LOCUS       40924_4584        6010 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector pAP20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Law RJ, Hamlin JN, Sivro A, McCorrister SJ, Cardama GA, Cardona ST.
  TITLE     A functional phenylacetic acid catabolic pathway is required for 
            full pathogenicity of Burkholderia cenocepacia in the Caenorhabditis
            elegans host model
  JOURNAL   J. Bacteriol. 190 (21), 7209-7218 (2008)
  PUBMED    18776009
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Law RJ, Hamlin JNR., Sivro A, McCorrister SJ, Cardama G, Cardona ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2008) Microbiology, University of Manitoba, 45 
            Chancellors Circle, 418 Buller Bldg, Winnipeg, Manitoba R3T 2N2, 
            Canada
REFERENCE   3  (bases 1 to 6010)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6010)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2008"; volume: "190"; issue: "21"; pages: 
            "7209-7218"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-APR-2008) Microbiology, University of Manitoba, 45 Chancellors 
            Circle, 418 Buller Bldg, Winnipeg, Manitoba R3T 2N2, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..185
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      784..870
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      962..989
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        complement(1717..1807)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      3200..3969
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3970..4629
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             complement(5115..5753)
                     /codon_start=1
                     /gene="CAT"
                     /product="chloramphenicol acetyltransferase"
                     /label=CAT
                     /note="chloramphenicol resistance"
                     /protein_id="ACF35273.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR"
     gene            complement(5115..5753)
                     /gene="CAT"
                     /label=CAT
     promoter        complement(5754..5856)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atcttaggat ctcccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
       61 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta
      121 ctcgagtatg ctagcgatga gctcgcacga acccagttga cataagcctg ttcggttcgt
      181 aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
      241 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag
      301 tctagcctcg ggcatccaag ctagctaagc gcgttacgcc gtgggtcgat gtttgatgtt
      361 atggaacagc aacgatgtta cgcagcaggg tagtcgccct aaaacaaagt taggcagccg
      421 ttgtgctggt gctttctagt agttgttgtg gggtaggcag tcagagctcg atttgcttgt
      481 cgccataata gattcacaag aaggattcgg aattctacat atgtcatcga caccatggta
      541 cccggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag
      601 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
      661 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
      721 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
      781 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      841 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      901 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
      961 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc actcactaca
     1021 gcagagccat ttaaacaaca tcccctcccc ctttccaccg cgtcagacgc ccgtagcagc
     1081 ccgctacggg ctttttcatg ccctgcccta gcgtccaagc ctcacggccg cgctcggcct
     1141 ctctggcggc cttctggcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc
     1201 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt
     1261 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
     1321 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa
     1381 gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     1441 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     1501 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     1561 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     1621 acctattaat ttcccctcgt caaaaataag gttatcaagc gagaaatcac catgagtgac
     1681 gactgaatcc ggtgagaatg gcaaaagcta atgcatagct cctgaaaatc tcgataactc
     1741 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1801 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1861 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cgaagacgaa
     1921 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1981 cgtcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc gctgggcctg
     2041 tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg ccttgatgat
     2101 cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggcgtcca gaacgggctt
