LOCUS       40924_4329       11418 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pAL36, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Karim AA, Gestaut DR, Fincker M, Ruth JC, Holmes EC, Sheu W, 
            Spormann AM.
  TITLE     Fine-tuned Protein Production in Methanosarcina acetivorans C2A
  JOURNAL   ACS Synth Biol (2018) In press
  PUBMED    29920209
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Karim AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2017) Civil and Environmental Engineering, 
            Stanford University, Y2E2, 473 Via Ortega, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 11418)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11418)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-NOV-2017) Civil and Environmental Engineering, Stanford 
            University, Y2E2, 473 Via Ortega, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..90
                     /label=minimal mcrB promoter with tetracycline operator
                     /note="minimal mcrB promoter with tetracycline operator"
                     /regulatory_class="promoter"
     protein_bind    46..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      91..109
                     /label=mcrB RBS from Methanosarcina acetivorans C2A
                     /note="mcrB RBS from Methanosarcina acetivorans C2A"
                     /regulatory_class="ribosome_binding_site"
     CDS             110..3181
                     /label=lacZ
                     /note="beta-galactosidase"
     misc_feature    3192..8662
                     /label=pC2A sequence
                     /note="pC2A sequence"
     CDS             complement(3798..4742)
                     /codon_start=1
                     /gene="ssrA"
                     /product="site-specific recombinase A"
                     /label=ssrA
                     /protein_id="AWW92034.1"
                     /translation="MGEMAAIQLTGIYEPLDNERLISLFTSDCIGRGYSKRTIEGYVSH
                     AKFFLQMCGVNAGFPELEGFLVHIRDERQYTLATCNNYFASLSTFFDFLEFKHIIERNN
                     IPTFRKRYLRSYKKYHTPETRQLITIEDMAKLVKVPRYPMYQALIIFLAKTGIRRNELI
                     TLDRADIDLEKCTVRLKKTAKRSNRIVFFDLETKAFLEAYLNTRHDKEKALFIGRQNGH
                     RVQRNLVYDIVTSAASSIGLHNPEGRLEEHFTPHCCRHWFTTWLRRSGMERTFIQELRG
                     DSRGEAIDVYDHIEKDELREAYLKCIPQLGIKP"
     gene            complement(3798..4742)
                     /gene="ssrA"
                     /label=ssrA
     misc_feature    5225..5872
                     /label=Orf2
                     /note="Orf2"
     misc_feature    complement(5878..6306)
                     /label=Orf1
                     /note="Orf1"
     CDS             complement(6385..7995)
                     /codon_start=1
                     /gene="repA"
                     /product="regulatory protein RepA"
                     /label=repA
                     /protein_id="AWW92035.1"
                     /translation="MSSDFSSRPGAVSYAPECRDFATCICTDGVLCPVRNKKNGVSICK
                     YYSCPDFDFSKCSKYRPLKVGDFVLPAKGKRPAYCGRPFTYAVSENYNARKDLNSHCNT
                     LSCPGCGILSELQHRFALVVILWTYALFSGQFPSWGFASMDFSRGVSVEAIRLFRRNLK
                     DRLERWGVTAGTTIFHPYRIKPEITRAIRKLTGLKGNDDSEAFWNYIRDDHNEGNLNKI
                     ADLLNIEINSIYDCLILAPHFHFFIFPGNIRITGKNPKSKKRCKSDEYDIFIQRLSHKV
                     NGKRQWELRSLMDIYKQVTYLYSHVGQLTDNRYGNIHIESRFGALFRWHPDKVVSSSGK
                     LTFEQLIDTRTEVLNLINEGREVKLVYNGELKWEGQENDDTGWIPLTKLRDHFSGGRQA
                     LHAYLKEAKAVKPEYADYLQQLIARNNFYHNLKGRDGKNKVPKKWRKLWASPFKVDELP
                     EFLKLEPAGSLLRVWFLEGLPDPPAGFLVVDSHQWEHDGCIIEESPEMPSNSLQKASEI
                     NYEELFTSLKSRSMMFGGD"
     gene            complement(6385..7995)
                     /gene="repA"
                     /label=repA
     regulatory      8659..9020
                     /label=mcr promoter from Methanococcus voltae
                     /note="mcr promoter from Methanococcus voltae"
                     /regulatory_class="promoter"
     CDS             9021..9617
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     regulatory      9621..9960
                     /label=mcr terminator from Methanococcus voltae
                     /note="mcr terminator from Methanococcus voltae"
                     /regulatory_class="terminator"
     rep_origin      complement(9978..10366)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      complement(10395..10427)
                     /label=bla terminator
                     /note="bla terminator"
                     /regulatory_class="terminator"
