LOCUS       62056_3080        3834 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_30, complete sequence.
ACCESSION   MK606513
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(1097..1753)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1754..1856)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2382..2927)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3114..3824)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccaccaa accgaccgac ggtaacgggc ccaccaaacc gaccgacggt aacgggccca
      181 ccaaaccgac cgacggtaac gggcccacca aaccgaccga cggtaacggg cccaccaaac
      241 cgaccgacgg taacgggccc accaaaccga ccgacggtaa cgggcccacc aaaccgaccg
      301 acggtaacgg gcccaccaaa ccgaccgacg gtaacgggcc caccaaaccg accgacggta
      361 acgggcccac caaaccgacc gacggtaacg ggcccaccaa accgaccgac ggtaacgggc
      421 ccaccaaacc gaccgacggt aacgggccca ccaaaccgac cgacggtaac gggcccacca
      481 aaccgaccga cggtaacggg cccaccaaac cgaccgacgg taacgggccc accaaaccga
      541 ccgacggtaa cgggcccacc aaaccgaccg acggtaacgg gcccaccaaa ccgaccgacg
      601 gtaacgggcc caccaaaccg accgacggta acgggcccac caaaccgacc gacggtaacg
      661 ggcccaccaa accgaccgac ggtaacgggc ccaccaaacc gaccgacggt aacgggccca
      721 ccaaaccgac cgacggtaac gggcccacca aaccgaccga cggtaacggg cccaccaaac
      781 cgaccgacgg taacgggccc accaaaccga ccgacggtaa cgggcccacc aaaccgaccg
      841 acggtaacgg gcccaccaaa ccgaccgacg gtaacgggcc caccaaaccg accgacggta
      901 acgggcccac caaaccgacc gacggtaacg ggcccaccaa accgaccgac ggtaacgggc
      961 ccagaagagc taagtaagta agagctcgac ccggtcgaat ttgctttcga atttctgcca
     1021 ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg caccaataac
     1081 tgccttaaaa aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1141 gcattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc gccagcggca
     1201 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1261 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1321 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1381 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1441 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1501 atatcaccag ctcaccgtct ttcattgcca tacggaattc cggatgagca ttcatcaggc
     1561 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1621 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1681 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1741 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1801 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     1861 ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta
     1921 tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg
     1981 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
     2041 gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
     2101 accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg
     2161 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
     2221 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     2281 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     2341 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     2401 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     2461 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     2521 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     2581 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     2701 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     2761 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     2821 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     2881 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     2941 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     3001 aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcatgc catcgatcgc
     3061 gatcttgctc aattgttatc agctatgcgc cgaccagaac accttgccga tcagccaaac
     3121 gtctcttcag gccactgact agcgataact ttccccacaa cggaacaact ctcattgcat
     3181 gggatcattg ggtactgtgg gtttagtggt tgtaaaaaca cctgaccgct atccctgatc
     3241 agtttcttga aggtaaactc atcaccccca agtctggcta tgcagaaatc acctggctca
     3301 acagcctgct cagggtcaac gagaattaac attccgtcag gaaagcttgg cttggagcct
     3361 gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagaatc actggctttt
     3421 ttggttgtgc ttacccatct ctccgcatca cctttggtaa aggttctaag ctcaggtgag
     3481 aacatccctg cctgaacatg agaaaaaaca gggtactcat actcacttct aagtgacggc
     3541 tgcatactaa ccgcttcata catctcgtag atttctctgg cgattgaagg gctaaattct
     3601 tcaacgctaa ctttgagaat ttttgcaagc aatgcggcgt tataagcatt taatgcattg
     3661 atgccattaa ataaagcacc aacgcctgac tgccccatcc ccatcttgtc tgcgacagat
     3721 tcctgggata agccaagttc atttttcttt ttttcataaa ttgctttaag gcgacgtgcg
     3781 tcctcaagct gctcttgtgt taatggtttc ttttttgtgc tcatacgtta aatc
//
