LOCUS       V009541                 3902 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009541
VERSION     V009541
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3902)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3902)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..146
                     /label="PAcu"
                     /note="PAcu"
                     /regulatory_class="promoter"
     regulatory      108..113
                     /regulatory_class="minus_35_signal"
     misc_feature    114..135
                     /label="AcuO operator"
                     /note="AcuO operator"
     regulatory      131..136
                     /regulatory_class="minus_10_signal"
     misc_RNA        148..198
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      230..241
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             248..964
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      968..1028
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1033..1086
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1051..1056
                     /regulatory_class="minus_35_signal"
     regulatory      1074..1079
                     /regulatory_class="minus_10_signal"
     regulatory      1087..1114
                     /label="acu2"
                     /note="acu2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1115..1771
                     /gene="acuR"
                     /label="Transcriptional regulator AcuR"
                     /note="Transcriptional regulator AcuR from Cereibacter
                     sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
                     31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.).
                     Accession#: Q3J6K8"
     regulatory      1787..2041
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(2353..2898)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2990..3802)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgcgctagca agtaaggccg acgcttcaca accgcacttg atttaataga
      121 ccataccgtc tattatttct ggccatagct gtcaccggat gtgctttccg gtctgatgag
      181 tccgtgagga cgaaacagcc tctacaaata attttgttta atactagaga aagaggggaa
      241 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      361 cacctacggc aagctgaccc tgaagttcat ctgcaccaca ggcaagctgc ccgtgccctg
      421 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca
      481 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      721 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      781 gctcgccgac cactaccagc agaacacccc aatcggcgac ggccccgtgc tgctgcccga
      841 caaccactac cttagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      961 caagtaactc ggtaccaaat tccagaaaag aggcctcccg aaaggggggc cttttttcgt
     1021 tttggtccaa tggcggcgcg ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata
     1081 gcgcccggaa gagagtcaat tcagggaggt gaatatgccg ctgaccgaca ccccgccgtc
     1141 tgttccgcag aaaccgcgtc gtggtcgtcc gcgtggtgct ccggacgctt ctcttgctca
     1201 ccagtctctg atccgtgctg gtctggaaca cctgaccgaa aaaggttact cttcggttgg
     1261 tgttgacgaa atcctgaaag ctgctcgtgt tccgaaaggt tctttctacc actacttccg
     1321 taacaaagct gacttcggtc tggctctgat caaagcttac gacacctact tcgctcgtct
     1381 cctcgaccag gcgttcctgg acggttcgct ggctccgctg gctcgtctgc gtctgttcac
     1441 ccgtatggct gaagaaggta tggctcgtca cggtttccgt cgtggttgcc tggttggtaa
     1501 cctgggtcag gaaatgggcg ctctgccgga cgacttccgt gctgctctga tcggtgttct
     1561 ggaaacctgg caacatcgta ccgctcagct gttccgtgaa gctcaggctt gcggtgaact
     1621 gtctgctgac catgacccgg acgctctggc tgaagctttc tggatcggat gggaaggtgc
     1681 tatcctgcgt gctaaactgg aactgcgtcc ggacccgatg cactctttca cccgtacctt
     1741 cggtcgtcac ttcgttaccc gtacccagga ataatgataa ggatcctaat tggtaacgaa
     1801 tcagacaatt gacggctcga gggagtagca tagggtttgc agaatccctg cttcgtccat
     1861 ttgacaggca cattatgcat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg
     1921 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     1981 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcaaa tattttatct
     2041 gaggtgcttc ctcgctcact gactcgctgc acgaggcaga cctcagcgct agcggagtgt
     2101 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     2161 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     2221 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     2281 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     2341 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     2401 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     2461 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     2521 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     2581 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     2641 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     2701 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     2761 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     2821 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     2881 agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca gtggaacgaa
     2941 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccttag aaaaactcat
     3001 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     3061 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     3121 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     3181 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     3241 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     3301 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     3361 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     3421 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     3481 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     3541 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     3601 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     3661 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     3721 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg
     3781 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     3841 ttattgttca tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac
     3901 aa
//