     CDS             complement(10431..11288)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11289..11393)
                     /label=AmpR promoter
ORIGIN
        1 ggatccgcat gcttcattta tcggagaaca caaaagattt aagtacccta tcagtgatag
       61 agatttcatt gggaatagtg gacactcgag aattaaggag gaaattaaaa tgaccatgat
      121 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      181 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      241 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
      301 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
      361 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
      421 gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
      481 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
      541 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca
      601 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg
      661 cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg
      721 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag
      781 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga
      841 agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg
      901 tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg
      961 tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc
     1021 cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat
     1081 tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct
     1141 gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca
     1201 tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa
     1261 ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga
     1321 ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat
     1381 gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat
     1441 ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg
     1501 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc
     1561 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta
     1621 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg
     1681 gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg
     1741 taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca
     1801 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa
     1861 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat
     1921 gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca
     1981 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct
     2041 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct
     2101 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc
     2161 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc
     2221 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc
     2281 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag
     2341 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg
     2401 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca
     2461 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc
     2521 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt
     2581 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca
     2641 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca
     2701 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg
     2761 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca
     2821 ggaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc
     2881 agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga
     2941 attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag
     3001 tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg
     3061 gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt
     3121 atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa
     3181 ataaggtacc tctagtaagt tatagaaatg tcatccgata ccgaaaaaga aaacggaata
     3241 aatcagtaac aaatcaaaaa aacaaaacta taacatttaa tcttcgtaaa atcaatatca
     3301 atcgaccata cacaaaacat gttcaaataa aatacatggg tgtacatatg tcaaccgaca
     3361 ttgaaaaaga aaacggaata aatcagtaac aaatcaaaaa aacaaaacca taacatttct
     3421 ataacattcc atagagtaaa aatcaacatc gaataacctt acacaaacca tacataaacc
     3481 atacataaaa atacacatat gtcaaccgtt accgtaaaaa gattacttaa ataattttat
     3541 actcacctct tattttttgt gttttttatt atccagggca acctttccca gggagaaatt
     3601 cttccttgca gttgcaggca agaaccagga ctttcttcca gggcatactt tcccagggag
     3661 aaattcttcc ttgcagctgc aggcaagaac caggactttc ccccaggacc aggacctcaa
     3721 ggtctgcatc cagaggaaga ccttcccagg gcgccaagat cccgcagatt atggaacatt
     3781 atttcccggg aaataattca gggcttaatg cccaactgag gaatacattt taaataagct
     3841 tctcttagtt catccttttc tatgtggtcg tacacgtcta ttgcttcgcc tctcgaatct
