LOCUS       V009488                 8772 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009488
VERSION     V009488
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Clp-mediated Regulation of WhiB1 is Required for Cell Division in
            Mycobacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   Park AS, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2017) Department of Immunology and Infectious
            Diseases, Harvard School of Public Health, 665 Huntington Ave,
            Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 8772)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2017) Department of Immunology and Infectious Diseases,
            Harvard School of Public Health, 665 Huntington Ave, Boston, MA
            02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(37..83)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     359..375
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    395..415
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             complement(451..1074)
                     /codon_start=1
                     /product="TetR"
                     /label="TetR"
                     /note="Tet repressor protein"
                     /protein_id="AWN08190.1"
                     /translation="MSRLDKSKVINSALALGNEVGIEGVTTRKLAQKLGVAQKTLYWHV
                     KNKRALLDALAVEILARHHDYSLPAAGESWQSFLRNNAMSFRRALLRYRDGAKVHLGTR
                     PDEKQYDTVETQLRFMTENGFSLENALYALSAVGHFTLGCVLEDQEHTAAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     regulatory      complement(1107..1600)
                     /regulatory_class="promoter"
     protein_bind    1600..1624
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1799..1823)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1838..4381
                     /gene="clpC1"
                     /label="ATP-dependent Clp protease ATP-binding subunit
                     ClpC1"
                     /note="ATP-dependent Clp protease ATP-binding subunit ClpC1
                     from Mycobacterium tuberculosis (strain ATCC 25618 /
                     H37Rv). Accession#: P9WPC9"
     polyA_signal    4801..4935
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5131..5719)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6054..6866)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(7150..8262)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
ORIGIN
        1 tgatcaccgc ggccatgatg gccggacaaa caacagataa aacgaaaggc ccagtctttc
       61 gactgagcct ttcgttttat ttgatgcctg gcagtcgatc gtacgctagt taactacgtc
      121 gacatcgatt taattaatat gcatcaattg atttaaatac tagtgttaac tatttaattg
      181 gggaccctag aggtcccctt ttttatttta aaaatttttt cnaaggggac ctctagggtc
      241 cccagctggc tagcgagtca tgaggtnacc atgttctttc ctgcgttatc ccctgattct
      301 gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca atttcacaca
      361 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgcagct
      421 ggctagcgag tcatgaggtn accatggtca ttaggagccg ctctcgcact tcagctgctt
      481 ctccaagccg cagatgatca gctccaggcc gaacaagaag gccggctcgg cgccctggtg
      541 gtcgaacagc tcgatggcct ggcgcagcaa cggcggcatg ctgtcggtgg tcggggtctc
      601 gcgctcctcc ttggcggccg tgtgctcctg gtcctccaac acgcagccca gggtgaagtg
      661 gcccacggcc gacagggcgt acaacgcgtt ctccagcgag aagccgttct cggtcatgaa
      721 gcgcagctgg gtctccacgg tgtcgtactg cttctcgtcc gggcgggtgc ccaggtgcac
      781 cttggcgccg tcgcggtagc gcagcagcgc gcggcggaac gacatggcgt tgttgcgcag
      841 gaacgactgc cacgactcgc cggccgccgg cagcgagtag tcgtggtggc gcgccaggat
      901 ctccaccgcc agggcgtcca gcagggcccg cttgttcttg acgtgccagt acagggtctt
      961 ctgcgcgacg cccagcttct gggccagctt gcgggtggtc acgccctcga tgccgacctc
     1021 gttgcccagg gccagggcgg agttgatgac cttggacttg tccaggcggg acattgcgaa
     1081 gtgattcctc ctgatggtac cctaggtcag atcaacggcc tggtcgacaa gggcaatacg
     1141 gtgatcgtca tcgaacataa cctggacgtg atcggccgtc aactcgttga ggtaccggtc
     1201 ggattcaaat ggttcgtcga cggtctgatc ccgcggtgtg caccgatgcc ctcacggttc
     1261 cagcggtcga ccaggtcctc gccgtgacgc cccttgatcg caaacgccac cgaatgcacc
     1321 acggccacag ccaaagccga cacgctggtt accacgacga tgttcacggg gggccctcgc
