LOCUS       40924_4529        6342 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pANT1202, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Characterization of the Streptomyces sp. strain C5 snp locus and 
            development of snp-derived expression vectors
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1647-1654 (2003)
  PUBMED    12620855
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2001) School of Dentistry, Case Western Reserve 
            University, 2123 Abington Rd, Cleveland, OH 44106, USA
REFERENCE   3  (bases 1 to 6342)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6342)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2003"; volume: "69"; issue: "3"; pages:
            "1647-1654"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-DEC-2001) School of Dentistry, Case Western Reserve University, 
            2123 Abington Rd, Cleveland, OH 44106, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(191..1561)
                     /codon_start=1
                     /product="replication protein pIJ101 Rep"
                     /label=replication protein pIJ101 Rep
                     /protein_id="AAL61989.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDAAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     primer_bind     2846..2862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2917..3681)
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     primer_bind     complement(3872..3888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3896..3912)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3920..3950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3965..3986)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4274..4862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4967..5097
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      complement(5097..5137)
                     /label=snpA promoter
                     /note="snpA promoter"
                     /regulatory_class="promoter"
     CDS             5355..6296
                     /codon_start=1
                     /product="LysR-like transcriptional activator SnpR"
                     /label=LysR-like transcriptional activator SnpR
                     /protein_id="AAL61991.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"
ORIGIN
        1 ctgggggggg acggcggtgc gggggatgca gcgggtcggc ggtaccgcct gcacgaagtg
       61 ccggcggggc cgaccccggg cgagtaatcc caggattact cccgcggctt cgaccccggc
      121 cgccgtcgcc gcgtacgtca ccgacccccg ccgtacgtca ccgggatgac gtacggcggg
      181 ggggagcgag ttagtgcgaa gtgggcccac ttgcgagccg ggcgatgtgc cgggcggccc
      241 gctcctggcg gtcgtcggcg tcgtcgtcct ggtcgtcgtc ctgctctcgc cgtcggcgtg
      301 cagttgcttc ctcgcggcgc tgggcgaggg cggcgagcat gtcggcgtac gcctcggcca
      361 cctcccccgc cgtgagcacc accactgtgt cggccgcgtc ggccagcgcc aggacctccc
      421 gcacccgttc gcccacggcc gccgcatcct cgttgccgtc cttgccttcg gcggcccggg
      481 tcgcctcgag gtcgagggcg cggcgggtga ccgcgtgcca tccgtcctcg gtcacggcga
      541 ccccggcccg cagctccccg ccgtcggcgt cggccgccag gagcagatcg aggtcgtcgg
      601 cctcggtgtc gccgccgtcg agcccgagca tctgccgcag gtagcgggtc cattcgatgg
      661 cccggcgtcc ccgggttgcc cgctcgtact cgtgccagcg cgagaggttc cactccagcg
      721 agccgacccc ggcggcgtcg tcctcggtca tgccgccggt caggtccccg atccgtccga
      781 ggagttcgaa cggggcgacg ttcccgccgg tcgccgtctt gaggtcggcg cgggcgagtt
      841 cgagggcggg cgccttcccg tcctgggtct tggcgatgta ctcggcgagg tcgttggcgt
      901 cgcgctcggt ctccagccgc ttgaagtcga cgccgtgccg gtcgtcgggc gtgaaggcgg
      961 ggttgacctt gcgcagggcg gcggtccaca cggaccgcca gtgcccctgc cactcgtcga
     1021 gcgcggcgcc ggtcggctcg aaggtggcga cgatctgctt cgcggaccgc tccccctcgg
     1081 tccggccgcc gaccaggacg atcgcgtgga tgtgcgggtg ccagccgttg atctgcccca
     1141 cggtgacttc ggtcgcgcgg atcatgccga cgtacccgat ccggtctcgg atgccctcgc
     1201 ggtcggcggc ccggtgcccg tccttggccc ggcgtccggc ccacgtgccg cccgtgatca
     1261 gtcgctggta ggcgcccggc cgccgggggc tgtccggcgt cttccgggtg ccctggaggg
     1321 cgtccatgag gtccgcgagc cggtccgtgt gcccatggcg ggccgtgaag gtgaccaggt
     1381 aggcggtccc cccgcgcttg atccactcga ccacggcggc ggtgatctcc tcggcccgct
     1441 tgtgccggat cgtggcggcg cagaccgggc agagccagat ccgcccgcac cgcatcaggc
     1501 ccaggaccac ggacgttccg gccgccgtct gggcgacgat cacgccggag gcagggtcca
     1561 tcagggcgcg gccgcagccc ttgcacgcgg cgtccccgct gatccgccac agcgtccggc
     1621 ggcggctgta ccgggcggct ttccgcagtc gggcagcctc ggtccgcgac gtgcttccta
     1681 cttcccagag gctgtcgcct ctcgggctct ccccatccac cccgtccgga gaaaccgcag
