LOCUS       62056_3060        3402 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_15, complete sequence.
ACCESSION   MK606511
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(665..1321)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1322..1424)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1950..2495)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2682..3392)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccaccaa accgaccgac ggtaacgggc ccaccaaacc gaccgacggt aacgggccca
      181 ccaaaccgac cgacggtaac gggcccacca aaccgaccga cggtaacggg cccaccaaac
      241 cgaccgacgg taacgggccc accaaaccga ccgacggtaa cgggcccacc aaaccgaccg
      301 acggtaacgg gcccaccaaa ccgaccgacg gtaacgggcc caccaaaccg accgacggta
      361 acgggcccac caaaccgacc gacggtaacg ggcccaccaa accgaccgac ggtaacgggc
      421 ccaccaaacc gaccgacggt aacgggccca ccaaaccgac cgacggtaac gggcccacca
      481 aaccgaccga cggtaacggg cccaccaaac cgaccgacgg taacgggccc agaagagcta
      541 agtaagtaag agctcgaccc ggtcgaattt gctttcgaat ttctgccatt catccgctta
      601 ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa
      661 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      721 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      781 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
      841 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
      901 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      961 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1021 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1081 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     1141 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1201 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1261 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1321 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1381 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     1441 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     1501 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     1561 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
     1621 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
     1681 cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag
     1741 tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag
     1801 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     1861 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     1921 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     1981 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     2041 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     2101 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     2161 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     2221 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     2281 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     2341 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     2401 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     2461 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     2521 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     2581 tacgatataa gttgtaattc tcatgtttga cagcatgcca tcgatcgcga tcttgctcaa
     2641 ttgttatcag ctatgcgccg accagaacac cttgccgatc agccaaacgt ctcttcaggc
     2701 cactgactag cgataacttt ccccacaacg gaacaactct cattgcatgg gatcattggg
     2761 tactgtgggt ttagtggttg taaaaacacc tgaccgctat ccctgatcag tttcttgaag
     2821 gtaaactcat cacccccaag tctggctatg cagaaatcac ctggctcaac agcctgctca
     2881 gggtcaacga gaattaacat tccgtcagga aagcttggct tggagcctgt tggtgcggtc
     2941 atggaattac cttcaacctc aagccagaat gcagaatcac tggctttttt ggttgtgctt
     3001 acccatctct ccgcatcacc tttggtaaag gttctaagct caggtgagaa catccctgcc
     3061 tgaacatgag aaaaaacagg gtactcatac tcacttctaa gtgacggctg catactaacc
     3121 gcttcataca tctcgtagat ttctctggcg attgaagggc taaattcttc aacgctaact
     3181 ttgagaattt ttgcaagcaa tgcggcgtta taagcattta atgcattgat gccattaaat
     3241 aaagcaccaa cgcctgactg ccccatcccc atcttgtctg cgacagattc ctgggataag
     3301 ccaagttcat ttttcttttt ttcataaatt gctttaaggc gacgtgcgtc ctcaagctgc
     3361 tcttgtgtta atggtttctt ttttgtgctc atacgttaaa tc
//
