LOCUS       V009537                 4091 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009537
VERSION     V009537
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Luo D, Wen C, Zhao R, Liu X, Liu X, Cui J, Liang JG, Liang P.
  TITLE     High Level Expression and Purification of Recombinant Proteins from
            Escherichia coli with AK-TAG
  JOURNAL   PLoS ONE 11 (5), E0156106 (2016)
   PUBMED   27214237
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Luo D.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4091)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4091)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2016"; volume: "11"; issue: "5"; pages: "E0156106"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2016) Department of Biochemistry "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             127..144
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             151..792
                     /gene="adk"
                     /label="Adenylate kinase"
                     /note="Adenylate kinase from Escherichia coli (strain K12 /
                     DH10B). Accession#: B1XFR1"
     misc_feature    793..822
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     terminator      838..932
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             976..1632
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     terminator      1700..1786
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1941..2081
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2267..2855)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3029..3886)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3887..3991)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatctcacc atcaccatca ccatgggatc ctgcgtatca ttctgcttgg cgctccgggc
      181 gcggggaaag ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc
      241 actggcgata tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa
      301 gacattatgg atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag
      361 cgcattgctc aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt
      421 ccgcaggcag acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac
      481 gtaccggacg aactgatcgt tgaccgtatc gtcggtcgcc gcgttcatgc gccgtctggt
      541 cgtgtttatc acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt
      601 gaagaactga ctacccgtaa agatgatcag gaagagaccg tacgtaaacg tctggttgaa
      661 taccatcaga tgacagcacc gctgatcggc tactactcca aagaagcaga agcgggtaat
      721 accaaatacg cgaaagttga cggcaccaag ccggttgctg aagttcgcgc tgatctggaa
      781 aaaatcctcg gcggtacccc gggtcgacct gcagccaagc ttaattagct gagcttggac
      841 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
      901 gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag attttcagga
      961 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1021 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1081 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1141 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg
     1201 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1261 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1321 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1381 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1441 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1501 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     1561 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1621 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     1681 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1741 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cctctagagc
     1801 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     1861 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1921 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     1981 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     2041 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     2101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4021 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4081 ttcgtcttca c
//
