LOCUS       .                       3265 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        2..940
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             992..1009
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1089..1335
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        1350..1761
                     /label="TEF1 promoter"
                     /note="promoter for EF-1α"
     promoter        1769..1816
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             1835..2206
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      2275..2522
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2597..3185)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atggaattcg ctagcgtcga
      961 ccatatgggg cccgcatgct ctagaggtac ccatcatcat catcatcatt gagtttgtag
     1021 ccttagacat gactgttcct cagttcaagt tgggcactta cgagaagacc ggtcttgcta
     1081 gattctaatc aagaggatgt cagaatgcca tttgcctgag agatgcaggc ttcatttttg
     1141 atactttttt atttgtaacc tatatagtat aggatttttt ttgtcatttt gtttcttctc
     1201 gtacgagctt gctcctgatc agcctatctc gcagctgatg aatatcttgt ggtaggggtt
     1261 tgggaaaatc attcgagttt gatgtttttc ttggtatttc ccactcctct tcagagtaca
     1321 gaagattaag tgagaccttc gtttgtgcgg atcccccaca caccatagct tcaaaatgtt
     1381 tctactcctt ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca
     1441 aaacacccaa gcacagcata ctaaattttc cctctttctt cctctagggt gtcgttaatt
     1501 acccgtacta aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga
     1561 aaaaggcaat aaaaattttt atcacgtttc tttttcttga aatttttttt tttagttttt
     1621 ttctctttca gtgacctcca ttgatattta agttaataaa cggtcttcaa tttctcaagt
     1681 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgttca ttagaaagaa
     1741 agcatagcaa tctaatctaa ggggcggtgt tgacaattaa tcatcggcat agtatatcgg
     1801 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     1861 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     1921 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     1981 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     2041 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     2101 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     2161 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtccgacg
     2221 gcggcccacg ggtcccaggc ctcggagatc cgtccccctt ttcctttgtc gatatcatgt
     2281 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     2341 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     2401 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     2461 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     2521 gcaagctgga gaccaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2761 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3001 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3241 gttaagggat tttggtcatg agatc
//
