LOCUS       Exported                6245 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Exported.
ACCESSION   V009520
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6245)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6245)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(502..1233)
                     /gene="ermC"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             1698..1982
                     /codon_start=1
                     /note="unnamed protein product; copG-like"
                     /protein_id="SJL86728.1"
                     /translation="MSDFSERDRELLAMFGMGEDQVRDDVERMESETAGHGITGPVHYA
                     LHMLPEHGEEMVSMTVKLPKSQLEKVTETARRYHISRSEYVRRQLAGAV"
     CDS             complement(2174..2464)
                     /codon_start=1
                     /note="unnamed protein product; orfX-like"
                     /protein_id="SJL86729.1"
                     /translation="MTIQTIRKKRPLPAKELAAMYDVSVRTIQRWASQTRKDWIDEQAT
                     LRESIRAYHDDEGHTWPQTAEHFGMSQDAVRSRCYRARKERAAEAKAARPE"
     CDS             complement(2461..3408)
                     /codon_start=1
                     /note="unnamed protein product; repB"
                     /protein_id="SJL86730.1"
                     /translation="MSDEYSQPTLELSRTFEGWWLPRRPLCCDDDYSQLVRRSRTDALR
                     CKHIEANPSALVNTIVVDIDDANAKAMALWGHRGMLPNWIAENPANGHAHAGWVLTYPV
                     PRTDMARLKPLKLLHAVTEGLRRSVDGDEGYSGLLMKNPLSDAWDSDLCREDTYDLPDL
                     VAALEEHGDMPPKSWTRTKRAREVGVGRNCTLFDEARTLAYRQVRRLPDRTPASSDLLR
                     EYVRRTCHEINASFPDPLPVREVNDTAKSIHKWITTRSRMWRDGAVANAATFVAIQSAR
                     GRKGGRGNKRDKQGNVQNAFKQKAELFGKEMMGQ"
     primer_bind     complement(4024..4040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4048..4064)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4072..4102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4117..4138)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4426..5014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5188..6045)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6046..6150)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accccgggtc
      421 gacaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt aagggatgca
      481 gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa
      541 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa
      601 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt
      661 atacttttgt ttatctttgt gtgatattct tgattttttt ctacttaatc tgataagtga
      721 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat
      781 atcaacttct gccattaaaa gtaatgccaa tgagcgtttt gtatttaata atcttttagc
      841 aaacccgtat tccacgatta aataaatctc attagctata ctatcaaaaa caattttgcg
      901 tattatatcc gtacttatgt tataaggtat attaccatat attttatagg attggttttt
      961 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag
     1021 tttattttct gtagttttgc ataatttatg gtctatttca atggcagtta cgaaattaca
     1081 cctctttact aattcaaggg taaaatggcc ttttcctgag ccgatttcaa agatattatc
     1141 atgttcattt aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa
     1201 gttttgactg tgttttatat ttttctcgtt cattataacc ctctttaatt tggttatatg
     1261 aattttgctt attaacgatt cattataacc acttattttt tgtttggttg ataatgaact
     1321 gtgctgatta caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata
     1381 attatagcac gagctctgat aaatatgaac atgatgagtg atcgttaacc gggggggatc
