LOCUS       40924_4489        5274 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pAMPAT-MCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Lipka V, Rademacher T, Panstruga R.
  TITLE     pAMPAT-MCS binary vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Lipka V, Rademacher T, Panstruga R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2003) Plant-Microbe Interaction, 
            Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg
            10, Koeln D-50829, Germany
REFERENCE   3  (bases 1 to 5274)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5274)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-OCT-2003) Plant-Microbe Interaction, Max-Planck-Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(95..347)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(370..918)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(961..1140)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1284..1957
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     1960..1976
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2010..2026)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2309..2333
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      2445..3154
                     /label=oriV
                     /note="incP origin of replication"
     promoter        3228..3319
                     /label=AmpR promoter
     CDS             3320..4177
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4351..4939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5251..5274
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatga tcgaatacta
       61 acgtctctac cagatatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
      121 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
      181 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
      241 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
      301 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
      361 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
      421 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
      481 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
      541 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
      601 gagcgtggag cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc
      661 cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc
      721 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
      781 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
      841 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
      901 gcgtcgttct gggctcatgg tagatccccc tcgatcgagt tgagagtgaa tatgagactc
      961 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
     1021 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
     1081 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc
     1141 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
     1201 cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcggtttg ggcgcgcccg
     1261 gtctcagaag accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     1321 ctcggattcc attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat
     1381 ggcttctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga atgcctctac
     1441 cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa aagaagacgt
     1501 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct
     1561 cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta ttgagacttt
     1621 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     1681 catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg
     1741 aaaggctatc gttcaagaat gcctctaccg acagtggtcc caaagatgga cccccaccca
     1801 cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     1861 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     1921 cctctatata aggaagttca tttcatttgg agaggacctc gaggtcgacg gtatcgataa
     1981 gcttgatatc gaattcctgc agcccggggg atccactagt tctagagtcc gcaaaaatca
     2041 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     2101 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     2161 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2221 aaccaaaatc cagtgaccgg gcggccgcca ccgcggtgga gggggatcag attgtcgttt
     2281 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     2341 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     2401 gtccatttgt atgtgtacat caccgacgag caaggcaaga ccgagcgcct ttccgacgct
     2461 caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc
     2521 agaaacgccg tcgaagccgt gtgcgagaca ccggccgccg gcgttgtgga tacctcgcgg
     2581 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     2641 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     2701 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     2761 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     2821 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     2881 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     2941 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
     3001 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
     3061 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
     3121 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
     3181 atcggggcag taacgggatg ggcgatcagc ccgacaagct acccctattt gtttattttt
     3241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3421 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3661 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3721 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3781 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3841 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3901 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3961 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4021 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4081 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4141 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4981 ctggcctttt gctcacatgg actctagcta gaggatcaca ggcagcaacg ctctgtcatc
     5041 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
     5101 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
     5161 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
     5221 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaa
//
