LOCUS       Exported                6760 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Vector with a hygromycin B resistance marker for transforming 
            Aspergillus species.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CA.
  TITLE     Transformation of Aspergillus based on the hygromycin B resistance 
            marker from Escherichia coli.
  JOURNAL   Gene 1987;56:117-24.
  PUBMED    2824287
REFERENCE   2  (bases 1 to 6760)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6760)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "56"; pages: "117-24"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     The trpC terminator region was corrected based on the updated 
            sequence record X02390.
FEATURES             Location/Qualifiers
     source          1..6760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2129
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     intron          2178..2293
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2302..3318
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     terminator      3337..4112
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene (Punt et al., 1987)"
     primer_bind     complement(4135..4151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4625..4729
                     /label=AmpR promoter
     CDS             4730..5587
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5761..6349
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6637..6658
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6673..6703
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6711..6727
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6735..6751
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattccctt gtatctctac acacaggctc aaatcaataa gaagaacggt tcgtcttttt
       61 cgtttatatc ttgcatcgtc ccaaagctat tggcgggata ttctgtttgc agttggctga
      121 cttgaagtaa tctctgcaga tctttcgaca ctgaaatacg tcgagcctgc tccgcttgga
      181 agcggcgagg agcctcgtcc tgtcacaact accaacatgg agtacgataa gggccagttc
      241 cgccagctca ttaagagcca gttcatgggc gttggcatga tggccgtcat gcatctgtac
      301 ttcaagtaca ccaacgctct tctgatccag tcgatcatcc gctgaaggcg ctttcgaatc
      361 tggttaagat ccacgtcttc gggaagccag cgactggtga cctccagcgt ccctttaagg
      421 ctgccaacag ctttctcagc cagggccagc ccaagaccga caaggcctcc ctccagaacg
      481 ccgagaagaa ctggaggggt ggtgtcaagg aggagtaagc tccttattga agtcggagga
      541 cggagcggtg tcaagaggat attcttcgac tctgtattat agataagatg atgaggaatt
      601 ggaggtagca tagcttcatt tggatttgct ttccaggctg agactctagc ttggagcata
      661 gagggtcctt tggctttcaa tattctcaag tatctcgagt ttgaacttat tccctgtgaa
      721 ccttttattc accaatgagc attggaatga acatgaatct gaggactgca atcgccatga
      781 ggttttcgaa atacatccgg atgtcgaagg cttggggcac ctgcgttggt tgaatttaga
      841 acgtggcact attgatcatc cgatagctct gcaaagggcg ttgcacaatg caagtcaaac
      901 gttgctagca gttccaggtg gaatgttatg atgagcattg tattaaatca ggagatatag
      961 catgatctct agttagctca ccacaaaagt cagacggcgt aaccaaaagt cacacaacac
     1021 aagctgtaag gatttcggca cggctacgga agacggagaa gccaccttca gtggactcga
     1081 gtaccattta attctatttg tgtttgatcg agacctaata cagcccctac aacgaccatc
     1141 aaagtcgtat agctaccagt gaggaagtgg actcaaatcg acttcagcaa catctcctgg
     1201 ataaacttta agcctaaact atacagaata agataggtgg agagcttata ccgagctccc
     1261 aaatctgtcc agatcatggt tgaccggtgc ctggatcttc ctatagaatc atccttattc
     1321 gttgacctag ctgattctgg agtgacccag agggtcatga cttgagccta aaatccgccg
     1381 cctccaccat ttgtagaaaa atgtgacgaa ctcgtgagct ctgtacagtg accggtgact
     1441 ctttctggca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagccactg
     1501 gccagacagc tctggcggct ctgaggtgca gtggatgatt attaatccgg gaccggccgc
     1561 ccctccgccc cgaagtggaa aggctggtgt gcccctcgtt gaccaagaat ctattgcatc
     1621 atcggagaat atggagcttc atcgaatcac cggcagtaag cgaaggagaa tgtgaagcca
     1681 ggggtgtata gccgtcggcg aaatagcatg ccattaacct aggtacagaa gtccaattgc
     1741 ttccgatctg gtaaaagatt cacgagatag taccttctcc gaagtaggta gagcgagtac
     1801 ccggcgcgta agctccctaa ttggcccatc cggcatctgt agggcgtcca aatatcgtgc
     1861 ctctcctgct ttgcccggtg tatgaaaccg gaaaggccgc tcaggagctg gccagcggcg
     1921 cagaccggga acacaagctg gcagtcgacc catccggtgc tctgcactcg acctgctgag
     1981 gtccctcagt ccctggtagg cagctttgcc ccgtctgtcc gcccggtgtg tcggcggggt
     2041 tgacaaggtc gttgcgtcag tccaacattt gttgccatat tttcctgctc tccccaccag
     2101 ctgctctttt cttttctctt tcttttccca tcttcagtat attcatcttc ccatccaaga
     2161 acctttattt cccctaagta agtactttgc tacatccata ctccatcctt cccatccctt
     2221 attcctttga acctttcagt tcgagctttc ccacttcatc gcagcttgac taacagctac
     2281 cccgcttgag cagacatcac catgcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     2341 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     2401 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     2461 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     2521 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     2581 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     2641 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     2701 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     2761 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     2821 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     2881 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     2941 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3001 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     3061 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     3121 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     3181 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     3241 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     3301 cgtccgaggg caaaggaata gagtagatgc cgaccgggat ccacttaacg ttactgaaat
     3361 catcaaacag cttgacgaat ctggatataa gatcgttggt gtcgatgtca gctccggagt
     3421 tgagacaaat ggtgttcagg atctcgataa gatacgttca tttgtccaag cagcaaagag
     3481 tgccttctag tgatttaata gctccatgtc aacaagaata aaacgcgttt cgggtttacc
     3541 tcttccagat acagctcatc tgcaatgcat taatgcattg gacctcgcaa ccctagtacg
     3601 cccttcaggc tccggcgaag cagaagaata gcttagcaga gtctattttc attttcggga
     3661 gacgagatca agcagatcaa cggtcgtcaa gagacctacg agactgagga atccgctctt
     3721 ggctccacgc gactatatat ttgtctctaa ttgtactttg acatgctcct cttctttact
     3781 ctgatagctt gactatgaaa attccgtcac cagcccctgg gttcgcaaag ataattgcac
     3841 tgtttcttcc ttgaactctc aagcctacag gacacacatt catcgtaggt ataaacctcg
     3901 aaaatcattc ctactaagat gggtatacaa tagtaaccat gcatggttgc ctagtgaatg
     3961 ctccgtaaca cccaatacgc cggccgaaac ttttttacaa ctctcctatg agtcgtttac
     4021 ccagaatgca caggtacact tgtttagagg taatccttct ttctagaagt cctcgtgtac
     4081 tgtgtaagcg cccactccac atctccactc gacctgcagg catgcaagct tggcactggc
     4141 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4201 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4261 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     4321 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     4381 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4501 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4681 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4801 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6421 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6481 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6541 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     6601 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     6661 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     6721 ataacaattt cacacaggaa acagctatga ccatgattac
//
