LOCUS       62056_3065        7047 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_150opt, complete sequence.
ACCESSION   MK606517
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(4310..4966)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4967..5069)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(5595..6140)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6327..7037)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccactaa accgactgat ggtaatggtc ctactaaacc tacggatggt aatggcccga
      181 cgaagccgac tgacggtaat ggccctacta aaccaactga tggcaacggt ccaactaagc
      241 ctaccgacgg taacggtcca acgaaaccga ctgacggcaa tggcccaact aaacctacgg
      301 acggcaatgg tccaaccaaa cctaccgatg gtaacggccc aaccaaaccg accgacggca
      361 acggtccgac caagccgacc gacggtaatg gtccgaccaa accaaccgat ggcaacggcc
      421 ctaccaagcc gactgatggc aatggtccga cgaaacctac tgacggtaac ggcccgacga
      481 aaccgacgga cggcaacggc cctactaaac cgactgatgg taatggtcct actaaaccta
      541 cggatggtaa tggcccgacg aagccgactg acggtaatgg ccctactaaa ccaactgatg
      601 gcaacggtcc aactaagcct accgacggta acggtccaac gaaaccgact gacggcaatg
      661 gcccaactaa acctacggac ggcaatggtc caaccaaacc taccgatggt aacggcccaa
      721 ccaaaccgac cgacggcaac ggtccgacca agccgaccga cggtaatggt ccgaccaaac
      781 caaccgatgg taatggtccc actaaaccga ctgatggtaa tggtcctact aaacctacgg
      841 atggtaatgg cccgacgaag ccgactgacg gtaatggccc tactaaacca actgatggca
      901 acggtccaac taagcctacc gacggtaacg gtccaacgaa accgactgac ggcaatggcc
      961 caactaaacc tacggacggc aatggtccaa ccaaacctac cgatggtaac ggcccaacca
     1021 aaccgaccga cggcaacggt ccgaccaagc cgaccgacgg taatggtccg accaaaccaa
     1081 ccgatggcaa cggccctacc aagccgactg atggcaatgg tccgacgaaa cctactgacg
     1141 gtaacggccc gacgaaaccg acggacggca acggccctac taaaccgact gatggtaatg
     1201 gtcctactaa acctacggat ggtaatggcc cgacgaagcc gactgacggt aatggcccta
     1261 ctaaaccaac tgatggcaac ggtccaacta agcctaccga cggtaacggt ccaacgaaac
     1321 cgactgacgg caatggccca actaaaccta cggacggcaa tggtccaacc aaacctaccg
     1381 atggtaacgg cccaaccaaa ccgaccgacg gcaacggtcc gaccaagccg accgacggta
     1441 atggtccgac caaaccaacc gatggtaatg gtcccactaa accgactgat ggtaatggtc
     1501 ctactaaacc tacggatggt aatggcccga cgaagccgac tgacggtaat ggccctacta
     1561 aaccaactga tggcaacggt ccaactaagc ctaccgacgg taacggtcca acgaaaccga
     1621 ctgacggcaa tggcccaact aaacctacgg acggcaatgg tccaaccaaa cctaccgatg
     1681 gtaacggccc aaccaaaccg accgacggca acggtccgac caagccgacc gacggtaatg
     1741 gtccgaccaa accaaccgat ggcaacggcc ctaccaagcc gactgatggc aatggtccga
     1801 cgaaacctac tgacggtaac ggcccgacga aaccgacgga cggcaacggc cctactaaac
     1861 cgactgatgg taatggtcct actaaaccta cggatggtaa tggcccgacg aagccgactg
     1921 acggtaatgg ccctactaaa ccaactgatg gcaacggtcc aactaagcct accgacggta
     1981 acggtccaac gaaaccgact gacggcaatg gcccaactaa acctacggac ggcaatggtc
     2041 caaccaaacc taccgatggt aacggcccaa ccaaaccgac cgacggcaac ggtccgacca
     2101 agccgaccga cggtaatggt ccgaccaaac caaccgatgg taatggtccc actaaaccga
     2161 ctgatggtaa tggtcctact aaacctacgg atggtaatgg cccgacgaag ccgactgacg
     2221 gtaatggccc tactaaacca actgatggca acggtccaac taagcctacc gacggtaacg
     2281 gtccaacgaa accgactgac ggcaatggcc caactaaacc tacggacggc aatggtccaa
     2341 ccaaacctac cgatggtaac ggcccaacca aaccgaccga cggcaacggt ccgaccaagc
     2401 cgaccgacgg taatggtccg accaaaccaa ccgatggcaa cggccctacc aagccgactg
     2461 atggcaatgg tccgacgaaa cctactgacg gtaacggccc gacgaaaccg acggacggca
     2521 acggccctac taaaccgact gatggtaatg gtcctactaa acctacggat ggtaatggcc
     2581 cgacgaagcc gactgacggt aatggcccta ctaaaccaac tgatggcaac ggtccaacta
     2641 agcctaccga cggtaacggt ccaacgaaac cgactgacgg caatggccca actaaaccta
     2701 cggacggcaa tggtccaacc aaacctaccg atggtaacgg cccaaccaaa ccgaccgacg
     2761 gcaacggtcc gaccaagccg accgacggta atggtccgac caaaccaacc gatggtaatg
     2821 gtcccactaa accgactgat ggtaatggtc ctactaaacc tacggatggt aatggcccga
     2881 cgaagccgac tgacggtaat ggccctacta aaccaactga tggcaacggt ccaactaagc
     2941 ctaccgacgg taacggtcca acgaaaccga ctgacggcaa tggcccaact aaacctacgg
     3001 acggcaatgg tccaaccaaa cctaccgatg gtaacggccc aaccaaaccg accgacggca
     3061 acggtccgac caagccgacc gacggtaatg gtccgaccaa accaaccgat ggcaacggcc
     3121 ctaccaagcc gactgatggc aatggtccga cgaaacctac tgacggtaac ggcccgacga
     3181 aaccgacgga cggcaacggc cctactaaac cgactgatgg taatggtcct actaaaccta