     3901 ccccttaatt cctgaatgaa tgtcctttcc atacctgaac gtcgaagcca tgtagtgaac
     3961 caatgccggc agcaatgcgg agtaaaatgt tcttcaagtc tcccctcggg gttatggagt
     4021 ccgatagagg aagcggcaga tgtgacaata tcgtacacta aatttctttg tacacggtga
     4081 ccgttttgtc tgcctatgaa taatgctttt tctttatcat gcctcgtgtt aaggtacgct
     4141 tccaggaagg ccttagtctc aaggtcaaag aatacgatac gatttgacct tttagctgtc
     4201 ttcttgagtc tcaccgtaca cttttccaga tctatgtcag ctcgatcaag agtaattaat
     4261 tcatttctcc tgatgccagt tttagctaaa aagattatca atgcttgata catggggtaa
     4321 cgtggtactt tgacaagctt tgccatgtct tcaatggtaa tcaattgcct tgtttccgga
     4381 gtatggtatt ttttgtagct tcttagatag cgttttctga acgtgggaat gttgtttctc
     4441 tcgattatat gcttgaattc aaggaagtca aaaaaggtac tcagtgaagc aaagtaattg
     4501 ttacaggttg cgagtgtgta ctgcctttca tctcttatgt gaacaaggaa cccttcaagc
     4561 tccggaaacc ctgcattcac tccacacatt tgaaggaaaa acttagcatg acttacgtat
     4621 ccttctattg ttcttttaga gtaacccctt ccgatacaat cagaagtgaa taaacttata
     4681 agtctttcat tatcaagcgg ttcatagata ccagttagct gaatagctgc catttcaccc
     4741 ataaatcaat aaaagaagta tgtaaataaa tactttgtgc atagatgtca tttaaacaca
     4801 aagtataagt acatctacct tcttttagta tatgctggta acagcaaaaa agcgccaaac
     4861 aacgcatctt tacccaatag aatgacgtcg attaaaagag taagttggtg gtgagcctta
     4921 aaaaaggtaa aagagagctt actcttaagt taaaaacgga attggcaaac agtaaggatt
     4981 gttccagcag tccttactaa aaacattact ttattaattc tatatagtca caacattcac
     5041 aaaaataggc ttttttctcg atttttcctt ttgaatgttt ataaagaaaa atcatatagc
     5101 atttattaca ctgtggacat attttctttt caattttgcc ccttccaatt tgtggaagcc
     5161 ttttttcagt aacacgctta ggcataatga aagatcggtg tattaagtat taacattttc
     5221 taacttgtgc aataataatt attcggggtt gtcgaagaac ttccaaacag tagaccgatc
     5281 cattttccac attattctaa tgaagccaag actcaaggat ggggggaaat atccccccac
     5341 accccccaat gagactaccc aagacagccc gtatgccgac agcgacacag ttaaatatca
     5401 atgtgtatta atacatatca acaagtatca acacacacaa ttaaacaata atacacatca
     5461 atggagaaca atacacatgc caacacaaga atacacacgg acaaacatta cattaccaga
     5521 acatgttaga gcaatgatta aagagtacaa taaaagaagt acatggggag acctcaacgt
     5581 atcagcagta tgctctaatg caatagcaga cagaattaga gctacattcc caaaattaga
     5641 aaaagaacta tttccagaac aatttccaga acttaaccca aaaatgacaa aaaccagctc
     5701 accaggacaa ccagaaataa cacaaaaagg aaaatccaaa caaggagact ctaaagatag
     5761 aatatgcgct tattgtggcc aaccttttga accgaaatcg cataatcaaa aattctgtaa
     5821 agatgcatgt aagtcagcaa attatagaaa aaacaaaaaa acacagagtt aaacccatta
     5881 accacactct ttttttgaat ttatctgcat ttcacggtac tttttatcca agaaagccca
     5941 caaatcacgc tctttaaacg gtttcatgga aaaattttcc cggaatgcat taattaacat
     6001 cttttcaatt ttagggttga aataagcccc ataacggtaa tctggcgacc aatctgaagc
     6061 tttaacctct tcataagtcc gatacggcac attacccgga agaagcccag gaaccacaag
     6121 cagataatag atatagcacc ttatacacat atgtgatttt ttagtagtct ttaaccattc
     6181 aatatatgaa agatccgcac ctgttcgata atcctcaaaa agaattaagt tcctttgcct
     6241 ccgagatcca cacacacagc aatacttcaa ttccataata cataacaccc cgaaataata
     6301 agacatctga aaaaccgtat cactttaggc tttaagcatc ttatccggac cttcgagccc
     6361 aaaagccttg ttgaacgtgg catctcaatc acccccaaac atcatggagc gtgattttaa
     6421 gcttgtaaat agctcttcat aattaatttc ggaagccttt tgcaagctgt tagaaggcat
     6481 ttcaggcgat tcttcaatta tacagccatc atgctcccat tgatgagagt caaccaccag
     6541 gaagccggca gggggatcag gaagcccttc taagaaccat acccgaagaa gactcccggc
     6601 aggttcaagc ttcaaaaact ccggtaattc atccactttg aagggagaag cccataattt
     6661 acgccatttt ttaggaactt tgtttttgcc atctcgccct tttaggttat gataaaaatt
     6721 atttcttgca attaattgtt gcaagtaatc ggcatattca ggtttaaccg ctttagcttc
     6781 cttcaaataa gcatgtagag cctgtcttcc acccgaaaaa tgatcccgaa gctttgttaa
     6841 cggtatccat ccagtatcat cgttttcctg tccttcccac ttcaactctc cattataaac
     6901 gagttttact tctcgacctt cattgattaa attaaggact tcggttctgg tatctatcaa
     6961 ttgctcaaat gtcagctttc cggaggaact tacaacttta tcaggatgcc accggaacaa
     7021 agcaccaaag cgagattcta tatggatatt accgtatctg ttatccgtga gctgcccaac
     7081 gtgagaataa agataagtca cctgcttgta aatatccata aggctccgca attcccattg
     7141 ccttttacca ttgactttat gagaaagcct ctgaataaat atatcatatt catcactctt
     7201 gcatctcttt ttactcttcg gattctttcc agttatacga atgtttccag ggaaaatgaa
     7261 aaaatgaaaa tgaggagcaa ggataaggca gtcataaata gagtttattt cgatatttag
     7321 caagtccgcg atcttgttta aattgccttc gttgtggtcg tctcttatgt aattccaaaa
     7381 agcttcggag tcgtcattac cttttaaacc tgttaactta cggatagccc gagttatttc
     7441 cggcttaatc ctgtaagggt gaaagattgt agtaccagca gttacgcccc agcgttccag
