      841 aggaaggttt catcggagac cggccctgtt gctgttgacc ccaccctgcg gcggagaatc
      901 gagccacacg agttcgaggt gttcttcgac ccaagggagc tccgcaagga gacgtgcctc
      961 ctgtacgaga tcaactgggg cggcaggcac tccatctgga ggcacaccag ccaaaacacc
     1021 aacaagcacg tggaggtcaa cttcatcgag aagttcacca ccgagaggta cttctgccca
     1081 aacacccgct gctccatcac ctggttcctg tcctggagcc catgcggcga gtgctccagg
     1141 gccatcaccg agttcctcag ccgctaccca cacgtcaccc tgttcatcta catcgccagg
     1201 ctctaccacc acgccgaccc aaggaacagg cagggcctcc gcgacctgat ctccagcggc
     1261 gtgaccatcc aaatcatgac cgagcaggag tccggctact gctggaggaa cttcgtcaac
     1321 tactccccaa gcaacgaggc ccactggcca aggtacccac acctctgggt gcgcctctac
     1381 gtgctcgagc tgtactgcat catcctcggc ctgccaccat gcctcaacat cctgaggcgc
     1441 aagcaaccac agctgacctt cttcaccatc gccctccaaa gctgccacta ccagaggctc
     1501 ccaccacaca tcctgtgggc taccggcctc aagtccggca gcgagacgcc aggcacctcc
     1561 gagagcgcta cgcctgaact taaggacaag aagtactcga tcggcctcgc catcgggacg
     1621 aactcagttg gctgggccgt gatcaccgac gagtacaagg tgccctctaa gaagttcaag
     1681 gtcctgggga acaccgaccg ccattccatc aagaagaacc tcatcggcgc tctcctgttc
     1741 gacagcgggg agaccgctga ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg
     1801 agaaggaaga acaggatctg ctacctccaa gagattttct ccaacgagat ggccaaggtt
     1861 gacgattcat tcttccaccg cctggaggag tctttcctcg tggaggagga taagaagcac
     1921 gagcggcatc ccatcttcgg caacatcgtg gacgaggttg cctaccacga gaagtaccct
     1981 acgatctacc atctgcggaa gaagctcgtg gactccaccg ataaggcgga cctcagactg
     2041 atctacctcg ctctggccca catgatcaag ttccgcggcc atttcctgat cgagggggat
     2101 ctcaacccag acaacagcga tgttgacaag ctgttcatcc aactcgtgca gacctacaac
     2161 caactcttcg aggagaaccc gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc
     2221 gcgaggctct cgaagtccag gaggctggag aacctgatcg ctcagctccc aggcgagaag
     2281 aagaacggcc tgttcgggaa cctcatcgct ctcagcctgg ggctcacccc gaacttcaag
     2341 tcgaacttcg atctcgctga ggacgccaag ctgcaactct ccaaggacac ctacgacgat
     2401 gacctcgata acctcctggc ccagatcggc gatcaatacg cggacctgtt cctcgctgcc
     2461 aagaacctgt cggacgccat cctcctgtca gatatcctcc gcgtgaacac cgagatcacg
     2521 aaggctccac tctctgcctc catgatcaag cgctacgacg agcaccatca ggatctgacc
     2581 ctcctgaagg cgctggtccg ccaacagctc ccggagaagt acaaggagat tttcttcgat
     2641 cagtcgaaga acggctacgc tgggtacatc gacggcgggg cctcacaaga ggagttctac
     2701 aagttcatca agccaatcct ggagaagatg gacggcacgg aggagctcct ggtgaagctc
     2761 aacagggagg acctcctgcg gaagcagaga accttcgata acggcagcat cccccaccaa
     2821 atccatctcg gggagctgca cgccatcctg agaaggcaag aggacttcta ccctttcctc
     2881 aaggataacc gggagaagat cgagaagatc ctgaccttca gaatcccata ctacgtcggc
     2941 cctctcgcgc gggggaactc aagattcgct tggatgaccc gcaagtctga ggagaccatc
     3001 acgccgtgga acttcgagga ggtggtggac aagggcgcta gcgctcagtc gttcatcgag
