LOCUS       V009479                 5460 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009479
VERSION     V009479
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Park AS, Rubin EJ, Minch KJ, Sherman DR.
  TITLE     Clp-mediated Regulation of WhiB1 is Required for Cell Division in
            Mycobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Park AS, Rubin EJ, Minch KJ, Sherman DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2017) Department of Immunology and Infectious
            Diseases, Harvard School of Public Health, 665 Huntington Ave,
            Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 5460)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5460)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2017) Department of Immunology and Infectious Diseases,
            Harvard School of Public Health, 665 Huntington Ave, Boston, MA
            02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      41..68
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      93..398
                     /regulatory_class="promoter"
     protein_bind    268..285
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      414..419
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(475..506)
                     /regulatory_class="terminator"
     CDS             complement(540..1160)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(1187..1503)
                     /regulatory_class="promoter"
     terminator      complement(1522..1549)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(1731..2319)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2654..3466)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(3750..4862)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     terminator      complement(5409..5455)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 tggcagtcga tcgtacgcta gttaactacg tcgactaagc agaaggccat cctgacggat
       61 ggcctttttg cgtttaatac tgtttaaact ctagaaatat tggatcgtcg gcaccgtcac
      121 ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg
      181 cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg
      241 agaagggtct ctgacgagcg ggagaactcc ctatcagtga tagagtttgt cctccctatc
      301 agtgatagat aggctctggg agtacccgtg tgtacgacca gcacggcata catcatttcg
      361 acgccgagag attcgccgcc cgaaatgagc acgatccgca tgcttaatta agaaggagat
      421 atatcgatgg attggcgcca caaccggagt ctgaagatct ggtaccgcgg ccgcaaaata
      481 aaaaagggga cctctagggt ccccaattaa ttagctaaag cgctagccca tggtcattaa
      541 gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat
      601 aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc
      661 ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct
      721 tccaatacgc aacctaaggt aaaatgcccc acagcgctga gtgcatataa cgcgttctct
      781 agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt
      841 tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat
      901 ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa
      961 aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcag ggcccgctta
     1021 ttttttacat gccaatacaa tgtaggctgc tctacaccaa gcttctgggc gagtttacgg
     1081 gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta
     1141 cttttatcta atctagacat tgcgaagtga ttcctcctga tggtaccatg cggatcgtgc
     1201 tcatttcggg cggcgaatct ctcggcgtcg aaatgatgta tgccgtgctg gtcgtacaca
     1261 cgggtactcc cagagcctac tcgaccgagc acacgcagga caaactcgcc caccccgggt
     1321 gggttctccc gctcgtcaga gacccttctc acaccgggcc tgtgcgtgcg aacgcacaga
     1381 tcaacgccgg gatggcgtag agctcactgc gcagcaccgt ggggatgcgg ccgatcatca
     1441 cgtcgcggat cgtgccgcct cccacggccg tgacggtgcc gacgatccaa tatttctaga
     1501 gtgcatgcag tattaaacgc aaaaaggcca tccgtcagga tggccttctg cttagctaat
     1561 cagcggccgc acgcgttgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1921 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2161 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2341 acggggtctg acgctcagtg gaactagtga ggtctgcctc gtgaagaagg tgttgctgac
     2401 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     2461 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     2521 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt
     2581 caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac aaccaattaa
     2641 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     2701 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     2761 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     2821 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     2881 gagtgacgac tgaatccggt gagaatggca aaagattatg catttctttc cagacttgtt
     2941 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     3001 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     3061 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     3121 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     3181 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     3241 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     3301 gtttcagaaa caactctggc gcatggggct tcccatacaa gcgatagatt gtcgcacctg
     3361 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     3421 ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     3481 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     3541 tgtaacatca gagattttga gacacaacgt cgctttgttg ctagctggcg ggaacggcaa
     3601 tgctcctggc ggcggcgggg ctggtgccca ggtctccgca cagagcggcg gtgctggcgc
     3661 tcgcggccag gcgtggttct tcgcgtactg acaagaaacc cccctcttta ggactcagtg
     3721 tccttgggag gggggctttt tgcgtttcag gaggtcttgg ccagcttgga catcgcctca
     3781 gcgatagcct cgtcgcgggc ctcagacgcc atctggtact tcatcgccat cctaggagtc
     3841 gtgtgaccga gacgggccat cagctccttg gtcgtcgcac ctgcctgagc ggcgaacgta
     3901 gcgccgacag cgcggaggtc gtggatgcgg agttccggcc gaccgatctt ggcgtagcca
     3961 cgcttcagcg acttggtgaa cgcggacttc gacagccggt tgccctgcgt cgtggtcacc
     4021 aggaatgcct cggggccctt gttcatcttc gtacggtcct tcatgtgcgc tcggatcatc
     4081 tccgcgacgt gaggcggaac cgtcacagga cgcttcgacc ggacggtctt ggcgttgcca
     4141 acgacgatct tgttccccac gcgggaagcg ccacggcgca cccggagctt catcgtcatg
     4201 ccgtcgtcca cgatgtcctt gcggcgaagc tcgatcagct ctccgaaccg gaggctcgtc
     4261 cacgccagga tgtatgccgc gatccggtag tgctcgaaga tctcagcggc gacgatgtcc
     4321 agctcctcag gcgtcagcgc ctctacgtcg cgctcatcgg ctgccttctg ctcgatccgg
     4381 cacgggttct ctgcgatcag cttgtcctcg accgctgtgt tcatcaccgc ccggaggacg
     4441 ttgtaggcat gccggcgggc agtcgggtgc ttcctaccca tcccggccca ccacgcacgc
     4501 accagagctg gcgtcatctc tgtgaccgcc acttcaccta gcaccgggta gatgcggcgc
     4561 tccgcgtgcc cgctgtacag atccctggtg ccgtctgcga ggtcgcgctc cacgagccac
     4621 ttccgggtgt actcctccag cgtgatggcg ctggcggctg ccttcttcgc ccggtcctgt
     4681 ggaggggtcc aggtctccat ctcgatgagc cgcttctcgc ccgcgagcca ggcttcggcg
     4741 tccatcttgt tgtcgtaggt ctgcagcgcg tagtacctca caccgtcctg cgggttgacg
     4801 tatgaggctt ggatcctccc gctgcgctga gtcttcagcg atccccatcc gcgacgtgcc
     4861 aactaggtct cctctcgtcg tgaacaaggc taccgggttg caactcctgt gcaactctca
     4921 ggcttcaacg cgcttctacg acctgcaatt tctttccact tagaggatgc agccgagagg
     4981 gggtaaaaac ctatcttgac cggcccatat atgtggtcgg cagacaccca ttcttccaaa
     5041 ctagctacgc gggttcgatt cccgtcgccc gctccgctgg tcagagggtg ttttcgccct
     5101 ctggccattt ttctttccag gggtctgcaa ctcttgtgcg actcttctga cctgggcata
     5161 cgcggttgca acgcatccct gatctggcta ctttcgatgc tgacaaacga atagagcccc
     5221 ccgcctgcgc gaacagacga ggggcattca caccagattg gagctggtgc agtgaagaga
     5281 atagaccggg acaaggttgc accgggagtt gcagcggtcg gaaccctcgc cgtcggcggg
     5341 ctggcgttcg ccctgtcgtt cacggctcta gctgatcacc gcggccatga tggccggaca
     5401 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
//