     7501 cctgtccttt aaattcctcc ggaaaagtcg gattgcttcc acagatactc cccggctaaa
     7561 gtccatcgaa gcaaaccccc atgaaggaaa ctgcccagaa aacagggcat aagtccacaa
     7621 aattacaact aatgcaaatc tatgttgcaa ttccgatagg atgccgcaac caggacaaga
     7681 aagtgtattg cagtgagaat taagatcctt tcgtgcatta taattttcag atacagcata
     7741 agtgaaagga cgcccacagt aagcgggtct ttttcccttt gctggaagaa caaagtcgcc
     7801 aacttttaga ggacgatact tagagcattt agaaaaatcg aagtcaggac aagagtagta
     7861 tttacagata gaaaccccgt tctttttgtt gcgaaccggg cacaaaacac catcggtaca
     7921 tatacacgtc gcaaaatctc tacactctgg agcataggaa acggcgccag gtctagaact
     7981 aaaatcagaa ctcagagaac atttcctgtc gaaaatttaa accgtaaacc aaagataaca
     8041 attttcataa tattacagaa cctatatttt ttgtaactcg acattacaaa aatgtaccac
     8101 aaaacataaa aacattttca tagaagaaat attcacaaac aatagaaaaa aattataaga
     8161 ataagattaa cgcctacctt attaaaagaa ggtgtcgagt cttcaggttc catcaaggca
     8221 ggcgttgata ttctatatat gttacacata acatatataa catacgtacc tcagaacatt
     8281 cagacctgaa tgctctaaaa tgaaataagc tttaaacttt ataaattcat ctgctataaa
     8341 tagttttaga gatatagaaa gcccaaggaa gaaaaagaat agcaaaaata aaaaagaaaa
     8401 acggcccgcg acggcaaacc cggagactta taatcaaagt aagacgaaaa ggaagccgac
     8461 aacgatttga taataacaaa tcatctttta ttttggcaat attcatatga tatatgaaga
     8521 aatcaacatc aagcggatta gtatttttgc tatttgtaaa tcaatatcgg gtgagacagt
     8581 atatcaaaaa caaacttcat ttttttcgaa caaaaaaatc actatcaaat gacattgtag
     8641 taagaaacta catcaaaact agaggatgat taattttaag agagtcttaa aaaatcttcg
     8701 gaaaatagta gtaggtaata aaaaacgccc tattcgtaat atactattca aaactaaata
     8761 ttcattgaaa tatttgggta ataattaata ttaatctaat atttagatat tgagatataa
     8821 taataacatt tttttaaaaa atcatctatt ttcatcttaa ttttatatat taaagcccat
     8881 tttttgagta ttcaaattca aattattgtg ttattaacat cttatatata aacttttcta
     8941 tttaatgtta atgaaaaagt gaatatatat acatagagta atgttatgat gtatatatca
     9001 aaaaaatagg agtgattctc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9061 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     9121 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     9181 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     9241 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     9301 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     9361 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     9421 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     9481 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     9541 tcggcttcac cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga
     9601 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     9661 cacgacccca tggctccgac cgaagccacc cggggcggcc ccgccgaccc cgcacccgcc
     9721 cccgaggccc accgcggggg acacaccgaa cacgccgacc ctgctgaaca cgcggcgcag
     9781 ttcggtgccc aggagcggat cgggaattaa ttcgaagctg ctggtgaaag agaccctatc
     9841 ttacctgcta aaatctaagt taattactaa tttattatta atttattatt agattgggca
     9901 aaatagtaaa agaaaactaa aggaaaccta atatggtttc ctttttttat atatttttaa
     9961 ttcactgggg gcaattctgt cagccgttaa gtgttcctgt gtcactgaaa attgctttga
    10021 gaggctctaa gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac
    10081 cttaaaagct ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga
    10141 ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt
    10201 gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta
    10261 gttcgttaaa catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag
    10321 agcttagtac gtactatcaa caggttgaac tgctgatctt cagatcctct acgccggacg
    10381 catcgtgggg atctaaaaaa aagcccgctc attaggcggg ctgacagtta ccaatgctta
    10441 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10501 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10561 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10621 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10681 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10741 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10801 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10861 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10921 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10981 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    11041 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    11101 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    11161 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    11221 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    11281 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11341 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11401 ccccgaaaag tgccacct
//