     1381 ggatctggcc caactgctgg acttccaccg aagcaatcga gccgatgtca cgctgcaact
     1441 ggtgcgggtg ggcgacccgc gggcattcgg ctgcgtaccc accgacgagg aggaccgcgt
     1501 agtcgccttt ctggagaaga cggagcagac ggtgctgttg ggcgcctgct ggattcccag
     1561 gtcttcgaac accgacacgc tcgcggcgtg atccaatatt caagtttgta caaaaaagca
     1621 ggctgctacc aggcctagat ctggggaccc tagaggtccc cttttttatt ttaaaaattt
     1681 tttcacgtgc cagccgattg gtcgctctga ccagggaaaa tagccctctg acctggggat
     1741 ttgcgatccc cgtcagtgac ggagataatt gtgagcgctc acaaccgcat gcaccaccac
     1801 ccagctttct tgtacaaagt gggaaggaga tatacctatg ttcgaacgat ttaccgaccg
     1861 tgcccgcagg gtcgtcgtcc tggctcagga agaggccagg atgctcaacc acaactacat
     1921 cggcaccgag cacattcttt taggcctgat ccatgaaggg gaaggcgttg ccgccaagtc
     1981 actggagtcg ttggggatct cgctggaagg tgtgcgcagt caggtcgagg agatcatcgg
     2041 ccagggccag caggcgccgt ctgggcacat tccgtttacc ccccgcgcca aaaaggtcct
     2101 cgagctgagc ttgcgtgaag cgctgcagct tggccacaac tacatcggga ccgaacacat
     2161 tttgctgggc ctcatccgag agggtgaagg cgtggccgcc caggtgctgg tcaagctggg
     2221 cgccgagctg acccgggtgc gccagcaggt gatccagctg ctctccggtt accaaggcaa
     2281 ggaggccgcc gaagccggca ccggcggccg cgggggagag tccggctctc cgtctacgtc
     2341 cttggtgctc gaccagttcg gccgcaacct cacggcggcg gcgatggaag gcaaactgga
     2401 cccggtcatc ggccgcgaga aggaaatcga gcgggtcatg caggtgctct ctcggcgcac
     2461 caagaacaac ccggtgctga tcggcgagcc cggcgtcggc aagaccgcgg tcgtcgaagg
     2521 actggcgcag gccatcgtgc acggcgaggt gcccgagacg ctcaaggaca agcagctcta
     2581 cacgctggat ctgggatcgc tggtggcggg tagccgctac cgcggtgact tcgaggaacg
     2641 cctcaagaag gtgctcaagg agatcaacac ccgcggtgac atcatcctgt ttatcgacga
     2701 gctgcacacc ttggtcggtg ctggagccgc cgagggcgcg atcgacgccg cctcgatcct
     2761 gaaaccgaag ctcgctcgcg gtgaactgca aacgatcggc gccaccacgc tcgacgaata
     2821 ccgcaagtac atcgagaagg acgccgcgct ggagcgccgc ttccagccgg tgcaggtggg
     2881 tgagccgacg gtggagcaca ccatcgagat cctcaagggc ctgcgggacc ggtacgaggc
     2941 gcaccaccgg gtgtcgatca ccgatgcggc gatggtggcc gccgcgaccc tggccgaccg
     3001 ctacatcaac gaccggttcc tgcccgacaa ggcgatcgac ctgatcgacg aggcgggtgc
     3061 tcggatgcgg attcgtcgca tgaccgcacc gccagaccta cgcgagttcg atgagaagat
     3121 cgccgaggct cgtcgggaga aggaatcggc tatcgacgcc caggacttcg agaaggccgc
     3181 cagcctgcgc gaccgggaga agacactggt cgcacagcgt gctgagcgcg aaaagcagtg
     3241 gcgttcaggc gatcttgacg tggtcgcgga ggtcgacgac gagcagatcg ccgaggtgct
     3301 gggcaactgg accggtatcc cggtgttcaa gctcaccgag gccgagacca cccggctgtt
     3361 gcggatggaa gaagagctgc acaagcggat catcgggcaa gaggacgccg tcaaggccgt
     3421 ttccaaggcc atccggcgta cccgggccgg gctgaaagac cccaagcgcc cgtcgggctc
     3481 gttcatcttc gccggcccgt ccggtgtcgg taagaccgaa ctgtccaagg cgctggccaa
     3541 cttcttgttc ggtgacgacg acgcgcttat tcagattgac atgggtgaat tccacgaccg
     3601 gttcaccgcg tcgcggctat tcggcgcgcc gcccggatac gtcggctacg aggagggcgg
     3661 ccaactcacc gagaaggtgc ggcgcaagcc gttctcggtg gtgctgttcg acgagatcga
     3721 gaaggcgcat caggagatct acaacagcct gctgcaggtg ctcgaggatg gccggctcac
     3781 cgacgggcag ggccgcacgg tggacttcaa gaacaccgtg ctgatcttta cgtccaatct
     3841 gggcacctcc gacatctcta agccggtcgg tctgggcttt tccaagggcg gcggtgagaa
     3901 cgactacgag cggatgaaac agaaggtcaa cgacgagctg aagaaacact tccgcccgga
     3961 gttcctcaac cgcatcgacg acatcatcgt cttccaccag ctgacccgcg aggagatcat
     4021 ccggatggtc gacctgatga tcagccgggt cgccggccag ctcaagagca aggacatggc
     4081 gctggtgctg accgatgcgg ccaaggcgct gctggccaag cgtggcttcg acccggtgtt
     4141 gggggcccgc ccgttgcggc gcaccatcca gcgtgagatc gaagatcagc tctcggagaa
     4201 gatcctcttc gaggaggtcg ggccgggtca ggtggtcacc gtcgacgtgg acaactggga
     4261 cggtgaaggt cccggcgagg acgcggtgtt caccttcacc ggtacccgca agccgccggc
     4321 cgagccggat ctggccaagg ctggagcgca cagcgcgggc ggcccggagc cggccgcgcg
     4381 ggccgccaac gacgagaact actccgagaa ctacgcggac gccagctagt gacatatggg
     4441 gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
     4501 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
     4561 gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa acttttggat
     4621 gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc cgtttttccc
