LOCUS       62056_3075        3672 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_25opt, complete sequence.
ACCESSION   MK606514
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(935..1591)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1592..1694)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2220..2765)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2952..3662)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccactaa accgactgat ggtaatggtc ctactaaacc tacggatggt aatggcccga
      181 cgaagccgac tgacggtaat ggccctacta aaccaactga tggcaacggt ccaactaagc
      241 ctaccgacgg taacggtcca acgaaaccga ctgacggcaa tggcccaact aaacctacgg
      301 acggcaatgg tccaaccaaa cctaccgatg gtaacggccc aaccaaaccg accgacggca
      361 acggtccgac caagccgacc gacggtaatg gtccgaccaa accaaccgat ggcaacggcc
      421 ctaccaagcc gactgatggc aatggtccga cgaaacctac tgacggtaac ggcccgacga
      481 aaccgacgga cggcaacggc cctactaaac cgactgatgg taatggtcct actaaaccta
      541 cggatggtaa tggcccgacg aagccgactg acggtaatgg ccctactaaa ccaactgatg
      601 gcaacggtcc aactaagcct accgacggta acggtccaac gaaaccgact gacggcaatg
      661 gcccaactaa acctacggac ggcaatggtc caaccaaacc taccgatggt aacggcccaa
      721 ccaaaccgac cgacggcaac ggtccgacca agccgaccga cggtaatggt ccgaccaaac
      781 caaccgatgg taatggtccc agaagagcta agtaagtaag agctcgaccc ggtcgaattt
      841 gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta gcaccaggcg
      901 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt
      961 actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa
     1021 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1081 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1141 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1201 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1261 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1321 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
     1381 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1441 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1501 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1561 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1621 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1681 ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa
     1741 cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg
     1801 cgcaaagtgc gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
     1861 ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
     1921 gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact
     1981 atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc
     2041 accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     2101 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
     2161 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
     2221 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     2281 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
     2341 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     2401 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     2461 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2521 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     2581 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     2641 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
     2701 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     2761 ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct
     2821 cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgtttga
     2881 cagcatgcca tcgatcgcga tcttgctcaa ttgttatcag ctatgcgccg accagaacac
     2941 cttgccgatc agccaaacgt ctcttcaggc cactgactag cgataacttt ccccacaacg
     3001 gaacaactct cattgcatgg gatcattggg tactgtgggt ttagtggttg taaaaacacc
     3061 tgaccgctat ccctgatcag tttcttgaag gtaaactcat cacccccaag tctggctatg
     3121 cagaaatcac ctggctcaac agcctgctca gggtcaacga gaattaacat tccgtcagga
     3181 aagcttggct tggagcctgt tggtgcggtc atggaattac cttcaacctc aagccagaat
     3241 gcagaatcac tggctttttt ggttgtgctt acccatctct ccgcatcacc tttggtaaag
     3301 gttctaagct caggtgagaa catccctgcc tgaacatgag aaaaaacagg gtactcatac
     3361 tcacttctaa gtgacggctg catactaacc gcttcataca tctcgtagat ttctctggcg
     3421 attgaagggc taaattcttc aacgctaact ttgagaattt ttgcaagcaa tgcggcgtta
     3481 taagcattta atgcattgat gccattaaat aaagcaccaa cgcctgactg ccccatcccc
     3541 atcttgtctg cgacagattc ctgggataag ccaagttcat ttttcttttt ttcataaatt
     3601 gctttaaggc gacgtgcgtc ctcaagctgc tcttgtgtta atggtttctt ttttgtgctc
     3661 atacgttaaa tc
//
