LOCUS       V009481                 5722 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009481
VERSION     V009481
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Clp-mediated Regulation of WhiB1 is Required for Cell Division in
            Mycobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) Department of Immunology and Infectious
            Diseases, Harvard School of Public Health, 665 Huntington Ave,
            Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 5722)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5722)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) Department of Immunology and Infectious Diseases,
            Harvard School of Public Health, 665 Huntington Ave, Boston, MA
            02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      41..68
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      93..398
                     /regulatory_class="promoter"
     protein_bind    268..285
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      414..419
                     /regulatory_class="ribosome_binding_site"
     CDS             427..678
                     /gene="whiB1"
                     /label="Transcriptional regulator WhiB1"
                     /note="Transcriptional regulator WhiB1 from Mycobacterium
                     tuberculosis (strain CDC 1551 / Oshkosh). Accession#:
                     P9WF42"
     regulatory      complement(737..768)
                     /regulatory_class="terminator"
     CDS             complement(802..1422)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(1449..1765)
                     /regulatory_class="promoter"
     terminator      complement(1784..1811)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2916..3728)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(4012..5124)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     terminator      complement(5671..5717)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 tggcagtcga tcgtacgcta gttaactacg tcgactaagc agaaggccat cctgacggat
       61 ggcctttttg cgtttaatac tgtttaaact ctagaaatat tggatcgtcg gcaccgtcac
      121 ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg
      181 cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg
      241 agaagggtct ctgacgagcg ggagaactcc ctatcagtga tagagtttgt cctccctatc
      301 agtgatagat aggctctggg agtacccgtg tgtacgacca gcacggcata catcatttcg
      361 acgccgagag attcgccgcc cgaaatgagc acgatccgca tgcttaatta agaaggagat
      421 atatcgatgg attggcgcca caaggcggtc tgtcgcgacg aggatccgga gctgttcttc
      481 ccggtgggaa acagtgggcc ggcccttgcc cagatcgctg acgcgaagct ggtgtgcaac
      541 cggtgcccgg tgaccaccga atgcctgagc tgggctttgg agtcgggcca ggacgccggc
      601 gtgtggggcg gcatgagcga ggacgagcgc cgtgcactca agcgtgagaa cgcgcgcacc
      661 aaggcgcgca ccggagtctg aattctgcag ctggatccat ggatatctag atttaaagat
      721 ctggtaccgc ggccgcaaaa taaaaaaggg gacctctagg gtccccaatt aattagctaa
      781 agcgctagcc catggtcatt aagacccact ttcacattta agttgttttt ctaatccgca
      841 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      901 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      961 agcgacttga tgctcttgat cttccaatac gcaacctaag gtaaaatgcc ccacagcgct
     1021 gagtgcatat aacgcgttct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
     1081 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
     1141 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
     1201 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
     1261 ggcgtcgagc agggcccgct tattttttac atgccaatac aatgtaggct gctctacacc
     1321 aagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
     1381 taatgcgctg ttaatcactt tacttttatc taatctagac attgcgaagt gattcctcct
     1441 gatggtacca tgcggatcgt gctcatttcg ggcggcgaat ctctcggcgt cgaaatgatg
     1501 tatgccgtgc tggtcgtaca cacgggtact cccagagcct actcgaccga gcacacgcag
     1561 gacaaactcg cccaccccgg gtgggttctc ccgctcgtca gagacccttc tcacaccggg
     1621 cctgtgcgtg cgaacgcaca gatcaacgcc gggatggcgt agagctcact gcgcagcacc
