LOCUS       40924_4574        5675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pAP1-Luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Zheng C-F.
  TITLE     pAP1-Luc reporter plasmid for detection of the activator protein 1 
            transduction pathway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 5675)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   4  (bases 1 to 5675)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   5  (bases 1 to 5675)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAR-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (29-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (26-JAN-1999) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Jan 26, 1999 this sequence version replaced gi:3808230.
FEATURES             Location/Qualifiers
     source          1..5675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /label=AmpR promoter
     CDS             137..994
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1168..1755
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1934..2074)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2539..2671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2737..2768
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2795..4444
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4578..4643
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5213..5347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
     2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
     2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
     2641 actcatcaat gtatcttatc atgtctggat ccaagcttga ctaatgacta atgactaatg
     2701 actaatgact aatgactaat gactaagcgg agactctaga gggtatataa tggaagctcg
     2761 aattccagct tggcattccg gtactgttgg taaaatggaa gacgccaaaa acataaagaa
     2821 aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc
     2881 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
     2941 gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
     3001 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
     3061 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     3121 taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc
     3181 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat
     3241 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
     3301 atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga
     3361 caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc
     3421 ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa
     3481 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
     3541 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
     3601 agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc
     3661 aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
     3721 acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa
     3781 acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat
     3841 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
     3901 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga
     3961 attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
     4021 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
     4081 cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt
     4141 ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt
     4201 ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
     4261 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
     4321 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
     4381 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa
     4441 attgtaaaat gtaactgtat tcagcgatga cgaaattctt agctattgta atactctaga
     4501 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     4561 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     4621 ttctaattgt ttgtgtattt tagattccaa cctatggaac ttatgaatgg gagcagtggt
     4681 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     4741 aggctactgc tgactctcaa cattctactc tcaaaagaag agaaaggtag agacccaagg
     4801 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     4861 cttgctttgc tatttacaac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     4921 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
     4981 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
     5041 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
     5101 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5161 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     5221 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5281 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5341 ctggatcccc aggaagctcc tctgtgtcct cataaaccct aacctcctct acttgagagg
     5401 acattccaat cataggctgc ccatccaccc tctgtgtcct cctgttaatt aggtcactta
     5461 acaaaaagga aattgggtag gggtttttca cagaccgctt tctaaggggt aattttaaaa
     5521 tatctgggaa gtcccttcca ctgctgtgtt ccagaagtgt tggtaaacag cccacaaatg
     5581 tcaacagcag aaacatacaa gctgtcactt tgcacaaagg gcctcgtgat acgcctattt
     5641 ttataggtta atgtcatgat aataatggtt tctta
//
