LOCUS       62056_3140        2784 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pANo-2, complete sequence.
ACCESSION   MN436797
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    44..50
                     /label=SapI
                     /note="SapI"
     misc_feature    52..54
                     /label=Beta
                     /note="Beta"
     misc_feature    56..59
                     /label=A
                     /note="A"
     misc_feature    61..66
                     /label=BsaI
                     /note="BsaI"
     protein_bind    131..152
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        167..197
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(258..314)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(666..671)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    673..676
                     /label=F
                     /note="F"
     misc_feature    678..680
                     /label=Gamma
                     /note="Gamma"
     misc_feature    complement(682..688)
                     /label=SapI
                     /note="SapI"
     misc_feature    692..731
                     /label=UNSX
                     /note="UNSX"
     misc_feature    1121
                     /label=Mut T->C
                     /note="Mut T->C"
     rep_origin      complement(1178..1723)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(1815..2627)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgagctcttc agcaaggagt
       61 gagaccattg acagcgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
      301 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      481 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gacactagtg
      661 agcacggtct cacgctatac tgaagagcac cccaggatac atagattacc acaactccga
      721 gcccttccac cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      781 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      841 gggctcatga gcaaatattt tatctgaggt gcttcctcgc tcactgactc gctgcacgag
      901 gcagacctca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
      961 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     1021 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     1081 cggcgagcgg aaatggctta cgaacggggc ggagatttcc cggaagatgc caggaagata
     1141 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     1201 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     1261 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     1321 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     1381 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     1441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     1501 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     1561 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     1621 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     1681 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     1741 ggggtctgac gctcagtgga acgaaaaatc aatctaaagt atatatgagt aaacttggtc
     1801 tgacagttac cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1861 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1921 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1981 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2041 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     2101 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2161 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2221 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2281 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     2341 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2401 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2461 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2521 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2581 tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2641 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     2701 atgtacatca gagattttga gacacaacca attattgaag gcctccctaa cggggggcct
     2761 ttttttgttt ctgatctccc gctt
//