     4681 gatttggcta catgacatca accatatcag caaaagtgat acgggtatta tttttgccgc
     4741 tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg acaaactcgg
     4801 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4861 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4921 tatcatgtct ggatcctcta gagtcgacct gcaggtttaa acgcggccgc acgcgttgcg
     4981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     5341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     5581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5761 gaactagtga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc
     5821 cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga
     5881 ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg
     5941 cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt
     6001 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
     6061 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     6121 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     6181 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     6241 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     6301 gagaatggca aaagattatg catttctttc cagacttgtt caacaggcca gccattacgc
     6361 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     6421 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     6481 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     6541 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta
     6601 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     6661 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     6721 gcatggggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga
     6781 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa
     6841 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     6901 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     6961 gacacaacgt cgctttgttg ctagctggcg ggaacggcaa tgctcctggc ggcggcgggg
     7021 ctggtgccca ggtctccgca cagagcggcg gtgctggcgc tcgcggccag gcgtggttct
     7081 tcgcgtactg acaagaaacc cccctcttta ggactcagtg tccttgggag gggggctttt
     7141 tgcgtttcag gaggtcttgg ccagcttgga catcgcctca gcgatagcct cgtcgcgggc
     7201 ctcagacgcc atctggtact tcatcgccat cctaggagtc gtgtgaccga gacgggccat
     7261 cagctccttg gtcgtcgcac ctgcctgagc ggcgaacgta gcgccgacag cgcggaggtc
     7321 gtggatgcgg agttccggcc gaccgatctt ggcgtagcca cgcttcagcg acttggtgaa
     7381 cgcggacttc gacagccggt tgccctgcgt cgtggtcacc aggaatgcct cggggccctt
     7441 gttcatcttc gtacggtcct tcatgtgcgc tcggatcatc tccgcgacgt gaggcggaac
     7501 cgtcacagga cgcttcgacc ggacggtctt ggcgttgcca acgacgatct tgttccccac
     7561 gcgggaagcg ccacggcgca cccggagctt catcgtcatg ccgtcgtcca cgatgtcctt
     7621 gcggcgaagc tcgatcagct ctccgaaccg gaggctcgtc cacgccagga tgtatgccgc
     7681 gatccggtag tgctcgaaga tctcagcggc gacgatgtcc agctcctcag gcgtcagcgc
     7741 ctctacgtcg cgctcatcgg ctgccttctg ctcgatccgg cacgggttct ctgcgatcag
     7801 cttgtcctcg accgctgtgt tcatcaccgc ccggaggacg ttgtaggcat gccggcgggc
     7861 agtcgggtgc ttcctaccca tcccggccca ccacgcacgc accagagctg gcgtcatctc
     7921 tgtgaccgcc acttcaccta gcaccgggta gatgcggcgc tccgcgtgcc cgctgtacag
     7981 atccctggtg ccgtctgcga ggtcgcgctc cacgagccac ttccgggtgt actcctccag
     8041 cgtgatggcg ctggcggctg ccttcttcgc ccggtcctgt ggaggggtcc aggtctccat
     8101 ctcgatgagc cgcttctcgc ccgcgagcca ggcttcggcg tccatcttgt tgtcgtaggt
     8161 ctgcagcgcg tagtacctca caccgtcctg cgggttgacg tatgaggctt ggatcctccc
     8221 gctgcgctga gtcttcagcg atccccatcc gcgacgtgcc aactaggtct cctctcgtcg
     8281 tgaacaaggc taccgggttg caactcctgt gcaactctca ggcttcaacg cgcttctacg
     8341 acctgcaatt tctttccact tagaggatgc agccgagagg gggtaaaaac ctatcttgac
     8401 cggcccatat atgtggtcgg cagacaccca ttcttccaaa ctagctacgc gggttcgatt
     8461 cccgtcgccc gctccgctgg tcagagggtg ttttcgccct ctggccattt ttctttccag
     8521 gggtctgcaa ctcttgtgcg actcttctga cctgggcata cgcggttgca acgcatccct
     8581 gatctggcta ctttcgatgc tgacaaacga atagagcccc ccgcctgcgc gaacagacga
     8641 ggggcattca caccagattg gagctggtgc agtgaagaga atagaccggg acaaggttgc
     8701 accgggagtt gcagcggtcg gaaccctcgc cgtcggcggg ctggcgttcg ccctgtcgtt
     8761 cacggctcta gc
//