     1441 tacgtgctgc cgcgcgtgca tgagcggcat cccgagctgt ccgtggagga cgtgcttgcg
     1501 gcgttcagga gcgtcatggt ggacgcccgc agggacaacg gcacgtgggt ctgcatcggc
     1561 ctcgacgggc gcggcaggga cgtggagatg gtctacgagc agcgcggcga ccgcgcgctc
     1621 atctaccacg ccatgacgcc gccgacgaag aagacccgaa gcgagatagc ccgcctgcgc
     1681 gggggaagga gcgaccgatg agcgatttca gcgaacgcga cagggagctg ttggcgatgt
     1741 tcggcatggg cgaggatcag gtcagggacg acgtggagcg catggagtcc gagaccgcgg
     1801 gccacggcat aaccggaccc gtccactacg cgctgcacat gctccccgag cacggcgagg
     1861 agatggtcag catgaccgtc aagctgccca agagtcagct ggagaaggtc acggagaccg
     1921 ccaggcgcta ccacatcagc cgcagcgagt acgtgcgccg gcagctcgcc ggagccgtct
     1981 gaccgccgcc gcgcgaagac ggaacacccg tgtgccatca ttcgtggcac acgggtgttc
     2041 ccataggtga caccgcggtg tcaccatgcc gcgcgagcgg tctggaggcg cagccgacca
     2101 tggggtacgg gtcttcccgg caagccgatt cgcggtgcga atcgagtgaa cgtatatacg
     2161 ttcacgtcac ttgctactcc ggccttgcgg cttttgcctc ggccgcacgc tccttgcgcg
     2221 cgcggtagca gcggctacgc accgcgtctt ggctcatgcc gaagtgctcg gcggtctgcg
     2281 gccacgtatg gccctcgtca tcgtggtagg cacggatgga ttcgcgcaac gttgcctgtt
     2341 cgtctatcca gtccttacgg gtttgtgaag cccatcgctg aatagtgcgc acggagacgt
     2401 cgtacattgc cgccagctcc ttggcgggaa gcggacgctt cttgcgaatc gtctgaatcg
     2461 tcattgcccc atcatctcct tgccgaacag ctctgccttt tgcttgaagg cattttgaac
     2521 gttcccttgc ttgtcgcgtt tgtttccgcg accgcctttg cgtccgcgcg cggattggat
     2581 ggcgacgaac gtggcggcgt tggcgacggc accgtccctc cacatgcggc ttcgcgtggt
     2641 gatccacttg tggatgctct tggcggtgtc gttgacctcg cgcacgggca gcgggtcggg
     2701 gaacgaggcg ttgatttcgt ggcaggtgcg gcgcacgtac tcgcgcagca ggtcggagga
     2761 ggcgggcgtg cggtcgggca gtcggcgcac ctgacggtag gcgagggtgc gggcctcgtc
     2821 gaacaaggtg cagttgcggc ccacgccgac ctcgcgggcg cgtttggtgc gcgtccagct
     2881 cttgggcggc atgtccccgt gctcttcgag cgcggccacg aggtcgggca ggtcgtaggt
     2941 gtcctcgcgg cacaggtcgc tgtcccacgc gtcgctcagc gggttcttca tcagcaggcc
     3001 ggaatagccc tcgtcgccgt ccacgctgcg gcgcagcccc tcggtgacgg cgtgcaggag
     3061 cttcagcggt ttgagccggg ccatgtcggt gcggggcacg gggtaggtca gcacccagcc
     3121 cgcgtgagcg tgcccgttgg ccgggttctc cgcgatccag ttcggcagca tcccacggtg
     3181 cccccacagg gccatcgcct tggcgttcgc gtcgtcgatg tccaccacga tcgtgttcac
     3241 cagcgccgag ggattcgcct cgatgtgctt gcatctgagc gcgtcggtgc ggctccggcg
     3301 caccagctgg ctgtagtcgt cgtcgcagca cagcggacgc cggggcagcc accagccctc
     3361 gaacgtgcgc gacagctcaa gcgtcggctg cgaatactcg tcagacatgg gcactagtgt
     3421 acgtcgtact tgaccactgg tcaagttcgc ctaaaaccaa gtcagatatt tcagcccagc
     3481 aaagcatggg ctgctccttc ccagagttac ccaaaactgt cgaaatctgg ccaaaaacac
     3541 cccaaaccga acgatcatcc accctcatgc gcttcctatg cgctcagatg gatatatcca
     3601 tggataccca catggacacg tctatggaca cccacatgga tatgtccatg gacatccaca
     3661 tggagatgtc catcaaatcg aaggtaagca agctagacgg ttcatgtaca tgaaccgaac
     3721 gcagcacctc ctgcgccctt cggcgcagga gcaacaaccc gaggccgagg aaaaagccgc
     3781 aaggccggag tagcaagcga cgtgattgta gctacaatca ctcgattcgc ggtgcgaatc
     3841 ggcttgccgg gaagacccgt accccatggt cggcggagcc tccagaccgc tcgcgcggct
     3901 tgtgccacaa cggtgttcca cttgtgtgac cgtggtgtta ctctggtatc aactttgctt
     3961 ggaggtgctg tttggagatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc
     4021 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagatc cacgctcacc
     5341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     6241 tcgtc
//
