LOCUS       pAMP1.        4114 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of a cloned cDNA. For an 
            MCS with FseI and SalI sites, use pAMP10.
ACCESSION   .
VERSION     .
KEYWORDS    pAMP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..156
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        164..182
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    191..291
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(305..323)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(342..358)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(366..382)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(390..420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(435..456)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(472..1551)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1552..1629)
                     /label=lacI promoter
     rep_origin      complement(1907..2495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2669..3526)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3527..3631)
                     /label=AmpR promoter
     rep_origin      complement(3658..4113)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
       61 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      121 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
      181 gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccgcct
      241 actactacta atgatgatga tgcccgggaa ttccggaccg gtacctgcag gcgtaccagc
      301 tttccctata gtgagtcgta ttagagcttg gcgtaatcat ggtcatagct gtttcctgtg
      361 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      421 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      481 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      541 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      601 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      661 ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac atgagctgtc
      721 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
      781 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      841 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      901 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      961 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     1021 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     1081 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     1141 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacccg
     1201 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     1261 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     1321 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     1381 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1441 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1501 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1561 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1621 gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct ctgcattaat
     1681 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2101 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2341 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2581 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2641 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2701 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2761 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2821 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2881 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2941 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3001 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3061 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3541 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3601 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa
     3661 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3721 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3781 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3841 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3901 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3961 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4021 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4081 ccgccgcgct taatgcgccg ctacagggcg cgtc
//
