LOCUS       62056_2965        6754 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        59..2394
                     /label=Alb
                     /note="Mouse albumin promoter"
     primer_bind     complement(2434..2450)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2496..3524
                     /codon_start=1
                     /label=Cre
                     /note="site-specific recombinase"
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    3542..3663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3670..4125)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4152..4256
                     /label=AmpR promoter
     promoter        4258..4615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4650..5441
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5676..5723
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6052..6640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctcc accgcggtgg cggccgctct
       61 agcttcctta gcatgacgtt ccactttttt ctaaggtgga gcttacttct ttgatttgat
      121 cttttgtgaa acttttggaa attacccatc ttcctaagct tctgcttctc tcagttttct
      181 gcttgctcat tccacttttc cagctgaccc tgccccctac caacattgct ccacaagcac
      241 aaattcatcc agagaaaata aattctaagt tttatagttg tttggatcgc ataggtagct
      301 aaagaggtgg caacccacac atccttaggc atgagcttga ttttttttga tttagaacct
      361 tcccctctct gttcctagac tacactacac attctgcaag catagcacag agcaatgttc
      421 tactttaatt actttcattt tcttgtatcc tcacagccta gaaaataacc tgcgttacag
      481 catccactca gtatcccttg agcatgaggt gacactactt aacataggga cgagatggta
      541 ctttgtgtct cctgctctgt cagcagggca ctgtacttgc tgataccagg gaatgtttgt
      601 tcttaaatac catcattccg gacgtgtttg ccttggccag ttttccatgt acatgcagaa
      661 agaagtttgg actgatcaat acagtcctct gcctttaaag caataggaaa aggccaactt
      721 gtctacgttt agtatgtggc tgtagaaagg gtatagatat aaaaattaaa actaatgaaa
      781 tggcagtctt acacattttt ggcagcttat ttaaagtctt ggtgttaagt acgctggagc
      841 tgtcacagct accaatcagg catgtctggg aatgagtaca cggggaccat aagttactga
      901 cattcgtttc ccattccatt tgaatacaca cttttgtcat ggtattgctt gctgaaattg
      961 ttttgcaaaa aaaacccctt caaattcata tatattattt taataaatga attttaattt
     1021 atctcaatgt tataaaaaag tcaattttaa taattaggta cttatatacc caataatatc
     1081 taacaatcat ttttaaacat ttgtttattg agcttattat ggatgaatct atctctatat
     1141 actctatata ctctaaaaaa gaagaaagac catagacaat catctatttg atatgtgtaa
     1201 agtttacatg tgagtagaca tcagatgctc catttctcac tgtaatacca tttatagtta
     1261 cttgcaaaac taactggaat tctaggactt aaatatttta agttttagct gggtgactgg
     1321 ttggaaaatt ttaggtaagt actgaaacca agagattata aaacaataaa ttctaaagtt
     1381 ttagaagtga tcataatcaa atattaccct ctaatgaaaa tattccaaag ttgagctaca
     1441 gaaatttcaa cataagataa ttttagctgt aacaatgtaa tttgttgtct attttctttt
     1501 gagatacagt tttttctgtc tagctttggc tgtcctggac cttgctctgt agaccaggtt
     1561 ggtcttgaac tcagagatct gcttgcctct gccttgcaag tgctaggatt aaaagcatgt
     1621 gccaccactg cctggctaca atctatgttt tataagagat tataaagctc tggctttgtg
     1681 acattaatct ttcagataat aagtcttttg gattgtgtct ggagaacata cagactgtga
     1741 gcagatgttc agaggtatat ttgcttaggg gtgaattcaa tctgcagcaa taattatgag
     1801 cagaattact gacacttcca ttttatacat tctacttgct gatctatgaa acatagataa
     1861 gcatgcaggc attcatcata gttttcttta tctggaaaaa cattaaatat gaaagaagca
     1921 ctttattaat acagtttaga tgtgttttgc catcttttaa tttcttaaga aatactaagc
     1981 tgatgcagag tgaagagtgt gtgaaaagca gtggtgcagc ttggcttgaa ctcgttctcc
     2041 agcttgggat cgacctgcag gcatgcttcc atgccaaggc ccacactgaa atgctcaaat
     2101 gggagacaaa gagattaagc tcttatgtaa aatttgctgt tttacataac tttaatgaat
     2161 ggacaaagtc ttgtgcatgg gggtgggggt ggggttagag gggaacagct ccagatggca
     2221 aacatacgca agggatttag tcaaacaact ttttggcaaa gatggtatga ttttgtaatg
     2281 gggtaggaac caatgaaatg cgaggtaagt atggttaatg atctacagtt attggttaaa
     2341 gaagtatatt agagcgagtc tttctgcaca cagatcacct ttcctatcaa ccccgggatc
     2401 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
     2461 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggc caatttactg accgtacacc
     2521 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
     2581 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
     2641 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
     2701 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
     2761 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
     2821 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
     2881 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
     2941 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
     3001 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
     3061 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
     3121 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
     3181 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
     3241 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
     3301 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
     3361 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
     3421 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
     3481 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattagcaa ttgttgttgt
     3541 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3661 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     3721 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3781 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     3841 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     3901 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     3961 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4021 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4081 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     4141 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4201 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     4261 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4321 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4381 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4441 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4501 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4561 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     4621 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4681 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4741 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4801 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     4861 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4921 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4981 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5041 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5101 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5161 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5221 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5281 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5341 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5401 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5461 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5521 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5581 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     5641 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     5701 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     5761 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     5821 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     5881 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     5941 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6001 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6061 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6181 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6721 tgattctgtg gataaccgta ttaccgccat gcat
//
