LOCUS       V009544                 4184 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009544
VERSION     V009544
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..188
                     /label="PSalTTC"
                     /note="PSalTTC"
                     /regulatory_class="promoter"
     misc_feature    110..140
                     /label="NahO operator"
                     /note="NahO operator"
     regulatory      154..159
                     /regulatory_class="minus_35_signal"
     regulatory      176..181
                     /regulatory_class="minus_10_signal"
     misc_RNA        190..240
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      272..283
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             290..1006
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      1010..1069
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1074..1127
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1092..1097
                     /regulatory_class="minus_35_signal"
     regulatory      1115..1120
                     /regulatory_class="minus_10_signal"
     regulatory      1128..1156
                     /label="nah2"
                     /note="nah2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1157..2056
                     /gene="nahR"
                     /label="HTH-type transcriptional activator NahR"
                     /note="HTH-type transcriptional activator NahR from
                     Pseudomonas putida. Accession#: P10183"
     regulatory      2069..2323
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(2635..3180)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3272..4084)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaattattg aaggcctccc taacgggggg cctttttttg tttctggtct cccgcttaac
       61 gatcgttggc tgggggcctc gcttgggtta ttgctggtgc ccggccgggc gcaatattca
      121 tgttgatgat ttattatata tcgagtggtg tatttattta tattgtttgc tccgttaccg
      181 ttattaacag ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag
      241 cctctacaaa taattttgtt taatactaga gaaagagggg aaatactaga tggtgagcaa
      301 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      361 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      421 cctgaagttc atctgcacca caggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      481 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt
      541 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      601 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      661 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      721 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      781 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      841 gcagaacacc ccaatcggcg acggccccgt gctgctgccc gacaaccact accttagcta
      901 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      961 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaac tcggtaccaa
     1021 attccagaaa agaggcctcc cgaaaggggg gcctttttcg ttttggtcca atggcggcgc
     1081 gccatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa
     1141 ttcatggggg ttgaatatgg aactgcgtga cctggattta aacctgctgg tggtgttcaa
     1201 ccagttgctg gtcgacagac gcgtctctgt cactgcggag aacctgggcc tgacccagcc
     1261 tgccgtgagc aatgcgctga aacgcctgcg cacctcgcta caggacccac tcttcgtgcg
     1321 cacacatcag ggaatggaac ccacacccta tgccgcgcat ctggccgagc acgtcacttc
     1381 ggccatgcac gcactgcgca acgccctaca gcaccatgaa agcttcgatc cgctgaccag
     1441 cgagcgtacc ttcaccctgg ccatgaccga cattggcgag atctacttca tgccgcggct
     1501 gatggatgcg ctggctcacc aggcccccaa ttgcgtgatc agtacggtgc gcgacagttc
     1561 gatgagcctg atgcaggcct tgcagaacgg aaccgtggac ttggccgtgg gcctgcttcc
     1621 caatctgcaa actggcttct ttcagcgccg gctgctccag aatcactacg tgtgcctatg
     1681 tcgcaaggac catccagtca cccgcgaacc cctgactctg gagcgcttct gttcctacgg
     1741 ccacgtgcgt gtcatcgccg ctggcaccgg ccacggcgag gtggacacgt acatgacacg
     1801 ggtcggcatc cggcgcgaca tccgtctgga agtgccgcac ttcgccgccg ttggccacat
     1861 cctccagcgc accgatctgc tcgccactgt gccgatatgt ttagccgact gctgcgtaga
     1921 gcccttcggc ctaagcgcct tgccgcaccc agtcgtcttg cctgaaatag ccatcaacat
     1981 gttctggcat gcgaagtacc acaaggacct agccaatatt tggttgcggc aactgatgtt
     2041 tgacctgttt acggattgat aaggatccta attggtaacg aatcagacaa ttgacggctc
     2101 gagggagtag catagggttt gcagaatccc tgcttcgtcc atttgacagg cacattatgc
     2161 atcgatgata agctgtcaaa catgagcaga tcctctacgc cggacgcatc gtggccggca
     2221 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     2281 atcgggctcg ccacttcggg ctcatgagca aatattttat ctgaggtgct tcctcgctca
     2341 ctgactcgct gcacgaggca gacctcagcg ctagcggagt gtatactggc ttactatgtt
     2401 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     2461 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     2521 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     2581 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     2641 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     2701 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     2761 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     2821 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     2881 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2941 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     3001 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     3061 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     3121 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     3181 gaagatcatc ttattaaggg gtctgacgct cagtggaacg aaaaatcaat ctaaagtata
     3241 tatgagtaaa cttggtctga cagttacctt agaaaaactc atcgagcatc aaatgaaact
     3301 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     3361 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     3421 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     3481 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     3541 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     3601 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     3661 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     3721 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     3781 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     3841 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     3901 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     3961 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     4021 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     4081 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     4141 tatttttatc ttgtgcaatg tacatcagag attttgagac acaa
//