     2161 caggcgctcc cgaaggtctc gggccgtctc ttgggcttga tcggccttct tgcgcatctc
     2221 acgcgctcct gcggcggcct gtagggcagg ctcatacccc tgccgaaccg cttttgtcag
     2281 ccggtcggcc acggcttccg gcgtctcaac gcgctttgag attcccagct tttcggccaa
     2341 tccctgcggt gcataggcgc gtggctcgac cgcttgcggg ctgatggtga cgtggcccac
     2401 tggtggccgc tccagggcct cgtagaacgc ctgaatgcgc gtgtgacgtg ccttgctgcc
     2461 ctcgatgccc cgttgcagcc ctagatcggc cacagcggcc gcaaacgtgg tctggtcgcg
     2521 ggtcatctgc gctttgttgc cgatgaactc cttggccgac agcctgccgt cctgcgtcag
     2581 cggcaccacg aacgcggtca tgtgcgggct ggtttcgtca cggtggatgc tggccgtcac
     2641 gatgcgatcc gccccgtact tgtccgccag ccacttgtgc gccttctcga agaacgccgc
     2701 ctgctgttct tggctggccg acttccacca ttccgggctg gccgtcatga cgtactcgac
     2761 cgccaacaca gcgtccttgc gccgcttctc tggcagcaac tcgcgcagtc ggcccatcgc
     2821 ttcatcggtg ctgctggccg cccagtgctc gttctctggc gtcctgctgg cgtcagcgtt
     2881 gggcgtctcg cgctcgcggt aggcgtgctt gagactggcc gccacgttgc ccattttcgc
     2941 cagcttcttg catcgcatga tcgcgtatgc cgccatgcct gcccctccct tttggtgtcc
     3001 aaccggctcg acgggggcag cgcaaggcgg tgcctccggc gggccactca atgcttgagt
     3061 atactcacta gactttgctt cgcaaagtcg tgaccgccta cggcggctgc ggcgccctac
     3121 gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca gcccgtggat
     3181 atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc cgtggacaac
     3241 cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca cagggattgc
     3301 ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg gcctgcggcc
     3361 ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc ggcctgcgcg
     3421 cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca gcgaccgcgc
     3481 agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg ggcagtcgaa
     3541 ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc gagtgcgggg gttccaaggg
     3601 ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg atcaacaagc cccggagggg
     3661 ccactttttg ccggaggggg agccgcgccg aaggcgtggg ggaaccccgc aggggtgccc
     3721 ttctttgggc accaaagaac tagatatagg gcgaaatgcg aaagacttaa aaatcaacaa
     3781 cttaaaaaag gggggtacgc aacagctcat tgcggcaccc cccgcaatag ctcattgcgt
     3841 aggttaaaga aaatctgtaa ttgactgcca cttttacgca acgcataatt gttgtcgcgc
     3901 tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc gtgcggcacc ctaccgcatg
     3961 gagataagca tggccacgca gtccagagaa atcggcattc aagccaagaa caagcccggt
     4021 cactgggtgc aaacggaacg caaagcgcat gaggcgtggg ccgggcttat tgcgaggaaa
     4081 cccacggcgg caatgctgct gcatcacctc gtggcgcaga tgggccacca gaacgccgtg
     4141 gtggtcagcc agaagacact ttccaagctc atcggacgtt ctttgcggac ggtccaatac
     4201 gcagtcaagg acttggtggc cgagcgctgg atctccgtcg tgaagctcaa cggccccggc
     4261 accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg gccagccccg cgaccagttg
     4321 cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg acgaccagga cgaatcgctg
     4381 ttggggcatg gcgacctgcg ccgcatcccg accctgtatc cgggcgagca gcaactaccg
     4441 accggccccg gcgaggagcc gcccagccag cccggcattc cgggcatgga accagacctg
     4501 ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc agcagcgcct gccgatgccc
     4561 gatgagccgt gttttctgga cgatggcgag ccgttggagc cgccgacacg ggtcacgctg
     4621 ccgcgccggt agcacttggg ttgcgcagca acccgtaagt gcgctgttcc agactatcgg
     4681 ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     4741 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccgtt
     4801 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg
     4861 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta
     4921 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     4981 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
     5041 ttcaggcgta gcaccaggcg tttaagtcga ccaataactc tacggggtct gacgctcagt
     5101 ggaacgaaat cgattcatcg cagtactgtt gtattcatta agcatctgcc gacatggaag
     5161 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     5221 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     5281 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     5341 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     5401 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     5461 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     5521 ttcattgcca tacgtaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     5581 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     5641 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     5701 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     5761 tccttagctc ctgaaaatct cgacaactca aaaaatacgc ccggtagtga tcttatttca
     5821 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg
     5881 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct
     5941 tccgtcacag gtaggcgcgc cgaagttcct atacttatcg atctgcactt gaacgtgtgg
     6001 cctaagcgat
//