     3061 aggatgacca acttcgacaa gaacctgccc aacgagaagg tgctccctaa gcactcgctc
     3121 ctgtacgagt acttcaccgt ctacaacgag ctcacgaagg tgaagtacgt caccgagggc
     3181 atgcgcaagc cagcgttcct gtccggggag cagaagaagg ctatcgtgga cctcctgttc
     3241 aagaccaacc ggaaggtcac ggttaagcaa ctcaaggagg actacttcaa gaagatcgag
     3301 tgcttcgatt cggtcgagat cagcggcgtt gaggaccgct tcaacgccag cctcgggacc
     3361 taccacgatc tcctgaagat catcaaggat aaggacttcc tggacaacga ggagaacgag
     3421 gatatcctgg aggacatcgt gctgaccctc acgctgttcg aggacaggga gatgatcgag
     3481 gagcgcctga agacgtacgc ccatctcttc gatgacaagg tcatgaagca actcaagcgc
     3541 cggagataca ccggctgggg gaggctgtcc cgcaagctca tcaacggcat ccgggacaag
     3601 cagtccggga agaccatcct cgacttcctc aagagcgatg gcttcgccaa caggaacttc
     3661 atgcaactga tccacgatga cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg
     3721 agcggccagg gggactcgct gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc
     3781 aagaagggca tcctccagac cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg
     3841 cacaagcctg agaacatcgt catcgagatg gccagagaga accaaaccac gcagaagggg
     3901 caaaagaact ctagggagcg catgaagcgc atcgaggagg gcatcaagga gctggggtcc
     3961 caaatcctca aggagcaccc agtggagaac acccaactgc agaacgagaa gctctacctg
     4021 tactacctcc agaacggcag ggatatgtac gtggaccaag agctggatat caaccgcctc
     4081 agcgattacg acgtcgatca tatcgttccc cagtctttcc tgaaggatga ctccatcgac
     4141 aacaaggtcc tcaccaggtc ggacaagaac cgcggcaagt cagataacgt tccatctgag
     4201 gaggtcgtta agaagatgaa gaactactgg aggcagctcc tgaacgccaa gctgatcacg
     4261 caaaggaagt tcgacaacct caccaaggct gagagaggcg ggctctcaga gctggacaag
     4321 gccggcttca tcaagcggca gctggtcgag accagacaaa tcacgaagca cgttgcgcaa
     4381 atcctcgact ctcggatgaa cacgaagtac gatgagaacg acaagctgat cagggaggtt
     4441 aaggtgatca ccctgaagtc taagctcgtc tccgacttca ggaaggattt ccagttctac
     4501 aaggttcgcg agatcaacaa ctaccaccat gcccatgacg cttacctcaa cgctgtggtc
     4561 ggcaccgctc tgatcaagaa gtacccaaag ctggagtccg agttcgtgta cggggactac
     4621 aaggtttacg atgtgcgcaa gatgatcgcc aagtcggagc aagagatcgg caaggctacc
     4681 gccaagtact tcttctactc aaacatcatg aacttcttca agaccgagat cacgctggcc
     4741 aacggcgaga tccggaagag accgctcatc gagaccaacg gcgagacggg ggagatcgtg
     4801 tgggacaagg gcagggattt cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac
     4861 atcgtcaaga agaccgaggt ccaaacgggc gggttctcaa aggagtctat cctgcctaag
     4921 cggaacagcg acaagctcat cgccagaaag aaggactggg acccaaagaa gtacggcggg
     4981 ttcgacagcc ctaccgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag
     5041 tccaagaagc tcaagagcgt gaaggagctc ctggggatca ccatcatgga gaggtccagc
     5101 ttcgagaaga acccaatcga cttcctggag gccaagggct acaaggaggt gaagaaggac
     5161 ctgatcatca agctcccgaa gtactctctc ttcgagctgg agaacggcag gaagagaatg
     5221 ctggcttccg ctggcgagct ccagaagggg aacgagctcg cgctgccaag caagtacgtg
