LOCUS       V009480                 5724 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009480
VERSION     V009480
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Clp-mediated Regulation of WhiB1 is Required for Cell Division in
            Mycobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) Department of Immunology and Infectious
            Diseases, Harvard School of Public Health, 665 Huntington Ave,
            Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 5724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) Department of Immunology and Infectious Diseases,
            Harvard School of Public Health, 665 Huntington Ave, Boston, MA
            02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      41..68
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      93..398
                     /regulatory_class="promoter"
     protein_bind    268..285
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      414..419
                     /regulatory_class="ribosome_binding_site"
     CDS             427..678
                     /gene="whiB1"
                     /label="Transcriptional regulator WhiB1"
                     /note="Transcriptional regulator WhiB1 from Mycobacterium
                     tuberculosis (strain CDC 1551 / Oshkosh). Accession#:
                     P9WF42"
     regulatory      complement(737..768)
                     /regulatory_class="terminator"
     CDS             complement(804..1424)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(1449..1765)
                     /regulatory_class="promoter"
     terminator      complement(1786..1813)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(1995..2583)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2918..3730)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(4014..5126)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     terminator      complement(5673..5719)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 tggcagtcga tcgtacgcta gttaactacg tcgactaagc agaaggccat cctgacggat
       61 ggcctttttg cgtttaatac tgtttaaact ctagaaatat tggatcgtcg gcaccgtcac
      121 ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg
      181 cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg
      241 agaagggtct ctgacgagcg ggagaactcc ctatcagtga tagagtttgt cctccctatc
      301 agtgatagat aggctctggg agtacccgtg tgtacgacca gcacggcata catcatttcg
      361 acgccgagag attcgccgcc cgaaatgagc acgatccgca tgcttaatta agaaggagat
      421 atatcgatgg attggcgcca caaggcggtc tgtcgcgacg aggatccgga gctgttcttc
      481 ccggtgggaa acagtgggcc ggcccttgcc cagatcgctg acgcgaagct ggtgtgcaac
      541 cggtgcccgg tgaccaccga atgcctgagc tgggctttgg agtcgggcca ggacgccggc
      601 gtgtggggcg gcatgagcga ggacgagcgc cgtgcactca agcgtcgcaa cgcgcgcacc
      661 aaggcgcgca ccggagtctg agaattctgc agctggatcc atggatatct agatttaaag
      721 atctggtacc gcggccgcaa aataaaaaag gggacctcta gggtccccaa ttaattagct
      781 aaagcgctag cccatggtca ttaagaccca ctttcacatt taagttgttt ttctaatccg
      841 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
      901 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
      961 ttagcgactt gatgctcttg atcttccaat acgcaaccta aggtaaaatg ccccacagcg
     1021 ctgagtgcat ataacgcgtt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga
     1081 ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca
     1141 tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc
     1201 cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct
     1261 aaggcgtcga gcagggcccg cttatttttt acatgccaat acaatgtagg ctgctctaca
     1321 ccaagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc
     1381 tctaatgcgc tgttaatcac tttactttta tctaatctag acattgcgaa gtgattcctc
     1441 ctgatggtac catgcggatc gtgctcattt cgggcggcga atctctcggc gtcgaaatga
     1501 tgtatgccgt gctggtcgta cacacgggta ctcccagagc ctactcgacc gagcacacgc
     1561 aggacaaact cgcccacccc gggtgggttc tcccgctcgt cagagaccct tctcacaccg
     1621 ggcctgtgcg tgcgaacgca cagatcaacg ccgggatggc gtagagctca ctgcgcagca
     1681 ccgtggggat gcggccgatc atcacgtcgc ggatcgtgcc gcctcccacg gccgtgacgg
