LOCUS       62056_3055        3348 bp DNA     circular SYN 02-OCT-2019
DEFINITION  Cloning vector pAMP1_HisA_HBVep_13, complete sequence.
ACCESSION   MK606510
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Skowron PM, Zylicz-Stachula A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2019) University of Gdansk, Faculty of Chemistry, 
            Wita Stwosza 63, Gdansk, Pomorskie 80-308, Poland
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2019) University of Gdansk, Faculty of Chemistry, Wita 
            Stwosza 63, Gdansk, Pomorskie 80-308, Poland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             96..113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(611..1267)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1268..1370)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1896..2441)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2628..3338)
                     /codon_start=1
                     /label=lambda repressor
                     /note="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt
       61 gataatggtt gcatgtacta aggaggttgt tcatgcacca ccaccaccac cacagctctt
      121 cacccaccaa accgaccgac ggtaacgggc ccaccaaacc gaccgacggt aacgggccca
      181 ccaaaccgac cgacggtaac gggcccacca aaccgaccga cggtaacggg cccaccaaac
      241 cgaccgacgg taacgggccc accaaaccga ccgacggtaa cgggcccacc aaaccgaccg
      301 acggtaacgg gcccaccaaa ccgaccgacg gtaacgggcc caccaaaccg accgacggta
      361 acgggcccac caaaccgacc gacggtaacg ggcccaccaa accgaccgac ggtaacgggc
      421 ccaccaaacc gaccgacggt aacgggccca ccaaaccgac cgacggtaac gggcccagaa
      481 gagctaagta agtaagagct cgacccggtc gaatttgctt tcgaatttct gccattcatc
      541 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
      601 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      661 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
      721 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
      781 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
      841 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
      901 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
      961 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     1021 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     1081 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     1141 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1201 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     1261 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     1321 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     1381 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     1441 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     1501 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     1561 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     1621 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     1681 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     1741 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1801 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     1861 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     1921 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     1981 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     2041 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     2101 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     2161 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     2221 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     2281 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     2341 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     2401 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     2461 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
     2521 gccccatacg atataagttg taattctcat gtttgacagc atgccatcga tcgcgatctt
     2581 gctcaattgt tatcagctat gcgccgacca gaacaccttg ccgatcagcc aaacgtctct
     2641 tcaggccact gactagcgat aactttcccc acaacggaac aactctcatt gcatgggatc
     2701 attgggtact gtgggtttag tggttgtaaa aacacctgac cgctatccct gatcagtttc
     2761 ttgaaggtaa actcatcacc cccaagtctg gctatgcaga aatcacctgg ctcaacagcc
     2821 tgctcagggt caacgagaat taacattccg tcaggaaagc ttggcttgga gcctgttggt
     2881 gcggtcatgg aattaccttc aacctcaagc cagaatgcag aatcactggc ttttttggtt
     2941 gtgcttaccc atctctccgc atcacctttg gtaaaggttc taagctcagg tgagaacatc
     3001 cctgcctgaa catgagaaaa aacagggtac tcatactcac ttctaagtga cggctgcata
     3061 ctaaccgctt catacatctc gtagatttct ctggcgattg aagggctaaa ttcttcaacg
     3121 ctaactttga gaatttttgc aagcaatgcg gcgttataag catttaatgc attgatgcca
     3181 ttaaataaag caccaacgcc tgactgcccc atccccatct tgtctgcgac agattcctgg
     3241 gataagccaa gttcattttt ctttttttca taaattgctt taaggcgacg tgcgtcctca
     3301 agctgctctt gtgttaatgg tttctttttt gtgctcatac gttaaatc
//