     5281 aacttcctct acctggcttc ccactacgag aagctcaagg gcagcccgga ggacaacgag
     5341 caaaagcagc tgttcgtcga gcagcacaag cattacctcg acgagatcat cgagcaaatc
     5401 tccgagttca gcaagcgcgt gatcctcgcc gacgcgaacc tggataaggt cctctccgcc
     5461 tacaacaagc accgggacaa gcccatcaga gagcaagcgg agaacatcat ccatctcttc
     5521 accctgacga acctcggcgc tcctgctgct ttcaagtact tcgacaccac gatcgatcgg
     5581 aagagataca cctccacgaa ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc
     5641 ggcctgtacg agacgaggat cgacctctca caactcggcg gggataagag acccgcagca
     5701 accaagaagg cagggcaagc aaagaagaag aagacgcgtg actccggcgg cagcatgttt
     5761 ggagagagct ggaagaagca cctcagcggg gagttcggga aaccgtattt tatcaagcta
     5821 atgggatttg ttgcagaaga aagaaagcat tacactgttt atccaccccc acaccaagtc
     5881 ttcacctgga cccagatgtg tgacataaaa gatgtgaagg ttgtcatcct gggacaggat
     5941 ccatatcatg gacctaatca agctcacggg ctctgcttta gtgttcaaag gcctgttccg
     6001 cctccgccca gtttggagaa catttataaa gagttgtcta cagacataga ggattttgtt
     6061 catcctggcc atggagattt atctgggtgg gccaagcaag gtgttctcct tctcaacgct
     6121 gtcctcacgg ttcgtgccca tcaagccaac tctcataagg agcgaggctg ggagcagttc
     6181 actgatgcag ttgtgtcctg gctaaatcag aactcgaatg gccttgtttt cttgctctgg
     6241 ggctcttatg ctcagaagaa gggcagtgcc attgatagga agcggcacca tgtactacag
     6301 acggctcatc cctccccttt gtcagtgtat agagggttct ttggatgtag acacttttca
     6361 aagaccaatg agctgctgca gaagtctggc aagaagccca ttgactggaa ggagctgtcg
     6421 ggggggagcc caaagaagaa gcggaaggtg tagtgtacaa gtaacccggg aattcggtac
     6481 gctgaaatca ccagtctctc tctacaaatc tatctctctc tattttctcc ataaataatg
     6541 tgtgagtagt ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga
     6601 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     6661 ctaattccta aaaccaaaat ccagtactaa aatccagatc tcctaaagtc cctatagatc
     6721 tttgtcgtga atataaacca gacacgagac gactaaacct ggagcccaga cgccgttcga
     6781 agctagaagt accgcttagg caggaggccg ttagggaaaa gatgctaagg cagggttggt
     6841 tacgttgact cccccgtagg tttggtttaa atatgatgaa gtggacggaa ggaaggagga
     6901 agacaaggaa ggataaggtt gcaggccctg tgcaaggtaa gaagatggaa atttgataga
     6961 ggtacgctac tatacttata ctatacgcta agggaatgct tgtatttata ccctataccc
     7021 cctaataacc ccttatcaat ttaagaaata atccgcataa gcccccgctt aaaaattggt
     7081 atcagagcca tgaataggtc tatgaccaaa actcaagagg ataaaacctc accaaaatac
     7141 gaaagagttc ttaactctaa agataaaaga tctttcaaga tcaaaactag ttccctcaca
     7201 ccggtgacgg ggatcgcatg cgatatctcg agatctagct tggcgtaatc atggtcatag
     7261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7381 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7441 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8701 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9001 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgccag t
//