     3241 cggatggtaa tggcccgacg aagccgactg acggtaatgg ccctactaaa ccaactgatg
     3301 gcaacggtcc aactaagcct accgacggta acggtccaac gaaaccgact gacggcaatg
     3361 gcccaactaa acctacggac ggcaatggtc caaccaaacc taccgatggt aacggcccaa
     3421 ccaaaccgac cgacggcaac ggtccgacca agccgaccga cggtaatggt ccgaccaaac
     3481 caaccgatgg taatggtccc actaaaccga ctgatggtaa tggtcctact aaacctacgg
     3541 atggtaatgg cccgacgaag ccgactgacg gtaatggccc tactaaacca actgatggca
     3601 acggtccaac taagcctacc gacggtaacg gtccaacgaa accgactgac ggcaatggcc
     3661 caactaaacc tacggacggc aatggtccaa ccaaacctac cgatggtaac ggcccaacca
     3721 aaccgaccga cggcaacggt ccgaccaagc cgaccgacgg taatggtccg accaaaccaa
     3781 ccgatggcaa cggccctacc aagccgactg atggcaatgg tccgacgaaa cctactgacg
     3841 gtaacggccc gacgaaaccg acggacggca acggccctac taaaccgact gatggtaatg
     3901 gtcctactaa acctacggat ggtaatggcc cgacgaagcc gactgacggt aatggcccta
     3961 ctaaaccaac tgatggcaac ggtccaacta agcctaccga cggtaacggt ccaacgaaac
     4021 cgactgacgg caatggccca actaaaccta cggacggcaa tggtccaacc aaacctaccg
     4081 atggtaacgg cccaaccaaa ccgaccgacg gcaacggtcc gaccaagccg accgacggta
     4141 atggtccgac caaaccaacc gatggtaatg gtcccagaag agctaagtaa gtaagagctc
     4201 gacccggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag
     4261 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     4321 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     4381 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     4441 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     4501 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     4561 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     4621 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     4681 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     4741 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     4801 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     4861 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     4921 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     4981 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     5041 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg
     5101 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca
     5161 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta
     5221 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     5281 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt
     5341 gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag
     5401 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc
     5461 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga
     5521 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc
     5581 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     5641 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc
     5701 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     5761 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     5821 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta
     5881 tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa
     5941 ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa
     6001 gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga
     6061 gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc
     6121 gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt
     6181 cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt
     6241 aattctcatg tttgacagca tgccatcgat cgcgatcttg ctcaattgtt atcagctatg
     6301 cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata
     6361 actttcccca caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt
     6421 ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc
     6481 ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt
     6541 aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca
     6601 acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca
     6661 tcacctttgg taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa
     6721 acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg
     6781 tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca
     6841 agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct
     6901 gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc
     6961 tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt
     7021 ttcttttttg tgctcatacg ttaaatc
//