     1741 tgccgacgat ccaatatttc tagagtgcat gcagtattaa acgcaaaaag gccatccgtc
     1801 aggatggcct tctgcttagc taatcagcgg ccgcacgcgt tgcgctcggt cgttcggctg
     1861 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1921 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2161 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     2221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2401 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacta gtgaggtctg
     2641 cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag
     2701 aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg
     2761 aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc
     2821 aactcagcaa aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc
     2881 tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
     2941 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     3001 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     3061 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     3121 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagat
     3181 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     3241 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     3301 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     3361 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     3421 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     3481 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     3541 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatgg ggcttcccat
     3601 acaagcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     3661 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
     3721 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     3781 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtcgcttt
     3841 gttgctagct ggcgggaacg gcaatgctcc tggcggcggc ggggctggtg cccaggtctc
     3901 cgcacagagc ggcggtgctg gcgctcgcgg ccaggcgtgg ttcttcgcgt actgacaaga
     3961 aacccccctc tttaggactc agtgtccttg ggaggggggc tttttgcgtt tcaggaggtc
     4021 ttggccagct tggacatcgc ctcagcgata gcctcgtcgc gggcctcaga cgccatctgg
     4081 tacttcatcg ccatcctagg agtcgtgtga ccgagacggg ccatcagctc cttggtcgtc
     4141 gcacctgcct gagcggcgaa cgtagcgccg acagcgcgga ggtcgtggat gcggagttcc
     4201 ggccgaccga tcttggcgta gccacgcttc agcgacttgg tgaacgcgga cttcgacagc
     4261 cggttgccct gcgtcgtggt caccaggaat gcctcggggc ccttgttcat cttcgtacgg
     4321 tccttcatgt gcgctcggat catctccgcg acgtgaggcg gaaccgtcac aggacgcttc
     4381 gaccggacgg tcttggcgtt gccaacgacg atcttgttcc ccacgcggga agcgccacgg
     4441 cgcacccgga gcttcatcgt catgccgtcg tccacgatgt ccttgcggcg aagctcgatc
     4501 agctctccga accggaggct cgtccacgcc aggatgtatg ccgcgatccg gtagtgctcg
     4561 aagatctcag cggcgacgat gtccagctcc tcaggcgtca gcgcctctac gtcgcgctca
     4621 tcggctgcct tctgctcgat ccggcacggg ttctctgcga tcagcttgtc ctcgaccgct
     4681 gtgttcatca ccgcccggag gacgttgtag gcatgccggc gggcagtcgg gtgcttccta
     4741 cccatcccgg cccaccacgc acgcaccaga gctggcgtca tctctgtgac cgccacttca
     4801 cctagcaccg ggtagatgcg gcgctccgcg tgcccgctgt acagatccct ggtgccgtct
     4861 gcgaggtcgc gctccacgag ccacttccgg gtgtactcct ccagcgtgat ggcgctggcg
     4921 gctgccttct tcgcccggtc ctgtggaggg gtccaggtct ccatctcgat gagccgcttc
     4981 tcgcccgcga gccaggcttc ggcgtccatc ttgttgtcgt aggtctgcag cgcgtagtac
     5041 ctcacaccgt cctgcgggtt gacgtatgag gcttggatcc tcccgctgcg ctgagtcttc
     5101 agcgatcccc atccgcgacg tgccaactag gtctcctctc gtcgtgaaca aggctaccgg
     5161 gttgcaactc ctgtgcaact ctcaggcttc aacgcgcttc tacgacctgc aatttctttc
     5221 cacttagagg atgcagccga gagggggtaa aaacctatct tgaccggccc atatatgtgg
     5281 tcggcagaca cccattcttc caaactagct acgcgggttc gattcccgtc gcccgctccg
     5341 ctggtcagag ggtgttttcg ccctctggcc atttttcttt ccaggggtct gcaactcttg
     5401 tgcgactctt ctgacctggg catacgcggt tgcaacgcat ccctgatctg gctactttcg
     5461 atgctgacaa acgaatagag ccccccgcct gcgcgaacag acgaggggca ttcacaccag
     5521 attggagctg gtgcagtgaa gagaatagac cgggacaagg ttgcaccggg agttgcagcg
     5581 gtcggaaccc tcgccgtcgg cgggctggcg ttcgccctgt cgttcacggc tctagctgat
     5641 caccgcggcc atgatggccg gacaaacaac agataaaacg aaaggcccag tctttcgact
     5701 gagcctttcg ttttatttga tgcc
//
