LOCUS       pANT7_nHA.        5339 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for in vitro transcription and 
            translation of a protein with an N-terminal HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pANT7_nHA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau 
            AY, Walter JC, LaBaer J.
  TITLE     Self-assembling protein microarrays.
  JOURNAL   Science 2004;305:86-90.
  PUBMED    15232106
REFERENCE   2  (bases 1 to 5339)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5339)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2004"; volume: "305"; pages: "86-90"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    53..514
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             516..518
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             531..557
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    567..691
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        716..746
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             800..1456
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1801..2103
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2147..2271)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2301..2318
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      2395..2442
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(2642..3230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3404..4261)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4262..4366)
                     /label=AmpR promoter
     rep_origin      complement(4700..5155)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5300..5316
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5323..5339
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaatta attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
       61 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
      121 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
      181 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
      241 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
      301 acgttgtgag ttggatagtt gtggaaagag tcaaatggct cacctcaagc gtattcaaca
      361 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
      421 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
      481 ggacgtggtt ttcctttgaa aaacacgatg ataatatgga tcggatccag tacccctacg
      541 acgtgcccga ctacgccgtg tcatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt
      601 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
      661 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
      721 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
      781 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      841 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      901 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      961 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     1021 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     1081 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     1141 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1201 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     1261 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1321 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1381 cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1441 gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc
     1501 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     1561 gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     1621 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     1681 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     1741 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa
     1801 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     1861 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     1921 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     1981 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     2041 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     2101 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
     2161 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     2221 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatgaaca
     2281 agcttgcggc cgcactcgag caccaccacc accaccactg agatntgagc tgactgaaaa
     2341 aaaaaaaaaa aaaaaaaaaa aaaaaagttt aaacactagt ccgctgagca ataactagca
     2401 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     2461 tccgggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3061 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     4441 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     4501 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     4561 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     4621 agcagattgt actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag
     4681 gagaaaatac cgcatcagga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     4741 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     4801 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     4861 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     4921 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     4981 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     5041 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     5101 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcgcgc
     5161 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     5221 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     5281 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactata
//
