LOCUS       62056_2960        4082 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      20..47
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     rep_origin      complement(537..1125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1412..2203)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 actagttgat tagctaagca gaaggccatc ctgacggatg gcctttttgc gtttaatact
       61 gtttaaactc tagaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat
      121 ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc
      181 ggcgttgatc tgtgcgttcg ctgtgcgggc tctaacacgt cctagtatgg taggatgagc
      241 aacatttcga cgccgagaga ttcgccgccc gaaatgagca cgatccgcat gcttaattaa
      301 gaaggagata tacattagtg gatcccccgg gctgcaggaa ttcgatatca agcttatcga
      361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      721 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      961 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1201 agattatcaa aaaggatctt cacctagatc cttttggggt gggcgaagaa ctccagcatg
     1261 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     1321 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     1381 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     1441 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     1501 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     1561 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     1621 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atccgcgcct
     1681 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     1741 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     1801 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     1861 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     1921 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     1981 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac
     2041 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     2101 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     2161 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     2221 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     2281 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     2341 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag
     2401 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat
     2461 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc
     2521 agcccttgcg ccctgagtgc ttgcggcagc gtgaagctag cgtctaccag gacttttacc
     2581 tgtccgaccc gttgcaacgg aaccccccac ggaacccccg cgacacccgc tccccaattg
     2641 cgttagaaca gcggtggatt gtcggcttcg ttgtgggcct tttgagccgc ttcctgttct
     2701 gccgcacgct ctttcctcgc ccgatagccg agtcgcttaa cggtgtccag atgcagcccg
     2761 aaatgtttgg ccgtttgcgg ccaagagtgg ccctcgtcgt cgtgataggc gcggatgcgt
     2821 tcgcggcgtg cagcctgctc ggcgagccac tcgctgcgtt cctgcgccac gagccggacg
     2881 acgtggcgtt cggatagtcc ggtgattcga gcgccttcgg cggcggtcac gcgccgcttt
     2941 ttgcggacag tcggctgccg gttgtagccg tcgctgtagc cgtcgctcat agcaatgcct
     3001 ccatggctga cgcggacttt gcgcgccgcg caactgtgct cgccgccgtg cgcgctgctg
     3061 cgcccttccg cgagatggcc gactggcgcg cactgagtgt ggcctcgtag accacgatcc
     3121 cgtccgccca aatgcgcgac ttggttgtga tccaacgcca aatgctgttg gcgatggcgc
     3181 ggacctcgct gtccggtagc ggtccgggac acacgtcgtt gcacgggaac tcggcgtttc
     3241 gcgcgtggca ctcggcatag atcgcgcggc cgagtccgtc cacgttccgg gtcggcaggt
     3301 agatccgcat gagggcggga cgataggccc acaacctgac ggaatcgaac agtgcgcaat
     3361 tccgccctag cggcgtcgga gccgctttgt acgtggtctg ctgacgccag cgcggcggtg
     3421 gcatgttcgc gccgagctcg gcctcgatgt ggctgagtgt gtagagatct gagtggagcc
     3481 attccgtttc ccaggcgatg tggccggggt ttttggtcat gaggcctgag taactgcggt
     3541 cgccgtccac ggcgcgccga aggccttcgg cgcacgccgc catgtatgcg agcggcttac
     3601 gccgcgcgta ttcggtgcgt ggaacagggg cgttgagtgc ccacactgcg tgtgcgtggc
     3661 cgttggcgcg attgcccacg atcgcgttgg gcagcggatg ggacccccgg gcgctgagcg
     3721 ctcggagcgc tgcgtctgga tggtctacgt ccacgaccag caggtttgcc agcgctgttg
     3781 ggttcgcctc gatgtaccgg cggcctaggg ccgacgcgcg gctttggcgg tagatcccct
     3841 cgagcagatc gtcgcttgcc agcggccagt acggcagcca gagctgctca aattcgtcgg
     3901 cgacgtggct cacgcttggt agtagaccac gattaatcac cggtgtatgg tccgacacga
     3961 gctccaagtc agatatttcg ctgaggggcc accccacaac tgcacactcc cccgctctcc
     4021 cgtcgagccc tggtggtgga acaccagcga cagccgagca cccccaacca cctgtaccaa
     4081 cc
//
