LOCUS       62056_3115        2648 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pANe-1, complete sequence.
ACCESSION   MN436792
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    complement(44..49)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    51..54
                     /label=A
                     /note="A"
     misc_feature    56..58
                     /label=Alpha
                     /note="Alpha"
     misc_feature    complement(60..66)
                     /label=SapI
                     /note="SapI"
     protein_bind    130..151
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        166..196
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..220
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(257..313)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(314..330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    665..671
                     /label=SapI
                     /note="SapI"
     misc_feature    673..675
                     /label=Omega
                     /note="Omega"
     misc_feature    677..680
                     /label=B
                     /note="B"
     misc_feature    682..687
                     /label=BsaI
                     /note="BsaI"
     misc_feature    691..730
                     /label=UNSX
                     /note="UNSX"
     misc_feature    1120
                     /label=Mut T->C
                     /note="Mut T->C"
     rep_origin      complement(1177..1722)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(1817..2473)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2474..2577)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgaggtctca ggagaatgtg
       61 aagagcttga cagcgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      241 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
      301 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      361 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      481 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      541 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      601 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acactagtga
      661 gcacgctctt caggtatact tgagaccacc ccaggataca tagattacca caactccgag
      721 cccttccacc gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
      781 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
      841 ggctcatgag caaatatttt atctgaggtg cttcctcgct cactgactcg ctgcacgagg
      901 cagacctcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
      961 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
     1021 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     1081 ggcgagcgga aatggcttac gaacggggcg gagatttccc ggaagatgcc aggaagatac
     1141 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
     1201 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     1261 ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     1321 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     1381 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     1441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     1501 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     1561 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     1621 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     1681 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaag
     1741 gggtctgacg ctcagtggaa cgaaaaatca atctaaagta tatatgagta aacttggtct
     1801 gacagttacc ttattacgcc ccgccctgcc actcatcaca gtactgttgt aattcattaa
     1861 gcatgcggcc gacatggaag ccatcacaaa cggcatgatg aacctggatc gccagcggca
     1921 tcagcacctt gtcgccttgc gtataatatt tgcccatcgt gaaaacgggg gcgaagaagt
     1981 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     2041 caaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2101 ccacatcttg cgaatatatg tggagaaact gccggaaatc gtcgtggtat tcactccaga
     2161 gggacgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2221 agatcaccag ctcaccgtct ttcatggcca tacgaaactc cgggtgagcg ttcatcaggc
     2281 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2341 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2401 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatcccgtga
     2461 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2521 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaacc
     2581 tctagtcaca accaattatt gaaggcctcc ctaacggggg gccttttttt gtttctgatc
     2641 tcccgctt
//
