LOCUS       62056_3070        3537 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_20, complete sequence.
ACCESSION   MK606512
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(800..1456)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1457..1559)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2085..2630)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2817..3527)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccaccaa accgaccgac ggtaacgggc ccaccaaacc gaccgacggt aacgggccca
      181 ccaaaccgac cgacggtaac gggcccacca aaccgaccga cggtaacggg cccaccaaac
      241 cgaccgacgg taacgggccc accaaaccga ccgacggtaa cgggcccacc aaaccgaccg
      301 acggtaacgg gcccaccaaa ccgaccgacg gtaacgggcc caccaaaccg accgacggta
      361 acgggcccac caaaccgacc gacggtaacg ggcccaccaa accgaccgac ggtaacgggc
      421 ccaccaaacc gaccgacggt aacgggccca ccaaaccgac cgacggtaac gggcccacca
      481 aaccgaccga cggtaacggg cccaccaaac cgaccgacgg taacgggccc accaaaccga
      541 ccgacggtaa cgggcccacc aaaccgaccg acggtaacgg gcccaccaaa ccgaccgacg
      601 gtaacgggcc caccaaaccg accgacggta acgggcccac caaaccgacc gacggtaacg
      661 ggcccagaag agctaagtaa gtaagagctc gacccggtcg aatttgcttt cgaatttctg
      721 ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat
      781 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      841 taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg
      901 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      961 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1021 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1081 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1141 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1201 cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca
     1261 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1321 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1381 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1441 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1501 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     1561 cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat
     1621 ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg
     1681 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     1741 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     1801 agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
     1861 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
     1921 gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
     1981 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
     2041 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
     2101 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     2161 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
     2221 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     2281 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     2341 gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     2401 aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat
     2461 gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt
     2521 tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg
     2581 ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc
     2641 ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac
     2701 ctgaagtcag ccccatacga tataagttgt aattctcatg tttgacagca tgccatcgat
     2761 cgcgatcttg ctcaattgtt atcagctatg cgccgaccag aacaccttgc cgatcagcca
     2821 aacgtctctt caggccactg actagcgata actttcccca caacggaaca actctcattg
     2881 catgggatca ttgggtactg tgggtttagt ggttgtaaaa acacctgacc gctatccctg
     2941 atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg ctatgcagaa atcacctggc
     3001 tcaacagcct gctcagggtc aacgagaatt aacattccgt caggaaagct tggcttggag
     3061 cctgttggtg cggtcatgga attaccttca acctcaagcc agaatgcaga atcactggct
     3121 tttttggttg tgcttaccca tctctccgca tcacctttgg taaaggttct aagctcaggt
     3181 gagaacatcc ctgcctgaac atgagaaaaa acagggtact catactcact tctaagtgac
     3241 ggctgcatac taaccgcttc atacatctcg tagatttctc tggcgattga agggctaaat
     3301 tcttcaacgc taactttgag aatttttgca agcaatgcgg cgttataagc atttaatgca
     3361 ttgatgccat taaataaagc accaacgcct gactgcccca tccccatctt gtctgcgaca
     3421 gattcctggg ataagccaag ttcatttttc tttttttcat aaattgcttt aaggcgacgt
     3481 gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg tgctcatacg ttaaatc
//
