LOCUS       62056_3085        4347 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_50opt, complete sequence.
ACCESSION   MK606515
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(1610..2266)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2267..2369)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2895..3440)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3627..4337)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccactaa accgactgat ggtaatggtc ctactaaacc tacggatggt aatggcccga
      181 cgaagccgac tgacggtaat ggccctacta aaccaactga tggcaacggt ccaactaagc
      241 ctaccgacgg taacggtcca acgaaaccga ctgacggcaa tggcccaact aaacctacgg
      301 acggcaatgg tccaaccaaa cctaccgatg gtaacggccc aaccaaaccg accgacggca
      361 acggtccgac caagccgacc gacggtaatg gtccgaccaa accaaccgat ggcaacggcc
      421 ctaccaagcc gactgatggc aatggtccga cgaaacctac tgacggtaac ggcccgacga
      481 aaccgacgga cggcaacggc cctactaaac cgactgatgg taatggtcct actaaaccta
      541 cggatggtaa tggcccgacg aagccgactg acggtaatgg ccctactaaa ccaactgatg
      601 gcaacggtcc aactaagcct accgacggta acggtccaac gaaaccgact gacggcaatg
      661 gcccaactaa acctacggac ggcaatggtc caaccaaacc taccgatggt aacggcccaa
      721 ccaaaccgac cgacggcaac ggtccgacca agccgaccga cggtaatggt ccgaccaaac
      781 caaccgatgg taatggtccc actaaaccga ctgatggtaa tggtcctact aaacctacgg
      841 atggtaatgg cccgacgaag ccgactgacg gtaatggccc tactaaacca actgatggca
      901 acggtccaac taagcctacc gacggtaacg gtccaacgaa accgactgac ggcaatggcc
      961 caactaaacc tacggacggc aatggtccaa ccaaacctac cgatggtaac ggcccaacca
     1021 aaccgaccga cggcaacggt ccgaccaagc cgaccgacgg taatggtccg accaaaccaa
     1081 ccgatggcaa cggccctacc aagccgactg atggcaatgg tccgacgaaa cctactgacg
     1141 gtaacggccc gacgaaaccg acggacggca acggccctac taaaccgact gatggtaatg
     1201 gtcctactaa acctacggat ggtaatggcc cgacgaagcc gactgacggt aatggcccta
     1261 ctaaaccaac tgatggcaac ggtccaacta agcctaccga cggtaacggt ccaacgaaac
     1321 cgactgacgg caatggccca actaaaccta cggacggcaa tggtccaacc aaacctaccg
     1381 atggtaacgg cccaaccaaa ccgaccgacg gcaacggtcc gaccaagccg accgacggta
     1441 atggtccgac caaaccaacc gatggtaatg gtcccagaag agctaagtaa gtaagagctc
     1501 gacccggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     1561 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     1621 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1681 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1741 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1801 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     1861 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1921 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1981 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2041 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2101 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2161 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2221 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2281 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2341 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     2401 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     2461 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     2521 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     2581 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt
     2641 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     2701 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     2761 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     2821 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     2881 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     2941 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     3001 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     3061 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     3121 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     3181 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     3241 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     3301 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     3361 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     3421 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     3481 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     3541 aattctcatg tttgacagca tgccatcgat cgcgatcttg ctcaattgtt atcagctatg
     3601 cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata
     3661 actttcccca caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt
     3721 ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc
     3781 ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt
     3841 aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca
     3901 acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca
     3961 tcacctttgg taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa
     4021 acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg
     4081 tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca
     4141 agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct
     4201 gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc
     4261 tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt
     4321 ttcttttttg tgctcatacg ttaaatc
//
