LOCUS       V009536                 3937 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009536
VERSION     V009536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A.
  TITLE     Markerless gene deletion system for sphingomonads
  JOURNAL   Appl. Environ. Microbiol. 78 (10), 3774-3777 (2012)
   PUBMED   22427496
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Department of Biology, Institute of
            Microbiology, ETH Zurich, Wolfgang-Pauli-Str. 10, Zurich, Zurich
            8093, Switzerland
REFERENCE   3  (bases 1 to 3937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "10";
            pages: "3774-3777"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2012) Department of Biology, Institute of Microbiology, ETH
            Zurich, Wolfgang-Pauli-Str. 10, Zurich, Zurich 8093, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             346..1137
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1167..1207
                     /gene="Psyn2"
                     /label="synthetic bacterial promoter"
                     /note="synthetic bacterial promoter"
                     /regulatory_class="promoter"
     gene            1167..1207
                     /gene="Psyn2"
                     /label="Psyn2"
     regulatory      1221..1229
                     /gene="rpsL1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1234..1602
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from
                     Rhizorhabdus wittichii (strain DSM 6014 / CCUG 31198 / JCM
                     15750 / NBRC 105917 / EY 4224 / RW1). Accession#: A5V607"
     oriT            complement(1625..1734)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        complement(1969..2059)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(2253..2343)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2703..3291
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3579..3600
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3615..3645
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3653..3669
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3677..3693
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    3702..3758
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3762..3778)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
       61 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
      121 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
      181 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
      241 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
      301 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      361 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      421 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      481 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
      541 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      601 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      661 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      721 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      781 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      841 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
      901 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      961 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     1021 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1081 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1141 gcgggactct ggggttcgct agggcccatg gttgacggat cgccgcgaag ccgctaactg
     1201 cgccggcaga tccatatacg aggaggaggc ttcatgccga ccatcaacca gctggtccgg
     1261 aaggggcgcg acccgcagaa ggcgaagtcg aaggtcccgg cgatggaaca gaacccccag
     1321 aagcgcggcg tgtgcacccg cgtctatacc accaccccga agaagcccaa ctcggcgctg
     1381 cgcaaggtcg cgaaggtccg cctgaccaac agccgcgagg tcatctcgta catccccggc
     1441 gagggccaca acctgcagga gcactcggtc gtcctgatcc gcggcggccg ggtcaaggac
     1501 ctgccgggcg tccgctatca cgtcctgcgc ggcgtgctgg acacccaggg cgtgaaggac
     1561 cgccgccagt cgcgctcgaa gtatggcgcg aagcgcccga agtgataacg cgtaccggcg
     1621 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     1681 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     1741 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     1801 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     1861 gcggaaaagc gctgcttccc tgctgttttg tggaatatct accgactgga aacaggcaaa
     1921 tgcaggaaat tactgaactg aggggacagg cgagagacga tgccaaagag ctcctgaaaa
     1981 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2041 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
     2101 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
     2161 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
     2221 tttgaggtgc tccagtggct tctgtttcta tcagctcctg aaaatctcga taactcaaaa
     2281 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     2341 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     2401 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     2461 tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     2521 ggcttctgtt tctatcaggg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     2581 ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3601 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3661 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgag ctcggtaccc
     3721 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
     3781 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3841 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3901 agcctgaatg gcgaatggcg ataagctagc ttcacgc
//