     1741 gtcggagggg tgcgggaaac tctgttgttt ctttcccaag gtgttcgctt ttgcctcggg
     1801 cggcatctcg cgtcacacgc gcgatcgccc gcttcgctgc catccggcag cggtctgagc
     1861 agtagatacg cggccgtttg cccggtgtgt gggcaattgc ggtcccgcag tggcagcggg
     1921 gcccggcggg ccgatctggc aatgcctcgg catcgctccg tactctgggc acgagcaacg
     1981 ttcctgtctc gcccggctaa ggggcgcgag tctgggagcg gacgggtcgg aggtgcgaag
     2041 tccggcccgt tgctctttgg tctggtggga atcctggcac caatcgggcc agaggttccc
     2101 tccgccactc ccgacgcccc ttggggctgg tgtgacttgg agggccgaag agagccccgc
     2161 cgggtatccg gcggggcttt gacgtgcggt cagtgcgtgt gtcggcgagc gatggccacg
     2221 aggccctgga agccgagcgg tccggcgaag tcggcccagt cgcaaccggg ctcagcgcag
     2281 tgggcggacc agccaccgcc gttggggtcc tggaccaggt tcacggtccc ctcggtcagg
     2341 cgtccgtcga agtcggtcat ggtcggtctc ctggtgggtg ggggcggggc gccagcacga
     2401 agtgccggcg ccccgcgggg gttggtcggg tcaggcgccg aaccggcggg cggcggcggc
     2461 gaccaggccg tcggcggcgg ccatggcgcg gtcgcggtcg gtggtgaggg cggtgcggtc
     2521 ggcggcggcc cagtcctgct ctcacgcaac gtctacgtgg acgctcaggg cgacacgatc
     2581 gaggtcgggg agtccgtcgc tccggcgggc cgctggcgcg tccaccggga ctgatcaagg
     2641 cgaatacttc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     2701 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     2761 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     2821 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattaat tcgagctcag
     2881 ccaatcgact ggcgagcggc atcgcattct tcgcatcccg ccctctggcg gatgcaggaa
     2941 gatcaacgga tctcggccca gttgacccag ggctgtcgcc acaatgtcgc gggagcggat
     3001 caaccgagca aaggcatgac cgactggacc ttccttctga aggctcttct ccttgagcca
     3061 cctgtccgcc aaggcaaagc gctcacagca gtggtcattc tcgagataat cgacgcgtac
     3121 caacttgcca tcctgaagaa tggtgcagtg tctcggcacc ccatagggaa cctttgccat
     3181 caactcggca agatgcagcg tcgtgttggc atcgtgtccc acgccgagga gaagtacctg
     3241 cccatcgagt tcatggacac gggcgaccgg gcttgcaggc gagtgaggtg gcaggggcaa
     3301 tggatcagag atgatctgct ctgcctgtgg ccccgctgcc gcaaaggcaa atggatgggc
     3361 gctgcgcttt acatttggca ggcgccagaa tgtgtcagag acaactccaa ggtccggtgt
     3421 aacgggcgac gtggcaggat cgaacggctc gtcgtccaga cctgaccacg agggcatgac
     3481 gagcgtccct cccggaccca gcgcagcacg cagggcctcg atcagtccaa gtggcccatc
     3541 ttcgaggggc cggacgctac ggaaggagct gtggaccagc agcacaccgc cgggggtaac
     3601 cccaaggttg agaagctgac cgatgagctc ggcttttcgc cattcgtatt gcacgacatt
     3661 gcactccacc gctgatgaca tcagtcgatc atagcacgat caacggcact gttgcaaata
     3721 gtcggtggtg ataaacttat catccccttt tgctgatgga gctgcacatg aacccattca
     3781 aaggccggca ttttcagcgt gacatcattc tgtgggccgt acgctggtac tgcaaatacg
     3841 gcatcagtta ccgtgagctg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4681 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaagctcc
     4981 atggacccgt gcaggggccc cgacacggtc ggggcccctg cactctatct attcacgcgt
     5041 ggtaccacta gtaagcttgg atcctgcaga gctctagatc tgaattcgca tgcgcatcga
     5101 tgactcctcg aggtgggggg atgagtcaga catcgtgagt gtccgtgccc gtacggccgt
     5161 tgtgatgatg gcaaccgccg atagggccgc cctatccccc tccgtcccca cctgccccgc
     5221 cctgcccggc ggccccggcc ggggcccctc ccgatccggg ccgacgcctc gcgccgtgaa
     5281 ggcccgatgg tcctgatcgg tcgccatttt gtatgtctgg tgaaagctga acttccgcct
     5341 taccctccca ggacatggag cttgaggtca ggcacctcag ggcgctgtgc gccatcgccg
     5401 acaccggcag cctgcaccgc gcggcacgcc aactgggagt gacacagccc tcgttgagca
     5461 cgcagctgcg gcgcatcgaa cacgagctgg gcggtgccct gttcgtccgg gcccgcaccg
     5521 gctgccgccc cacaccgctg ggccggctgg tgctcagtcg tgcccgcccc ctggtggccg
     5581 aattgcgctc cctcgtcagc gaggcccgcg ccgccgccgt cggcggacgc cagctgcgcg
     5641 tcggctccac ggccagccgg gccctggcgg gctggctgcg ccggctccgc cggcactggc
     5701 aggaacccac cctgcacatg gacgtctccg ccaacgccct gctgcgcatg gtggccgacg
     5761 gccacctcga cgtcgccttc gtgcacgagg tcgagggcag cccgctgcgc gtccccgaag
     5821 ggctccgggt ccgcgtactg gtccagcggg aaccgcagtt cgtctgcctg ccggccgacc
     5881 acccggccgc ggcgaagccg tcgtacgcct cgccgacctg gcccacgacc cgctggatga
     5941 tcgaccccac cgtcgacggc gagtgggacg cggtgcgccg ggtcctgcgc gccgagggac
     6001 tcgacccgcg catcctgcac ggggactacc acaccgccgc gtccctggtc gccaccggcg
     6061 aggtcgtcac cgtggtccag ccgacctcgc cctcccgcgc cgagacggcc gtccgccggc
     6121 tgcacggcga cccgctcggc gtacggctgc tgctggcggc ccgcacggac acggaactgg
     6181 agggcgtcta ccccgacctc gcggaggcct acggggaggt cgcccggcag gccccggcgt
     6241 accgggagtg gctggaacgc agtgggtcgg gggcactcgt cccagccctc ccgtgacgac
     6301 cgcgtacccc ggcccccttc ccgtaacccg acgggagatg cc
//
