LOCUS       40924_4259        3063 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAJM.944, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule 
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of 
            Biological Engineering, Massachusetts Institute of Technology, 77 
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3063)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg 
            285866, 1-26 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-MAR-2018) Synthetic Biology Center, Department of Biological 
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label=L3S3P21
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..298
                     /label=PCin
                     /note="PCin"
                     /regulatory_class="promoter"
     misc_feature    225..245
                     /label=CinO operator
                     /note="CinO operator"
     regulatory      246..251
                     /regulatory_class="minus_35_signal"
     regulatory      269..274
                     /regulatory_class="minus_10_signal"
     regulatory      281
                     /label=+1
                     /note="+1"
                     /regulatory_class="other"
     misc_RNA        300..350
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      382..393
                     /label=B0064
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             400..1116
                     /label=EYFP
                     /note="enhanced YFP"
     regulatory      1120..1180
                     /label=L3S2P21
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     rep_origin      complement(1514..2059)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2151..2963)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgccctttgt gcgtccaaac ggacgcacgg cgctctaaag cgggtcgcga
      121 tctttcagat tcgctcctcg cgctttcagt ctttgttttg gcgcatgtcg ttatcgcaaa
      181 accgctgcac acttttgcgc gacatgctct gatccccctc atctgggggg gcctatctga
      241 gggaatttcc gatccggctc gcctgaacca ttctgctttc cacgaacttg aaaacgctag
      301 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa
      361 taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa gggcgaggag
      421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      541 atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac
      601 ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc
      661 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      841 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      901 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      961 ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta ccagtccgcc
     1021 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1081 gccgggatca ctctcggcat ggacgagctg tacaagtaac tcggtaccaa attccagaaa
     1141 agaggcctcc cgaaaggggg gccttttttc gttttggtcc aatggcggcg cgccatcgaa
     1201 tgaggtgctt cctcgctcac tgactcgctg cacgaggcag acctcagcgc tagcggagtg
     1261 tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg
     1321 agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc
     1381 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa
     1441 cggggcggag atttcctgga agatgccagg aagatactta acagggaagt gagagggccg
     1501 cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct
     1561 caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg
     1621 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     1681 ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     1741 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
     1801 cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat
     1861 tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag
     1921 ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag
     1981 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg
     2041 cagaccaaaa cgatctcaag aagatcatct tattaagggg tctgacgctc agtggaacga
     2101 aaaatcaatc taaagtatat atgagtaaac ttggtctgac agttacctta gaaaaactca
     2161 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     2221 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     2281 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2341 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2401 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2461 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     2521 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2581 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2641 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2701 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     2761 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     2821 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     2881 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac
     2941 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     3001 tttattgttc atgatgatat atttttatct tgtgcaatgt acatcagaga ttttgagaca
     3061 caa
//