     1681 gtggggatgc ggccgatcat cacgtcgcgg atcgtgccgc ctcccacggc cgtgacggtg
     1741 ccgacgatcc aatatttcta gagtgcatgc agtattaaac gcaaaaaggc catccgtcag
     1801 gatggccttc tgcttagcta atcagcggcc gcacgcgttg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaactagt gaggtctgcc
     2641 tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat ccagccagaa
     2701 agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa
     2761 cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa
     2821 ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc
     2881 tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     2941 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     3001 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     3061 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     3121 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagatta
     3181 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     3241 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     3301 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     3361 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     3421 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     3481 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     3541 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatgggg cttcccatac
     3601 aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3661 aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata
     3721 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     3781 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtcgctttgt
     3841 tgctagctgg cgggaacggc aatgctcctg gcggcggcgg ggctggtgcc caggtctccg
     3901 cacagagcgg cggtgctggc gctcgcggcc aggcgtggtt cttcgcgtac tgacaagaaa
     3961 cccccctctt taggactcag tgtccttggg aggggggctt tttgcgtttc aggaggtctt
     4021 ggccagcttg gacatcgcct cagcgatagc ctcgtcgcgg gcctcagacg ccatctggta
     4081 cttcatcgcc atcctaggag tcgtgtgacc gagacgggcc atcagctcct tggtcgtcgc
     4141 acctgcctga gcggcgaacg tagcgccgac agcgcggagg tcgtggatgc ggagttccgg
     4201 ccgaccgatc ttggcgtagc cacgcttcag cgacttggtg aacgcggact tcgacagccg
     4261 gttgccctgc gtcgtggtca ccaggaatgc ctcggggccc ttgttcatct tcgtacggtc
     4321 cttcatgtgc gctcggatca tctccgcgac gtgaggcgga accgtcacag gacgcttcga
     4381 ccggacggtc ttggcgttgc caacgacgat cttgttcccc acgcgggaag cgccacggcg
     4441 cacccggagc ttcatcgtca tgccgtcgtc cacgatgtcc ttgcggcgaa gctcgatcag
     4501 ctctccgaac cggaggctcg tccacgccag gatgtatgcc gcgatccggt agtgctcgaa
     4561 gatctcagcg gcgacgatgt ccagctcctc aggcgtcagc gcctctacgt cgcgctcatc
     4621 ggctgccttc tgctcgatcc ggcacgggtt ctctgcgatc agcttgtcct cgaccgctgt
     4681 gttcatcacc gcccggagga cgttgtaggc atgccggcgg gcagtcgggt gcttcctacc
     4741 catcccggcc caccacgcac gcaccagagc tggcgtcatc tctgtgaccg ccacttcacc
     4801 tagcaccggg tagatgcggc gctccgcgtg cccgctgtac agatccctgg tgccgtctgc
     4861 gaggtcgcgc tccacgagcc acttccgggt gtactcctcc agcgtgatgg cgctggcggc
     4921 tgccttcttc gcccggtcct gtggaggggt ccaggtctcc atctcgatga gccgcttctc
     4981 gcccgcgagc caggcttcgg cgtccatctt gttgtcgtag gtctgcagcg cgtagtacct
     5041 cacaccgtcc tgcgggttga cgtatgaggc ttggatcctc ccgctgcgct gagtcttcag
     5101 cgatccccat ccgcgacgtg ccaactaggt ctcctctcgt cgtgaacaag gctaccgggt
     5161 tgcaactcct gtgcaactct caggcttcaa cgcgcttcta cgacctgcaa tttctttcca
     5221 cttagaggat gcagccgaga gggggtaaaa acctatcttg accggcccat atatgtggtc
     5281 ggcagacacc cattcttcca aactagctac gcgggttcga ttcccgtcgc ccgctccgct
     5341 ggtcagaggg tgttttcgcc ctctggccat ttttctttcc aggggtctgc aactcttgtg
     5401 cgactcttct gacctgggca tacgcggttg caacgcatcc ctgatctggc tactttcgat
     5461 gctgacaaac gaatagagcc ccccgcctgc gcgaacagac gaggggcatt cacaccagat
     5521 tggagctggt gcagtgaaga gaatagaccg ggacaaggtt gcaccgggag ttgcagcggt
     5581 cggaaccctc gccgtcggcg ggctggcgtt cgccctgtcg ttcacggctc tagctgatca
     5641 ccgcggccat gatggccgga caaacaacag ataaaacgaa aggcccagtc tttcgactga
     5701 gcctttcgtt ttatttgatg cc
//
