LOCUS       40924_4509        7043 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pANFluc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire 
            properties of cardiomyocytes after transduction with a recombinant 
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
  PUBMED    17579192
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 7043)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7043)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      37..2278
                     /label=Homo sapiens ANP
                     /note="Homo sapiens ANP"
                     /regulatory_class="promoter"
     CDS             2313..3962
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(4006..4127)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4546..5134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5308..6165)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6166..6270)
                     /label=AmpR promoter
     rep_origin      6297..6752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6883..6931
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6945..7036
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tggccagcca ggacagagct
       61 ggtcactcaa cacacatttt ctgagcttct aaggagtgga ttaaaacaca ttcggtgtag
      121 aaatagatca ggagagcagc caacacacac agacttggta agtgcacaaa aaagctacag
      181 ccccaggggc atggggaagg tgaaggagct cccttggggc atctgaaaga tggggaatgg
      241 gggtttaatt ggggaatgca gctaagaggt aaggtggcag ctctctacgt gaaataggct
      301 aggatacaga catcagcgtc agacctgggg atccagtcct ggttccgcca cttaggcacc
      361 tagtggcctt gggcaagtgg catagcctct cgggctcagt tctgttccct tgtaagcaag
      421 gacaatgcta tctaccacct ggggatggtg atcaaaataa aatcatttcc ataaagttct
      481 cagcacagtg ctaggcatgc tacaagcaac caataaagac aactatgatg gccatcttgc
      541 agggactcag cactgcacac ttgttatgtg ttgacacgtt ctcagctcac ttgggctcca
      601 caacaatcct gcaaggtagg cagggaagat ttctacttta ttcattcatt tatttattta
      661 ttttttgaga ccgagtccca ctctgttgcc caggctagag tgcagtggtg cgatcttggt
      721 tcactacaac ctccgcctcc caaggtcaag caattctttt acctcagcct ccctagtagc
      781 ggggattaca ggcacatgcc accacaccca gctaattttt atatttttag tagagacaga
      841 gttttgccat gttggtaggg ctggtctcaa actcctggcc tcaggtgatc cacccacctt
      901 agcctcccaa agtgctggga ttatacgtgt gagccactgc acccggccgg tttttaacat
      961 tctttctcat tttacacaca aggaaagtaa ggctgcgaag aggtatgcaa ctggtttgtt
     1021 ttgggctaca gtactggtct ctcagccctt caaatttaga gttttccccc agtatacgtt
     1081 gcattgaatg gtaatggcta acactgagtt cagtgcttag caaacaggcc attcattaaa
     1141 catagacaga cttgctgcct gttatttccc ttttgggggc cagggaaatt gtgaccttgg
     1201 tggctcggtc acttgccact tgccacgtcc tcagggaagg atgtagaagg cattgaggga
     1261 aactgactat acctccttca cccccacctc ccttcctcca tcggtcaagt tgccacgggc
     1321 acaagcccag gaagggcagg gggatgttaa caaacttcag acacgaagag tctgagcatc
     1381 tataaacagc aacggaagaa atgaaattgg ctgcgtcctc taagcctgtc cccgcagcat
     1441 gctggaggag ggtcgcgggg gacatggaag aggaggagct ttggagagag gatgcttgtg
     1501 ctcccccgcc ttttcttgct atttctattt gggggttgga ttcctgggag cttcatcaca
     1561 tttggttctc agctgacttt atatactaaa aaataacttc ctttcgcctg accatggaga
     1621 gggactgcca ggggtgaagg cagccctgtc tgaggccaga ggtctgccca cgtggcggat
     1681 gaggcaggtg tgaggccagc ttgagcatct ggatccattt gtctcgggct gctggctgcc
     1741 tgccatttcc tcctctccac ccttatttgg aggccctgac agctgagcca caaacaaacc
     1801 aggggagctg ggcaccagcc aagcgtcacc ctctgtttcc ccgcacgggt accagcgtcg
     1861 aggagaaaga atcctgaggc acggcggtga gataaccaag gactcttttt tactcttctc
     1921 acacctttga agtgggagcc tcttgagtca aatcagtaag aatgcggctc ttgcagctga
     1981 gggtctgggg ggctgttggg gctgcccaag gcagagaggg gctgtgacaa gccctgcggg
     2041 atgataactt taaaagggca tctcctgctg gcttctcact tggcagcttt atcactgcaa
     2101 gtgacagaat ggggagggtt ctgtctctcc tgcgtgcttg gagagctggg gggctataaa
     2161 aagaggcggc actgggcagc tgggagacag ggacagacgt aggccaagag aggggaacca
     2221 gagaggaacc agaggggaga gacagagcag caagcagtgg attgctcctt gacgacgaag
     2281 cttggcattc cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag
     2341 gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta
     2401 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg
     2461 acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat
     2521 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta
     2581 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata
     2641 atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca
     2701 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta
     2761 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat
     2821 ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca
     2881 agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc
     2941 tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc
     3001 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt
     3061 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag
     3121 aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa
     3181 ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac
     3241 acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga
     3301 ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc
     3361 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg
     3421 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac
     3481 tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg
     3541 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg
     3601 aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg
     3661 ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg
     3721 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg
     3781 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga
     3841 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac
     3901 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg
     3961 tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     4021 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4081 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4141 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     4201 acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc
     4261 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     4321 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     4381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4741 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4981 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     6301 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6361 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6421 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6481 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6541 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6601 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6661 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6721 gaattttaac aaaatattaa cgtttacaat ttcccattcg ccattcaggc tgcgcaactg
     6781 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata
     6841 agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt
     6901 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat
     6961 caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt
     7021 gccagaacat ttctctatcg ata
//
